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Technique for heart disease external differentiation therapy by utilizing stem cells of masticatory muscles and orbicularis oculi muscles

An orbicularis oculi muscle and masticatory muscle technology, applied in the field of adult stem cells and regenerative medicine, can solve the problems of non-differentiation, clinical application limitation, cardiomyocyte differential electrical coupling, etc., and achieve the effect of improving the conversion rate

Inactive Publication Date: 2012-10-03
珠海霍普金斯医药研究院股份有限公司
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  • Summary
  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Although skeletal muscle stem cells have the advantages of being convenient to obtain materials and a large amount of in vitro expansion, they do not have the ability to differentiate cardiomyocytes in vivo and poor electrical coupling, which greatly limits their clinical application.

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] specific implementation plan

[0021] Materials and methods

[0022] Masticatory muscle sample processing and masticatory muscle stem cell growth and culture

[0023] Firstly, the masticatory muscle tissue was obtained by subcutaneous biopsy under sterile conditions. Place the tissue in PBS solution on ice (under this condition it can be stored for up to 12 hours). Use sterile forceps and scissors to cut the masticatory muscle tissue into 0~5 mm 3 Fragments were digested with type II collagenase (1mg / ml) at 30-40°C for 25-40 minutes. After digestion, centrifuge masticatory muscle tissue, use a pipette to gently disperse tissue cell aggregates, and put them into culture medium (containing 15% heat-inactivated fetal calf serum, 100 Units / ml penicillin G, 100 μg / ml streptomycin, 2 mmol / l L-glutamine, and 0.1 mmol / l 2-mercaptoethanol in DMEM). At 30~40℃ 5% CO 2 Suspension culture in the incubator.

[0024] Masticatory muscle stem cell expansion and liquid nitro...

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PUM

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Abstract

The invention belongs to the field of adult stem cells and regenerative medicine, and relates to the techniques of adult stem cell extraction, external expansion and differentiation induction as well as stem cell transplantation. The techniques are based on the theory that head and facial masticatory muscles have the same origin with cardiac progenitor cells. As shown by the analysis on the genetic expression, cardiac muscle genes Nk*2.5 and Isl1 in the masticatory muscle stem cells have expressions of different degrees. The techniques utilize that the masticatory muscle stem cells have the characteristics of the cardiac muscle stem cells, collect a masticatory muscle sample by the non-invasive biopsy method, and externally expands the masticatory muscle stem cells by the suspension culture method; and non-coding micro RNA (ribonucleic acid) is adopted to change the masticatory muscle stem cells and facilitate differentiation and purification of the masticatory muscle stem cells into the heart muscle cells. The transplantation of the purified cardiac muscle cells to a part with myocardial infarction is performed by means of the coronary artery duct injection or the cell membrane technique (the cell membrane is placed on the surface of myocardial infarction organization), so that the myocardial infarction or the ischemic heart disease can be cured.

Description

technical field [0001] The invention belongs to the field of adult stem cells and regenerative medicine. It involves adult stem cell extraction, in vitro expansion, induced differentiation and stem cell transplantation technology. Background technique [0002] Cardiovascular disease has become the leading cause of death in most countries, including developed countries and China. The application of stem cells in the treatment of myocardial infarction and ischemic heart disease is a new field of cardiovascular disease treatment and will eventually become a mainstream technology. [0003] The types of stem cells currently used in clinical trials include bone marrow stem cells, skeletal muscle stem cells, peripheral and umbilical cord blood stem cells, and umbilical cord mesenchymal stem cells. So far, bone marrow stem cells and skeletal muscle stem cells have completed clinical phase III trials. Although skeletal muscle stem cells have the advantages of being convenient to o...

Claims

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Application Information

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IPC IPC(8): C12N5/074C12N5/071A61K35/34A61P9/10A61D7/00
Inventor 滕国奇王义刚赵柏松
Owner 珠海霍普金斯医药研究院股份有限公司
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