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Culture and separation device and culture and separation method for daphnia magna

A separation device and a technology for large fleas, which are applied in animal husbandry and other directions, can solve the problems of inability to cultivate a large number of fleas, the quality of experiments cannot be guaranteed, aquatic organisms are susceptible to diseases, etc., and achieve convenient subculture, growth and reproduction. The effect of avoiding pollution

Inactive Publication Date: 2012-09-26
NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are no specific instructions on how to keep the fleas in the parthenogenetic state, how to ensure that the age of the fleas used in the test is between 6 and 24 hours, and how to ensure that the fleas do not leave the water during the test.
There are no specific reference methods for domestic flea testing units to ensure the quality of testing
Especially when the amount of experiments is large and the demand for fleas is large, there is no simple and easy-to-operate method
Scholar B. Rotter has done research on the cultivation method of Daphnia magna, which cannot satisfy the problem of cultivating a large number of isolated fleas.
The patent No. 201110047304 published by Huang Jianrong and others is "A Method for Cultivating and Preserving Daphnia Daphnia". The patent mainly establishes a simple flea-algae-snail-fish aquatic ecosystem to reserve and preserve species. During the actual operation, aquatic organisms are prone to diseases. The system must be rebuilt after being infected, and the quality of the experiment cannot be guaranteed

Method used

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  • Culture and separation device and culture and separation method for daphnia magna
  • Culture and separation device and culture and separation method for daphnia magna
  • Culture and separation device and culture and separation method for daphnia magna

Examples

Experimental program
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Effect test

Embodiment 1

[0045] Such as Figure 5Shown, the cultivating and separating device of Daphnia magna of the present invention, it comprises algae growth promoting device 1, places the Daphnia cultivating container 2 in the algae growing promoting device 1, places the circulating water device 4 in the Daphnia cultivating container 2, And flea separation device 3.

[0046] Among them, such as figure 1 As shown, the algae growth promoting device 1 includes a board layer 6 supported by the upper end of a bracket 7 , and an LED plant growth lamp 5 fixed on the lower surface of the board layer 6 . The material of the ply 6 is preferably wood. The material of the bracket 7 is preferably stainless steel. For better implementation of the present invention, the LED plant growth lamp 5 is facing the open top of the Daphnia cultivating container 2, and the area basically covers the opening at the top of the Daphnia cultivating container 2. The LED plant growth lamp 5 is controlled by a timing device...

Embodiment 2

[0051] A method for cultivating and isolating Daphnia magna using the device of Embodiment 1, the method may further comprise the steps:

[0052] A. Under the indoor condition of 20±2°C, inject 15L of water into the Daphnia magna culture container 2, the water surface is higher than the water inlet of the circulating water device 4, and lower than the water outlet of the circulating water device 4, and the circulating water device is operated for 41 days. The first process is equivalent to the storm process, but it will not change the pH value of the water body. Continue to add the algae food of the large flea to the large flea culture container 2, such as Chlorella or Scenedesmus etc., the food concentration reaches 10 5 / mL, run the circulating water device for 410 minutes.

[0053] B continues to add magna species into the magna culture container 2, turn on the LED plant growth lamp 5 on the algae growth promotion device 1, and run the circulating water device regularly fo...

Embodiment 3

[0058] Example 3: Flea reference substance test.

[0059] The Daphnia daphnia acute toxicity test is used to evaluate the possible impact of the test substance on aquatic organisms. Under the specified conditions, make Daphnia magna contact with aqueous solutions of different concentrations of the test substance, and determine the concentration of the test substance when 50% of the Daphnia magna dies. This test measures the acute toxicity of the reference substance potassium dichromate to Daphnia magna to determine whether the test organisms and test conditions meet the requirements.

[0060] 1 Materials and methods

[0061] 1.1 Daphnia magna species tested

[0062] Daphnia magna Straus, flea species domesticated by our laboratory. Experimental fleas were obtained according to the method in Example 2.

[0063] 1.2 Test conditions

[0064] Instruments and equipment: balance, dissolved oxygen meter, thermometer, pH meter, pipette, glassware, etc.

[0065] Test water parame...

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Abstract

The invention discloses a culture and separation device for daphnia magna, comprising an algae growth promoting device, a daphnia magna culture container arranged in the algae growth promoting device, a circulating water device arranged in the daphnia magna culture container, and a daphnia magna separation device. The invention also discloses a culture and separation method for daphnia magna by utilizing the device. The culture and separation device provided by the invention is simple and convenient for operation, and high in operability; the materials of the device are easy to be obtained; and the device is simple in structure, and convenient for manufacturing and maintenance. The culture container employed by the culture and separation device for daphnia magna is formed through one step and small in volume, thereby avoiding pollution of other substances and being easy for replacement, and also facilitating subculture; the flea food growth promoting device is advantageous for the growth and reproduction of flea food algae, thereby omitting the work of feeding each day; the circulating water device reduces the step of aeration needed in the flea culture process; and the separation device prevents fleas from leaving the water surface in the separation process to guarantee large-quantity quick separation of fleas. The application of the culture and separation device for daphnia magna can ensure the quality of flea toxicity tests.

Description

technical field [0001] The invention relates to a method for cultivating and separating zooplankton, in particular to a device for cultivating and separating magna magna and a method for cultivating and separating the same. Background technique [0002] Fleas belong to the invertebrate phylum Arthropoda, Crustacea, and Cladocera. They are important groups of freshwater zooplankton and are the bait for many important economic fishes. At the same time, they play a pivotal role in the self-purification of waters. Daphnia magna has the advantages of short life cycle, fast reproduction, economy, convenience, sensitivity to poisons, and easy cultivation in the laboratory. In addition to their importance in aquatic ecosystems, they have been applied in many countries and have become a A standard test indicator organism, widely used in aquatic toxicity tests. [0003] At present, the United States, the European Union, Japan and other countries and international organizations have e...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 续卫利周军英单正军韩志华焦少俊王蕾
Owner NANJING INST OF ENVIRONMENTAL SCI MINIST OF ECOLOGY & ENVIRONMENT OF THE PEOPLES REPUBLIC OF CHINA
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