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Synsepalum dulcificum Denill tree branch and leaf extract and extraction method and application of extract

An extraction method and technology of miracle fruit, which are used in the preparation of antioxidants and anti-gout drugs, the branch and leaf extract of miracle fruit and the field of extraction thereof, can solve problems such as unhealthy health, and achieve abundant plant resources and significant xanthine oxidation. Enzyme inhibitory activity and high extraction yield

Inactive Publication Date: 2012-10-24
万福群
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Other drugs have similar adverse health side effects

Method used

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  • Synsepalum dulcificum Denill tree branch and leaf extract and extraction method and application of extract
  • Synsepalum dulcificum Denill tree branch and leaf extract and extraction method and application of extract
  • Synsepalum dulcificum Denill tree branch and leaf extract and extraction method and application of extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step (1) Pulverize the branches and leaves of the miracle fruit respectively to obtain the extracted raw materials, which are divided into 4 equal parts;

[0029] Step (2) Add 50%, 70%, 0%, and 90% ethanol to the raw materials for extracting the branches and leaves of the miracle fruit, respectively, reflux for 5 hours, distill under reduced pressure, and freeze-dry to obtain eight kinds of powders respectively.

[0030] Determination of the above-mentioned powder components and their extraction rate and content by conventional methods, the determination method is as follows:

[0031] Drawing of the standard curve: Weigh 9.4mg of gallic acid dried at 120°C with constant weight, and distilled water to 100ml. Accurately measure 0.1ml, 0.2ml, 0.3ml, 0.4ml, 0.5ml, 0.6ml, 0.7ml, 0.8ml of gallic acid solution respectively, put them in a 10ml volumetric flask, add water to about 6ml, shake well, and add Folin-Ciocalteu reagent 0.5ml, shake well, add 1.5ml of 20% sodium carbon...

Embodiment 2

[0043] Step (3) get the 70% ethanol extract powder that embodiment 1 step (2) gained is dissolved in water, process through HP-20 macroporous adsorption resin, then adopt concentration respectively to be 0%, 10%, 30%, 50% , 70%, 90% volume ethanol aqueous solution, the amount of the eluent is respectively 4, 3, 4, 5, 4, 6 times of the volume of the column bed to obtain the collected solution;

[0044] Step (4) Vacuum-concentrate the collected solution to 1 / 7, 1 / 6, 1 / 5, 1 / 4, 1 / 3, 1 / 3 of the original volume respectively to obtain a concentrated solution, freeze-dry for 20 hours to obtain refined branches and leaves Extract dry powder.

[0045] Determination of branch and leaf extract dry powder recovery rate, total phenol recovery rate, the method is the same as in Example 1, and the results are shown in Table 3.

[0046] table 3

[0047]

[0048]Table 3 shows that after separation by macroporous resin, the recovery rate of dry powder and total phenols of miracle fruit bran...

Embodiment 3

[0056] The 30% ethanol aqueous solution obtained by embodiment 2 is used for measuring the xanthine oxidase inhibitory activity under its different aqueous solution concentration, the result is as follows: image 3 As shown, at a lower concentration (0.5 mg / mL aqueous solution), the activity of the extract dry powder solution was lower (not more than 40%). With the increase of concentration, from 0.5 to 5 mg / mL, the inhibitory activity of dry powder solution on xanthine oxidase increased continuously. At a higher concentration (5 mg / mL), the xanthine oxidase inhibitory activity of the extract dry powder solution can exceed 80%.

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Abstract

The invention relates to an extraction method for a Synsepalum dulcificum Denill tree branch and leaf extract. The extraction method comprises the following steps of: crushing Synsepalum dulcificum Denill tree branches and leaves to obtain an extracted raw material; adding ethanol water; performing reflux extraction for 2-5 hours; filtering; performing reduced pressure distillation on supernate; performing freeze drying to obtain branch and leaf crude extract dry powder; dissolving the branch and leaf crude extract dry powder in water; performing macroporous resin treatment; then, eluting by using the ethanol water to obtain drips; and respectively performing vacuum concentration and the freeze drying to obtain a refined branch and leaf extract. The total phenol content of the obtained extract is 66.5 percent, and the total phenol content of a branch extract is 84.1 percent. The diphenylpicrylphenylhydrazine (DPPH) scavenging activity of the extract is 7.9 and 7.8 times of that of glutathione, and the oxygen radical absorbing capacity of the extract is 7.0 and 6.2 times of that of the glutathione. The extract is good in inhibitory activity on xanthine oxidase and can be used for preparing antioxidants, gout suppressants and health-care products.

Description

technical field [0001] The invention relates to extraction technology, in particular to the branch and leaf extract of the miracle fruit tree and its extraction method; the invention also relates to the application of the extract in the preparation of antioxidants and anti-gout drugs. Background technique [0002] Miracle fruit (Synsepalum dulcificum Denill) is native to Ghana and Congo in West Africa. It is a tropical evergreen shrub native to tropical regions of West Africa. In the 1960s, it was introduced into tropical and subtropical regions such as Hainan, Yunnan, and Guangxi for planting. The English name of the mysterious fruit is Mysterious Fruit (mysterious fruit) or Miracle Fruit (unbelievable fruit). The magic of the mysterious fruit is to transform the extremely sour fruit into a sweet and sweet fruit. It can be used as a food aid for acidic foods, or as a taste-changing agent that can meet the sweetness needs of diabetics. In addition, phenolic acid compounds ...

Claims

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Application Information

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IPC IPC(8): A61K36/185A61P19/06A61P39/06A23L1/29A61K127/00A23L33/00
Inventor 赵谋明万福群
Owner 万福群
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