Diluted rabbit semen preserving fluid

A technology for preserving liquid and semen, which is applied to the preservation, application, and animal husbandry of human or animal bodies, and can solve the problems of restricting the development of the rabbit industry, failing to fully reflect the benefits of large-scale breeding, and low production efficiency of rabbits.

Inactive Publication Date: 2013-01-02
SICHUAN ANIMAL SCI ACAD
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Problems solved by technology

In the past 30 years, my country's animal husbandry has achieved considerable development, but the technical level of the rabbit industry is still relatively lagging behind compared with other livestock and poultry industries, especially in terms of reproductive technology, pigs, cattle, chickens, etc. have already achieved artificial insemination for breeding, but At present

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  • Diluted rabbit semen preserving fluid
  • Diluted rabbit semen preserving fluid
  • Diluted rabbit semen preserving fluid

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Embodiment Construction

[0009] The present invention will be described in detail below in conjunction with specific embodiments.

[0010] 1. Test materials and methods

[0011] 1.1 Test animals

[0012] Choose 30 adult Zika large New Zealand male rabbits aged 1-2 years old with no disease and normal reproductive ability.

[0013] 1.2 Test equipment and reagents

[0014] Test equipment: microscope, electronic balance, PH meter, constant temperature water bath, high pressure sterilizer, refrigerator, aseptic operating table, micro pipette gun, sperm collection device, blood cell counting board and other glass instruments.

[0015] Test reagent: Coomassie brilliant blue staining solution, Tris, citric acid, anhydrous glucose (analytically pure), egg yolk, penicillin, streptomycin, distilled water, 5% (weight percent) glucose solution, normal saline, 3% (weight percent) Percentage) Nacl solution, etc.

[0016] 1.3 Determination of semen quality

[0017] 1.3.1 Semen Collection

[0018] Artificial se...

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Abstract

The invention discloses a diluted rabbit semen preserving fluid. The diluted rabbit semen preserving fluid comprises the components including 2.13g of citric acid, 3.17g of Tris and 0.60g of anhydrous glucose, and is prepared by dissolving the components into 100ml of distilled water, and then autoclaving, placing the solution into a sterile operation desk after the solution is autoclaved and cooled, and adding 2ml of yolk, 200 thousands IU of penicillin and 200 thousands IU of streptomycin. The test results show that the self-mixed diluted rabbit semen preserving fluid is capable of storing the male rabbit semen for above 60 hours at 4 DEG C in vitro, and the activity is more than 0.5 (the activity of 0.5 is an index which can guarantee normal artificial insemination). The in vitro storage capability of the diluted rabbit semen preserving fluid is far higher than 5% of glucose solution and normal saline. Simultaneously, the pregnancy rate of the artificial insemination test is about 75% when the activity is 0.5, and the test results also show that the artificial insemination can be carried out when the rabbit semen is stored in vitro within 60 hours in the formula.

Description

technical field [0001] The invention relates to a rabbit semen dilution preservation solution. Background technique [0002] Rabbit is a herbivorous animal, and it is currently an agricultural project supported by the state to promote the restructuring of the animal husbandry industry, and it is also a characteristic industry for increasing income and wealth in rural areas of our country. In the past 30 years, my country's animal husbandry has achieved considerable development, but the technical level of the rabbit industry is still relatively lagging behind compared with other livestock and poultry industries, especially in terms of reproductive technology, pigs, cattle, chickens, etc. have already achieved artificial insemination for breeding, but At present, the domestic rabbit industry basically adopts the natural mating method for reproduction, which seriously restricts the development of the domestic rabbit industry. [0003] For a long time, due to the lack of breakth...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 任永军郑洁杨超邝良德谢晓红雷岷李丛艳郭志强张翔宇邓小东张翠霞
Owner SICHUAN ANIMAL SCI ACAD
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