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Method for separating and detecting six cortical hormones in skin-care cosmetic

A technology for cosmetics and hormones, applied in the field of analysis, can solve the problems of high price of liquid chromatograph and liquid chromatographic column, complex sample pretreatment process, and high operating cost of the instrument

Inactive Publication Date: 2012-11-14
JIANGNAN UNIV
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  • Application Information

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Problems solved by technology

[0003] The determination methods of glucocorticoids mainly include high performance liquid chromatography, LC-MS, GC-MS and capillary electrokinetic chromatography, among which high performance liquid chromatography is the most commonly used method, but this method requires relatively complicated sample pretreatment process, and the liquid chromatograph and liquid chromatographic column are expensive, and the operating cost of the instrument is relatively high

Method used

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  • Method for separating and detecting six cortical hormones in skin-care cosmetic
  • Method for separating and detecting six cortical hormones in skin-care cosmetic
  • Method for separating and detecting six cortical hormones in skin-care cosmetic

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Experimental program
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Embodiment

[0028] 1. Materials and methods

[0029] (1) Test material:

[0030] Lotion in a certain brand of cosmetics.

[0031] (2) Instrument and chromatographic conditions:

[0032] TH-3100 high-efficiency capillary electrophoresis instrument.

[0033] Using uncoated quartz capillary (total length 65cm, effective length 50cm, column inner diameter 50μm) as the separation channel, the ionic liquid BMIM-BF 4 The microemulsion with β-cyclodextrin as the additive is the running buffer solution, and the cathodic injection is carried out under the pressure of -20kV for 3s, the ultraviolet detection wavelength is 245nm, the running voltage is -20kV, and the temperature is 20°C.

[0034] (3) Preparation of hormone standard solution:

[0035] Accurately weigh 0.0800 g each of prednisone, hydrocortisone, cortisone acetate, fluticasone propionate, triamcinolone acetonide, and dexamethasone, add 10.0 mL methanol to dissolve, mix well, and prepare a mass concentration of 8 mg / The standard st...

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Abstract

The invention provides a method for separating and detecting six cortical hormones in a skin-care cosmetic. The six cortical hormones, i.e. fluticasone propionate, triamcinolone acetonide, cortisone acetate, dexamethasone, hydrocortisone and prednisone, in the cosmetic are separated and detected by a reverse micro emulsion electrokinetic chromatography (MEEKC) with ionic liquid and beta-cyclodextrin as additives. Compared with a conventional MEEKC separation and detection method, the method has higher separation degree. An experiment result shows that the method is simple in sample treatment, low in using amount, economic, high in speed, and high in separation degree and sensitivity. Capillary electrophoretic detection on the cortical hormones in the cosmetic is realized by the method.

Description

technical field [0001] The invention belongs to the technical field of analysis, and in particular relates to a method for separating and measuring corticosteroids in skin care cosmetics. technical background [0002] Glucocorticoids (Corticosteroids) are a class of steroid hormones secreted by the adrenal cortex, which have important physiological activities, such as anti-inflammatory and anti-allergic, reducing the permeability of capillary walls and cell membranes, and reducing inflammatory exudation. Short-term use of skin care cosmetics containing glucocorticoids can make the skin smooth and delicate, ruddy, white and tender, moisturizing and elastic, reduce wrinkles or treat acne, and has a good cosmetic effect. However, long-term use of glucocorticoids can cause facial skin damage, skin thinning, telangiectasia, and hair follicle atrophy. my country's "Hygienic Standards for Cosmetics" ] Such substances are stipulated as prohibited substances in cosmetics. Therefor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
Inventor 李娟陈新商少明李萌刘瑛
Owner JIANGNAN UNIV
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