Diluted rabbit semen preserving fluid
A technology for preserving liquid and semen, which is applied to the preservation, application, and animal husbandry of human or animal bodies, and can solve the problems of restricting the development of the rabbit industry, failing to fully reflect the benefits of large-scale breeding, and low production efficiency of rabbits.
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[0009] The present invention will be described in detail below in conjunction with specific embodiments.
[0010] 1. Test materials and methods
[0011] 1.1 Test animals
[0012] Choose 30 adult Zika large New Zealand male rabbits aged 1-2 years old with no disease and normal reproductive ability.
[0013] 1.2 Test equipment and reagents
[0014] Test equipment: microscope, electronic balance, PH meter, constant temperature water bath, high pressure sterilizer, refrigerator, aseptic operating table, micro pipette gun, sperm collection device, blood cell counting board and other glass instruments.
[0015] Test reagent: Coomassie brilliant blue staining solution, Tris, citric acid, anhydrous glucose (analytically pure), egg yolk, penicillin, streptomycin, distilled water, 5% (weight percent) glucose solution, normal saline, 3% (weight percent) Percentage) Nacl solution, etc.
[0016] 1.3 Determination of semen quality
[0017] 1.3.1 Semen Collection
[0018] Artificial se...
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