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Targeted ultrasonic phase-change bimodal imaging nano contrast agent and preparation method and application thereof

A nano-contrast agent, dual-modality technology, applied in the field of ultrasound imaging and nuclear magnetic imaging, can solve the problems of complex operation, low imaging clarity and resolution, and long imaging process.

Active Publication Date: 2019-11-05
SHANGHAI INST OF ONCOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Ultrasound imaging has the advantages of continuous and dynamic observation of tissues, not limited by imaging layers, no radiation or trauma to the human body, imaging equipment can be portable, easy to operate, and low in price in practical applications; it also has the advantages of imaging clarity and Disadvantages of lower resolution
Therefore, ultrasound contrast agents, which can improve the imaging effect to a certain extent, have emerged as the times require, but their imaging duration is short, and targeted imaging cannot be achieved, so the imaging clarity and resolution are still limited
MRI has good clarity and resolution, no radiation, no trauma, but the imaging process is long and the operation is complicated

Method used

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  • Targeted ultrasonic phase-change bimodal imaging nano contrast agent and preparation method and application thereof
  • Targeted ultrasonic phase-change bimodal imaging nano contrast agent and preparation method and application thereof
  • Targeted ultrasonic phase-change bimodal imaging nano contrast agent and preparation method and application thereof

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preparation example Construction

[0105] The preparation method of the present invention is an emulsified solvent volatilization method, and there is no specific requirement on the molecular weight of PLGA-mPEG or PLGA-PEG-Mal. The current commercial materials of this type only need to be dissolved in the selected organic solvent. The ratio of the raw material lactic acid and glycolic acid of the PLGA has no special requirements, as long as it meets the condition of being soluble in an organic solvent. As the molecular weight of the polymer increases, the particle size of the prepared contrast agent may also increase, and when it reaches a certain level, it may break through the nanometer level and is not suitable for in vivo imaging. Those skilled in the art can According to such hints, the upper limit of the molecular weight is selected according to the specific situation.

[0106] application

[0107] The liquid perfluorocarbon wrapped in the ultrasonic phase-change dual-mode imaging nano-contrast agent pr...

Embodiment 1

[0119] Preparation of targeted ultrasonic phase-change dual-modality imaging nanocontrast agent (PFH / siRNA / Fe 3 o 4 @Pt(IV)NPs-cRGD)

[0120] 1) Dissolution: Weigh 10.2mg PLGA-mPEG (molecular weight: 12500, Jinan Shandong Daigang Biotechnology Co., Ltd.), 6.0mg PLGA-PEG-Mal (molecular weight: 12000, Jinan Shandong Daigang Biotechnology Co., Ltd.) with an electronic balance, 1.8mgDOTAP, 6.0mg DSPE-PEG-Pt(Ⅳ) (DSPE-PEG-NH2, molecular weight: 1000, Shanghai Pengshuo Biotechnology Co., Ltd., connected with Pt(Ⅳ) laboratory) were completely dissolved in 0.6mL chloroform at room temperature, dissolved The process is kept in a sealed state, and PFH is fully dissolved in the organic phase;

[0121] 2) Ultrasound: Add 80 μL of 1.5 nmol gene (siRNA sequence: CTGGGAGCCAGATGACGAC, Guangzhou Ruibo Biotechnology Co., Ltd.) and 0.92 mg of iron to the organic phase after being modified by DEAPA and positively charged on the surface. 3 o 4 The DEPC aqueous phase of nanoparticles, ultrasonic...

Embodiment 2

[0127] Characterization and performance determination of targeted ultrasonic phase-change dual-modality imaging nanocontrast agents

[0128] 1. Characterization of the targeted ultrasonic phase-change dual-modality imaging nano-contrast agent prepared in Example 1

[0129] (1) The appearance of the contrast agent is uniform and transparent brown, and there is no obvious layering after standing.

[0130](2) The particle size of nanoparticles detected by the Malvern laser particle size analyzer is about 307.9±19.80nm, which makes it possible for them to pass through the tumor capillary endothelial gap (100-780nm), so as to achieve passive targeting and satisfy tumor targeting. Like the particle size requirements of the contrast agent; its particle size polydispersity index PDI is 0.273±0.02, indicating that its particle size is relatively uniform; the nanoparticle Zeta potential is 10.9±0.31mV, which is beneficial to the cells after targeted positioning. Uptake, increase the co...

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Abstract

The invention discloses a targeted ultrasonic phase-change bimodal imaging nano contrast agent and a preparation method and an application thereof. The ultrasonic phase-change bimodal imaging nano contrast agent comprises a shell membrane and a core coated in the shell membrane, the core comprises Fe3O4 nanoparticles and liquid PFH (perfluorohexane), and the shell membrane comprises PLGA and phospholipid.

Description

technical field [0001] The invention relates to the fields of ultrasonic imaging and nuclear magnetic imaging, in particular to a targeted ultrasonic phase-change dual-mode imaging nano-contrast agent and its preparation method and application. Background technique [0002] Ultrasound imaging has the advantages of continuous and dynamic observation of tissues, not limited by imaging layers, no radiation or trauma to the human body, imaging equipment can be portable, easy to operate, and low in price in practical applications; at the same time, it also has the advantages of imaging clarity and The disadvantage of lower resolution. Therefore, ultrasound contrast agents, which can improve the imaging effect to a certain extent, have emerged as the times require, but their imaging duration is short, and targeted imaging cannot be achieved, so the imaging clarity and resolution are still limited. MRI has good clarity and resolution, no radiation, no trauma, but the imaging proce...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K49/12A61K49/10A61K49/14A61K49/18A61K49/22B82Y5/00B82Y15/00A61K31/7105A61K47/52A61K47/69A61K47/54A61K47/62A61K9/51A61K47/02A61K47/34A61K47/24A61K47/18
CPCA61K49/0002A61K49/128A61K49/10A61K49/14A61K49/1857A61K49/1839A61K49/1866A61K49/221A61K49/225B82Y5/00B82Y15/00A61K31/7105A61K47/52A61K47/6937A61K47/544A61K47/62A61K9/5115A61K9/5153A61K9/5123A61K9/5169
Inventor 段友容孙颖张燕华赵梦
Owner SHANGHAI INST OF ONCOLOGY
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