Kit for fast culture, identification and drug sensitivity test of helicobacter pylori (Hp) and examination method therefor

Abstract

The invention belongs to the technical field of helicobacter pylori (Hp) examination and discloses a kit for fast culture, identification and a drug sensitivity test of Hp and an examination method therefor. The kit comprises a first culture tube, an identification reagent device and a drug sensitivity test unit. Through the first culture tube of Hp, bedside inoculation of a specimen is realized and the traditional sample transport and preservation processes are avoided; ordinary thermostat culture is realized and dependence on a CO2 incubator is eliminated; an Hp growth result is represented by a color change so that a result can be determined accurately by laypeople; through the combination of a growth promoting agent and a growth indicator, culture time is shortened and fast culture of Hp is realized; an Hp identification reagent, fast combination identification of Hp is realized; and through the drug sensitivity test unit of Hp, fast and accurate examination of drug sensitivity of Hp is realized. The kit and the examination method therefor can realize fast and accurate examination of Hp.

Description

technical field [0001] The invention belongs to the technical field of gastric Helicobacter pylori inspection, and in particular relates to a kit for rapid culture, identification and drug sensitivity of gastric Helicobacter pylori and a method for using the kit to inspect gastric Helicobacter pylori. Background technique [0002] Helicobacter pylori (Hp for short) is a Gram-negative bacterium with unipolar, multi-flagellated, blunt-rounded, and spirally curved ends, and clinical infection is very common. Hp is the main pathogenic factor of chronic active gastritis, peptic ulcer, gastric mucosa-associated lymphoid tissue (MALT) lymphoma and gastric cancer. In 1994, the World Health Organization / International Agency for Research on Cancer classified Hp as a class I carcinogen. [0003] According to the detection purpose, clinical Hp detection can be divided into two categories: diagnostic detection and therapeutic detection. The purpose of "diagnostic testing" is to find out...

AI Technical Summary

Problems solved by technology

[0012] E-Test method: directly read the minimum inhibitory concentration (MIC) according to the size of the inhibition zone around the E-Test test strip on the agar plate.

[0013] Agar dilution method: determine the MIC of Hp according to the growth of the same concentration of bacterial liquid on the culture plate of different concentrations of drugs, which is the gold standard for evaluating other Hp drug susceptibility test methods, but this method is complicated to operate and the workload is huge, so it is not suitable clinical use;

[0014] Broth dilution method: determine the MIC of Hp according to the growth of the same concentration of bacterial liquid in the culture solution of different concentrations of drugs, which is the gold standard for evaluating other Hp drug susceptibility testing methods, but this method is complicated to operate and requires a large amount of work. suitable for clinical use