Microfluidic protein chip for detecting genetically modified crops and kit of microfluidic protein chip

A protein chip and detection kit technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of unknown transgenic products damaging trade reputation, health and safety threats, etc., to avoid the decline of probe activity, convenient operation, The effect of high sensitivity
CN102759628AInactive Publication Date: 2012-10-31ZHEJIANG UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
ZHEJIANG UNIV
Publication Date
2012-10-31
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a microfluidic protein chip for high-through detecting genetically modified crops. The microfluidic protein chip comprises a nucleic acid chip which is synthesized on the basis of mu ParafloTM microfluidic protein chip and contains six nucleotide sequences and 6 types of antibody-oligonucleotide composite probes, thereby simultaneously detecting 6 transgenetic proteins of trans-bacillus thuringiensis Bt insecticidal crystal protein (Cry1Ac), trans-bacillus thuringiensis Bt insecticidal crystal protein (Cry1c), trans-bacillus thuringiensis Bt insecticidal crystal protein (Cry1F), trans-bacillus thuringiensis Bt insecticidal crystal protein (Cry1Ab), soybean trypsin (SBTI) and neomycin phosphate transferase II gene (NPTII). The invention further provides a transgenic assay kit which comprises a mu ParafloTM microfluidic deoxyribose nucleic acid (DNA) chip which contains six types of probes, six types of antibody-oligonucleotide composite probes, six types of detection antibodies and the like.
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Description

technical field

[0001] The invention relates to the technical field of transgenic component detection and microfluidic protein chips, in particular to a high-throughput microfluidic protein chip and related kits for detecting multiple transgenic components. Background technique

[0002] Over the past decade, transgenic technology has been extensively developed. Researchers have used transgenic technology to breed herbicide-resistant, drought-resistant, salt-alkali-resistant, heavy metal-resistant and pest-resistant varieties with high nutritional value, which play an important role in increasing food production, reducing post-harvest losses and increasing the nutritional value of agricultural products. With the continuous research, absorption and popularization of transgenic crops, the diversity of genetically modified crops is becoming more and more abundant. Many countries and regions have successively approved the commercial production of genetically modified crops, and ...

Claims

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