Water disinfectant for aquiculture and use method thereof

A technology of aquaculture and disinfectant, which is applied in the direction of disinfectant, botany equipment and method, sterilization/microdynamic water/sewage treatment, etc. It can solve the problems of single preparation, limited disinfectant, corrosion of utensils, etc., and achieve aquaculture Animal safety, various modes of action, and simple preparation

Inactive Publication Date: 2012-12-26
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problem of aquatic diseases has always been a serious problem hindering the healthy development of the aquaculture industry
From the current point of view, there are still many shortcomings in existing disinfectants, such as chlorine-containing disinfectants are easily affected by changes in water organic matter and pH, and have a corrosive effect on instruments and are also highly toxic to fish; quaternary ammonium salts The bactericidal effect of iodine preparations is easily affected by water hardness; the effect of iodine preparations is easily affected by conditions such as light and temperature, and is prone to volatilization and failure (Huang Xiaoli and Deng Yongqiang, 2004)
[0003] At present, most of the disinfectants used in production are of single type and single preparation, and most of them are derived from human medicine, veterinary medicine and water treatment agents, and the disinfectants specially developed for aquaculture ponds and aquaculture animals are still very limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Prepare a kind of water body disinfectant for aquaculture ponds according to the weight percentages of the following components:

[0014] Potassium monopersulfate, 75%; Sulfamic acid, 15%; Malic acid, 3%; Sodium chloride, 2%; Sodium polymetaphosphate, 1%; Coptis chinensis extract, 4%.

[0015] The above composition can be uniformly mixed according to weight percentage.

[0016] Using the water body disinfectant described in Example 1 above, an experiment on the killing effect of Aeromonas was carried out. The disinfectant was formulated into a disinfectant solution with a concentration of 0.5ppm; the medium was beef extract liquid and solid medium, pH 7.0±0.2; all reagents used were of analytical grade. The test strain is the preparation of the suspension of Aeromonas standard strain (Aeromonas hydrophila) ATCC7966 (purchased from China Agricultural Microorganism Collection Center). Each strain of purely cultured Aeromonas was cultured in single-fold broth, cultured o...

Embodiment 2

[0018] Prepare a kind of water body disinfectant for aquaculture ponds according to the weight percentages of the following components:

[0019] Potassium monopersulfate, 50%; Sulfamic acid, 15%; Malic acid, 15%; Sodium chloride, 10%; Sodium polymetaphosphate, 3%; Coptis chinensis extract, 7%.

[0020] The above composition can be uniformly mixed according to weight percentage.

[0021] Use the water body disinfectant described in embodiment 2, carry out the experiment of prawn cultivation in May, 2010 in a prawn pond in Xiaoshan, Hangzhou. 15 days before the prawn seedlings are stocked, a disinfectant solution is prepared according to a concentration of 1% to fully disinfect the pond. Then put in the prawn fry and breed according to the normal steps. Every 15 days after the seedlings were put out, a disinfectant was prepared at a concentration of 0.5 ppm for routine disinfection of the shrimp ponds. The experiment was carried out for a total of 123 days. During the experim...

Embodiment 3

[0024] Prepare a kind of water body disinfectant for aquaculture ponds according to the weight percentages of the following components:

[0025] Potassium monopersulfate, 45%; Sulfamic Acid, 25%; Malic Acid, 15%; Sodium Chloride, 2%; Sodium Polymetaphosphate, 3%; Coptis Rhizoma Extract, 10%.

[0026] The above composition can be uniformly mixed according to weight percentage.

[0027] Using the water disinfectant described in Example 3, an experiment on the killing effect on grass carp reovirus (GCRV) was carried out. Grass carp reovirus was cultured with grass carp kidney (CIK) cells, and the maintenance medium was serum-free DMEM medium (containing TPCK-treated trypsin 2g / ml, 2% fetal bovine serum). The median infectious dose (TCID50) of virus to cells was calculated according to Reed-Meuench. Use maintenance solution to serially dilute disinfectant to 12 dilutions, add to CIK cell 96-well culture plate, cultivate in 26° incubator, and observe cell changes every day. Dete...

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PUM

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Abstract

The invention relates to a disinfectant for an aquiculture pond. The water disinfectant for the aquiculture pond comprises the following components in percentage by weight: 45-75 percent of potassium monopersulfate, 15-25 percent of sulfamic acid, 3-15 percent of malic acid, 2-10 percent of sodium chloride, 1-3 percent of sodium polymetaphosphate and 4-10 percent of coptis extract. The water disinfectant for the aquiculture pond provided by the invention has the characteristics of high efficiency, low toxicity, no residue and no pollution.

Description

technical field [0001] The invention relates to a disinfectant, in particular to a disinfectant for aquaculture ponds. Background technique [0002] China is a big country in aquaculture. According to statistics, my country's aquaculture production accounts for more than two-thirds of the world's total aquaculture production. However, the problem of aquatic diseases has always been a serious problem hindering the healthy development of the aquaculture industry. According to incomplete statistics, the annual incidence of aquaculture diseases in my country is more than 50%, and the loss rate is as high as 20%, causing direct economic losses of more than 10 billion yuan. In recent years, with the development of high-density intensive farming, aquatic diseases have become more and more serious, which has caused huge economic losses to the aquaculture industry, and the losses caused by viral diseases have also increased day by day. In the process of aquatic disease prevention ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N65/32A01N59/02A01N59/00A01N25/30A01P1/00C02F1/50
Inventor 许丹吕利群沈晓宝
Owner SHANGHAI OCEAN UNIV
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