Culture and separation device and culture and separation method for daphnia magna
A separation device and a technology for large fleas, which are applied in animal husbandry and other directions, can solve the problems of inability to cultivate a large number of fleas, the quality of experiments cannot be guaranteed, aquatic organisms are susceptible to diseases, etc., and achieve convenient subculture, growth and reproduction. The effect of avoiding pollution
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Embodiment 1
[0045] Such as Figure 5Shown, the cultivating and separating device of Daphnia magna of the present invention, it comprises algae growth promoting device 1, places the Daphnia cultivating container 2 in the algae growing promoting device 1, places the circulating water device 4 in the Daphnia cultivating container 2, And flea separation device 3.
[0046] Among them, such as figure 1 As shown, the algae growth promoting device 1 includes a board layer 6 supported by the upper end of a bracket 7 , and an LED plant growth lamp 5 fixed on the lower surface of the board layer 6 . The material of the ply 6 is preferably wood. The material of the bracket 7 is preferably stainless steel. For better implementation of the present invention, the LED plant growth lamp 5 is facing the open top of the Daphnia cultivating container 2, and the area basically covers the opening at the top of the Daphnia cultivating container 2. The LED plant growth lamp 5 is controlled by a timing device...
Embodiment 2
[0051] A method for cultivating and isolating Daphnia magna using the device of Embodiment 1, the method may further comprise the steps:
[0052] A. Under the indoor condition of 20±2°C, inject 15L of water into the Daphnia magna culture container 2, the water surface is higher than the water inlet of the circulating water device 4, and lower than the water outlet of the circulating water device 4, and the circulating water device is operated for 41 days. The first process is equivalent to the storm process, but it will not change the pH value of the water body. Continue to add the algae food of the large flea to the large flea culture container 2, such as Chlorella or Scenedesmus etc., the food concentration reaches 10 5 / mL, run the circulating water device for 410 minutes.
[0053] B continues to add magna species into the magna culture container 2, turn on the LED plant growth lamp 5 on the algae growth promotion device 1, and run the circulating water device regularly fo...
Embodiment 3
[0058] Example 3: Flea reference substance test.
[0059] The Daphnia daphnia acute toxicity test is used to evaluate the possible impact of the test substance on aquatic organisms. Under the specified conditions, make Daphnia magna contact with aqueous solutions of different concentrations of the test substance, and determine the concentration of the test substance when 50% of the Daphnia magna dies. This test measures the acute toxicity of the reference substance potassium dichromate to Daphnia magna to determine whether the test organisms and test conditions meet the requirements.
[0060] 1 Materials and methods
[0061] 1.1 Daphnia magna species tested
[0062] Daphnia magna Straus, flea species domesticated by our laboratory. Experimental fleas were obtained according to the method in Example 2.
[0063] 1.2 Test conditions
[0064] Instruments and equipment: balance, dissolved oxygen meter, thermometer, pH meter, pipette, glassware, etc.
[0065] Test water parame...
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