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Method for detecting autoantibodies of systemic lupus erythematosus patient

A lupus erythematosus and autoantibody technology, applied in the medical field, can solve the problems of complex diagnostic methods for systemic lupus erythematosus, and achieve the effect of improving detection efficiency and diagnosis rate

Inactive Publication Date: 2012-12-12
SUZHOU HEALTH COLLEGE
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AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for detecting autoantibodies in patients with systemic lupus erythematosus, which solves the problem that the diagnostic methods for systemic lupus erythematosus in the prior art are relatively complicated. The problem

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  • Method for detecting autoantibodies of systemic lupus erythematosus patient

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Embodiment 1

[0015] From 2008 to 2010, 109 patients with systemic lupus erythematosus who were outpatients or inpatients in Suzhou Municipal Hospital were used as the test objects, including 95 females and 14 males, aged 14-82 years, with an average age of 43 years. All patients with systemic lupus erythematosus met the diagnostic criteria of the American College of Rheumatology.

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Abstract

The invention discloses a method for detecting autoantibodies of a systemic lupus erythematosus patient. The method includes steps of: detecting antinuclear antibodies and double-strand deoxyribonucleic acid (DNA) of the systemic lupus erythematosus patient respectively in an indirect immunofluorescence method, measuring antinuclear antibodies spectrums in a euroimmun western blotting method, and finally detecting single-strand DNA of the systemic lupus erythematosus patient through an enzyme-linked immunosorbent assay. The method for detecting autoantibodies of the systemic lupus erythematosus patient simplifies the existing detection method, improves detection efficiency and accurate diagnosis rate, and is beneficial to clinical diagnosis and treatment of systemic lupus erythematosus.

Description

technical field [0001] The invention relates to the medical field, in particular to a method for detecting autoantibodies of patients with systemic lupus erythematosus. [0002] Background technique [0003] Systemic lupus erythematosus is an autoimmune disease with sudden or slow onset and invasion of multiple systems and organs. Its main immunological feature is the production of various autoantibodies and the formation of immune complexes to cause disease. The detection of autoantibodies is an important indicator for the diagnosis of systemic lupus erythematosus. At present, many hospitals in China have carried out the detection of autoantibodies. It is of high clinical value for the diagnosis and treatment of systemic lupus erythematosus to clarify the titer and fluorescence pattern of antinuclear antibody in patients with systemic lupus erythematosus and the relationship between them and various autoantibodies. [0004] Based on the above problems, inventing a metho...

Claims

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Application Information

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IPC IPC(8): G01N33/564
Inventor 黄静芳徐红星
Owner SUZHOU HEALTH COLLEGE
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