Beta-glucosidase and application thereof

A glucosidase and nucleotide technology, applied in beta-glucosidase and its application fields, can solve the problems of complex components, unsuitable extraction, low enzyme activity, etc., and achieve the effect of huge commercial value

Active Publication Date: 2012-12-19
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, the enzyme activity of β-glucosidase in liquid fermentation is generally low; and the productio

Method used

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  • Beta-glucosidase and application thereof
  • Beta-glucosidase and application thereof
  • Beta-glucosidase and application thereof

Examples

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Embodiment Construction

[0021] The present invention will be described in further detail below in conjunction with the accompanying drawings and embodiments. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0022] 1. Screening and detection of β-glucosidase

[0023] 1. Extraction of the total DNA of Grape ear mold paper (purchased from China Microbiology Culture Collection Committee, CGMCC 3.5365) total DNA

[0024] (1) Use CMC liquid medium to culture grape ear mold papery at 30°C and 200rpm for 48 hours on a shaking table;

[0025] (2) Filter the mycelium with sterile gauze, and grind the mycelium into powder in liquid nitrogen.

[0026] (3) Refer to the kit (Fungal DNA kit) for the extraction method.

[0027] 2. Amplification of β-glucosidase gene

[0028] A pair of oligonucleotide primers P1 and P2 were designed according to the β-glucosidase gene sequence obtained by analyzing the whole genome of Staphylo...

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Abstract

The invention relates to a beta-glucosidase and an application thereof, wherein the beta-glucosidase comprises: a, enzyme with the sequence of SEQ ID NO:1; and b, enzyme which is derived from the enzyme in step a through substituting, deleting or adding one or a plurality of amino acids and has the activity of the enzyme in the step a. The selected beta-glucosidase provided by the invention has the deoxyglucose and hygromycin resistance and transglycosylation, under the transglycosylation, cellobiose and other oligosaccharides can generate sophorose and other inductors, and enter cells through a constitution permease system on a cell membrane, and the synthesis of cellulose in the cells is initiated. Consequently, the beta-glucosidase can be used for the production of catalytically synthesizing the sophorose to induce the cellulose, and thereby having a greater commercial value.

Description

technical field [0001] The invention belongs to the technical field of enzyme gene and enzyme engineering, and specifically relates to a beta-glucosidase and its application. Background technique [0002] The English name of β-glucosidase (EC 3.2.1.21) is β-glucosidase, also known as β-D-glucoside hydrolase, also known as gentiobiase, cellobiase and amygdalinase. Belonging to the exohydrolase class, it is a class of enzymes that catalyze the transfer of glycosides between nucleophilic molecules, and can be used in short-chain oligosaccharides or cellobiose, between residues of carbohydrates with aromatic groups or alkyl groups Interruption of β-1,4-glycosidic bonds. β-glucosidase is a kind of cellulase system, and it is the rate-limiting enzyme of cellulose degradation. In the process of cellulase hydrolysis, it degrades various oligosaccharides and cellobiose into glucose, and is the last key enzyme. It plays a key role in saccharification and hydrolysis of cellulose. β-...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12N15/56C12N15/63C12N15/70C12N15/74C12N1/15C12N1/19C12N1/21
Inventor 王华明于海玲
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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