Wall-attachment cell culture method

Abstract

The invention provides a wall-attachment cell culture method. The method comprises the following steps of: a, inoculation, wherein cell inoculum in a cell inoculation bottle is fed into a wall-attachment cell culture device by circulation of a peristaltic pump, and vertically passes through a cell growing layer and a cell trapping layer, so that the cell is closely contacted with the cell growing layer, and the cell is adhered to the cell growing layer after standing for a period in a constant temperature environment; b, culture, wherein the flowing direction of culture solution is parallel to the cell growing layer through the peristaltic pump in a stable culture condition, while nutrition is supplied to the cell, cell metabolized products are carried away; and c, digestion. The method has simple requirements on equipment and convenience in operation, and is easily implemented as long as a plurality of incubators are connected in series or parallel when the culture scale is required to be enlarged. The wall-attachment cell culture method can be widely applied in multiple fields, such as cell seed screening, culture solution development, gene medicinal expression and production and vaccine research and production, and has great marketing value.

Description

technical field [0001] The invention specifically relates to an adherent cell culture method for large-scale in vitro culture of mammalian animal cells, insect cells, plant cells and human cells. Background technique [0002] The cultivation of cells by large-scale in vitro culture technology is an effective method for the production of biological products. In the 1960s and 1970s, the microcarrier culture system and hollow fiber cell culture technology for large-scale culture of animal cells were established. In recent decades, due to the sharp increase in human demand for biological products such as growth hormone, interferon, monoclonal antibodies, vaccines, and interleukins, the amount of biological products obtained from animal tissues using traditional biochemical techniques has been far from being sufficient. Meet the needs. As the principles and methods of cell culture are becoming more and more perfect, the large-scale cell culture technology is gradually becoming ...

AI Technical Summary

Problems solved by technology

At present, domestic enterprises cannot fully grasp the technology of microcarrier technology. Only a few enterprises can achieve the cultivation scale of 300L, and only a few enterprises in the world have mastered the cultivation technology of more than 1000L, and cannot exceed the cultivation scale of 5000L, which seriously restricts vaccines. and bioengineering drug research and production

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