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Method for utilizing culture technology to produce Solomon turmeric and red tulip seedlings

A technology of Solomon turmeric and Hongyan, which is applied in the field of producing Solomon turmeric and Hongyan turmeric flower seedlings by thin slice culture technology, can solve the problems of low reproduction coefficient, difficulty in meeting large-scale production needs and circulation requirements, and achieve high output , less investment, to avoid the effect of weakened growth

Active Publication Date: 2013-02-06
GUANGDONG UNIV OF EDUCATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, cutting rhizomes are used for propagation, but the reproduction coefficient is very low in the natural state, and it is difficult to meet the needs of large-scale production and circulation.
So far, there is no research report on the rapid propagation technology of Hongyan turmeric

Method used

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  • Method for utilizing culture technology to produce Solomon turmeric and red tulip seedlings
  • Method for utilizing culture technology to produce Solomon turmeric and red tulip seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] (1) Disinfection of explants and induction of adventitious buds: take the new shoots germinated from the rhizomes of Solomon turmeric (Curcuma soloensis Voel, obtained from the Guangzhou Agricultural Technology Extension Center. The same below.), rinse with tap water and use Soak in detergent for 30 minutes, then rinse with running water for 30 minutes, then disinfect the surface with 70wt% alcohol for 60 seconds, and finally disinfect with 0.1wt% mercury chloride for 19 minutes, cut 0.5cm buds on the ultra-clean workbench as explants body, inoculated into shoot induction medium A for culture, the culture conditions were temperature 26°C, 30 μmol·m -2 the s -1 Adventitious buds were induced by culturing for 15 days under light intensity (white fluorescent light) and 16L / 8D light conditions; the described bud induction medium A was MS medium + 3mg·L -1 6-BA+0.2mg·L -1 NAA+30g·L -1 Sucrose+7g·L -1 Agar, pH 5.9, 121°C, 1.06kg / cm 2 Sterilize for 15 minutes;

[0051] (...

Embodiment 2

[0057] (1) Disinfection of explants and induction of adventitious buds: Take the new shoots germinated from the rhizomes of Solomon Turmeric, rinse them with tap water, soak them in detergent for 30 minutes, rinse them with tap water for 30 minutes, and wash them with 75wt% Disinfect the surface with alcohol for 75 seconds, and finally disinfect with 0.1wt% mercuric chloride for 17 minutes. Cut out 1 cm buds on the ultra-clean workbench as explants, inoculate them into bud induction medium A for cultivation, and the cultivation condition is 27°C , 30μmol·m -2 the s -1 Adventitious buds were induced by culturing for 20 days under light intensity (white fluorescent light) and 16L / 8D light conditions; the described bud induction medium A was MS medium + 3mg·L -1 6-BA+0.2mg·L -1 NAA+30g·L -1 Sucrose+7g·L -1 Agar, pH 5.8, 121°C, 1.06kg / cm 2 Sterilize for 15 minutes;

[0058] (2) Seedling formation from adventitious buds: transfer the adventitious buds induced in step (1) into...

Embodiment 3

[0063] (1) Disinfection of explants and induction of adventitious buds: Take the new shoots germinated from the rhizomes of Solomon Turmeric, rinse them with tap water, soak them in detergent for 30 minutes, rinse them with tap water for 30 minutes, and wash them with 72wt% Alcohol was used to disinfect the surface for 90 seconds, and finally sterilized with 0.1wt% mercuric chloride for 15 minutes. Cut out 1.5 cm buds on the ultra-clean workbench as explants, inoculate them into bud induction medium A for cultivation, and the cultivation conditions were temperature 28 ℃, 30μmol·m -2 the s -1 Adventitious buds were induced by culturing for 18 days under light intensity (white fluorescent light) and 16L / 8D light conditions; the described bud induction medium A was MS medium + 3mg·L -1 6-BA+0.2mg·L -1 NAA+30g·L -1 Sucrose+7g·L -1 Agar, pH 5.7, 121°C, 1.06kg / cm 2 Sterilize for 15 minutes;

[0064] (2) Seedling formation from adventitious buds: transfer the adventitious buds ...

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Abstract

The invention discloses a method for utilizing culture technology to produce Solomon turmeric and red tulip seedlings and an application thereof. The method comprises the steps as follows: the plant slide culture technology is utilized, explants are sampled and sterilized, adventitious buds are induced, slices are cut and seeding is performed, so as to obtain the Solomon turmeric and red tulip seedlings, the seedling propagating is quick and the propagating coefficient is high; the density of the used plant growth regulator 6-BA is rather low, the cost is lower, the yield is high, and the phenomena of weak growth, small and thin stems and leaves and vitrification of the tissue culture seedlings due to overhigh cytokinin content in the culture medium are avoided. By adopting the invention, through one and half year circulating expanding propagation, the effective rates of the Solomon turmeric and red tulip seedlings still amount to 95%, and the Solomon turmeric can bloom after being transplanted outdoor for 3.5 to 4.5 months. The technology utilizing culture technology to produce Solomon turmeric and red tulip seedlings provide an effective path for enabling the Solomon turmeric and red tulip seedlings to be large-scaled and marketized.

Description

technical field [0001] The invention belongs to the technical field of flower seedling production of Zingiberaceae (Curcuma), Solomon turmeric (Curcumasoloensis Valeton) and Hongyan turmeric (Curcuma rubescence Roxb.), and particularly relates to a method of producing Solomon turmeric and Hongyan turmeric (Curcuma rubescence Roxb.) by using thin slice culture technology. How to grow tulip flower seedlings. Through the method, a large amount of Solomon curcuma longa and Hongyan turmeric test-tube seedlings can be quickly obtained by using the sheet culture technology, and then the test-tube seedlings are transplanted into ziplock bags for bagging culture, thereby obtaining a large amount of Solomon turmeric and Hongyan turmeric flower seedlings. Background technique [0002] Solomon turmeric is a perennial rhizome-shaped herbaceous flower plant of the ginger family Curcuma longa. It is native to the Solomon Islands. It has been introduced and cultivated in Guangdong, my count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 肖望涂红艳黄斯娜管敏燕陈爱葵关见留
Owner GUANGDONG UNIV OF EDUCATION
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