Reagent kit for fast detection of drainage oil and method for fast detection of drainage oil

A technology of waste oil and kit, which is applied in the directions of biochemical equipment and methods, microbial determination/inspection, etc., can solve the problems of lack of waste oil detection methods, and achieve good economic and social benefits, protection of interests, and significant promotion and application value. Effect

Inactive Publication Date: 2012-10-31
GUANGZHOU QUALITY SUPERVISION & TESTING INST
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, there is still a lack of detection methods that can effectively identify waste oil in China.

Method used

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  • Reagent kit for fast detection of drainage oil and method for fast detection of drainage oil
  • Reagent kit for fast detection of drainage oil and method for fast detection of drainage oil
  • Reagent kit for fast detection of drainage oil and method for fast detection of drainage oil

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 The kit for rapid detection of waste oil

[0042] Contains the following components (for 50 PCR reactions):

[0043] (1) DNA extraction buffer (100mL)

[0044] Weigh 2.00mg CTAB (cetyltrimethylammonium bromide), 1.21mg Tris.HCl (trishydroxymethylaminomethane hydrochloride), 0.58g EDTA2Na (disodium ethylenediaminetetraacetic acid) and 8.18 g NaCl in a volumetric flask, dilute to 100mL with water, and mix well. After sterilizing at 120° C. for 30 minutes at high temperature, 10.00 mg of protease K (purchased from Dalian Bao Biology Co., Ltd.) was added;

[0045] (2) Taq DNA polymerase (25 μl);

[0046] Purchase 25 μl of Taq DNA polymerase, a total of 125 IU (purchased from Dalian Bao Biological Company);

[0047] (3) PCR reaction solution (1000μl)

[0048] Firstly, primers were prepared, and the prepared primer concentration was 100 nmol / L.

[0049] Primers were prepared according to conventional methods in the art. Synthesis was carried out on an automat...

Embodiment 2

[0077] Embodiment 2 Utilizes the kit of embodiment 1 to detect the gene component in waste oil

[0078] The detection method comprises the following steps:

[0079] (1) Preparation of sample DNA

[0080] a Suspected gutter oil and edible vegetable oil samples: take 300-500mL samples;

[0081] Take a centrifuge tube, add 50mL oil sample and 50mL double-distilled water to each tube, shake on the oscillator for 60min, centrifuge at 12000r / min for 10min, discard the upper layer of oil, and keep the lower layer of water. Then add 50 mL of oil sample to the centrifuge tube, continue to shake, and centrifuge. The above process is repeated 8 times, and the volume of the extracted oil sample is about 400 mL. After extraction until the lower aqueous phase is turbid, centrifuge at 12000r / min for 10min, discard the upper layer of grease, transfer the aqueous phase to a flat dish, concentrate and dry, and the drying and concentration process takes about 3 hours; after fully drying, add 2...

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Abstract

The invention discloses a reagent kit for fast detection of drainage oil and a method for fast detection of the drainage oil. The reagent kit comprises a deoxyribonucleic acid (DNA) extraction buffer, thermus aquaticus DNA (Taq DNA) polymerase, a polymerase chain reaction (PCR) solution and a sample loading buffer. According to the reagent kit, on the basis of existing research, key dominant factors such as extraction reagents and reaction conditions in the whole process from extraction of drainage oil genes to PCR detection are improved and optimized, and the whole set of drainage oil detection reagent kit which is high in stability, fast and simple is produced. According to the reagent kit and the detection method, constituents of the drainage oil are identified from the point of genes, the detection of the drainage oil has the advantages of being high in specificity, high in sensitivity, good in method reproducibility, fast and simple to detect and the like, and the gap of existing detection methods is filled. The reagent kit and the detection method have important popularization and application values and are important to effective supervision of the drainage oil and protection of benefit of consumers.

Description

technical field [0001] The invention relates to a kit for rapidly detecting waste oil and a detection method thereof. Background technique [0002] At present, the quality of cooking oil in the market is uneven. In order to save costs and make huge profits, a small number of oil processing manufacturers illegally process and refine waste oil, or mix waste oil in ordinary cooking oil to deceive consumers. Food safety has brought hidden dangers and seriously endangered the health and interests of consumers. [0003] Waste oil is a non-edible oil with extremely poor quality and hygiene, and its peroxide value, acid value, moisture, carbonyl value, malondialdehyde and other indicators seriously exceed the standard. It is generally divided into 3 categories: [0004] The first is gutter oil in a narrow sense, which refers to oil that is simply processed and extracted from greasy floating matter in sewers or leftovers and leftovers (commonly known as swill) in hotels and restaura...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 郭新东吴玉銮柯振华陈立伟罗海英侯向昶冼燕萍陈意光罗东辉吴文海王斌
Owner GUANGZHOU QUALITY SUPERVISION & TESTING INST
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