Method for extracting gymnemic acid from gymnema sylvestre

A technology of gymnemic acid and gymnasia sylvestre, applied in the field of natural medicinal chemistry, can solve the problems of difficulty in recovering n-butanol, low product purity, etc., and achieve the effects of being beneficial to purification, high purity and less impurities

Inactive Publication Date: 2013-02-06
苏州宝泽堂医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, in the document "Technology Research on Macroporous Adsorption Resin Adsorption and Purification of Gymnemic Acid", 60-70% of Gymnemic Acid is purified by macroporous resin, and the product purity is low
The extraction method mostly uses n-butanol extraction, and it is difficult to recover n-butanol

Method used

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  • Method for extracting gymnemic acid from gymnema sylvestre

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Effect test

Embodiment 1

[0015] Crush the Gymnema raw material, take 5kg, put it into a small extraction tank, add 50kg of 30% ethanol solution to soak for 10 hours, filter out the liquid and soak in an equal volume of solution for 8 hours, combine the extracts and filter, add a hollow cellulose membrane with a molecular weight cut off of 2000 Ultrafiltration, filtration at room temperature, adding an appropriate amount of deionized water for dialysis, collecting the permeate to recover ethanol, adding the concentrated solution to a 3L D315 weakly basic anion exchange resin column, after the adsorption is completed, take deionized water to wash and colorless, and then take 18L 50% The active ingredient was eluted with ethanol solution, and the eluate was collected and added with 1 kg of activated carbon to reflux for decolorization for 1 hour, the activated carbon was filtered off, concentrated under reduced pressure to 500 ml, left to crystallize, filtered and dried to obtain 105 g, with a content of 9...

Embodiment 2

[0017] Gymnema raw material pulverizes, gets 5kg, puts into small extraction tank, adds 30kg90% ethanol solution and soaks for 6 hours, extracts 3 times, adds the hollow cellulose membrane ultrafiltration of molecular weight cut-off 6000 after merging extracting solution, filters under normal temperature, adds Appropriate amount of deionized water for dialysis, collect the permeate to recover ethanol, add the concentrated solution to a 3L D315 weakly basic anion exchange resin column, after the adsorption is completed, take deionized water to wash and colorless, then take 9L of 80% ethanol solution to elute the active ingredients, collect The eluent was added with 800 g of activated carbon and refluxed for decolorization for 1 hour, the activated carbon was filtered off, concentrated under reduced pressure to 300 ml, left to crystallize, filtered out and dried to obtain 113 g with a content of 85% (UV method).

Embodiment 3

[0019] Gymnema raw material is crushed, take 10kg, put into a small extraction tank, add 80kg of 50% ethanol solution to soak for 10 hours, filter out the liquid and then soak in an equal volume of solution for 8 hours, combine the extracts and filter, then add a hollow cellulose membrane with a molecular weight cut-off of 3000 Ultrafiltration, filtration at room temperature, add appropriate amount of deionized water for dialysis, collect the permeate to recover ethanol, add the concentrated solution to 6L D315 weakly basic anion exchange resin column, after the adsorption is completed, take deionized water to wash and colorless, and then take 18L of 70% The active ingredient was eluted with ethanol solution, and the eluate was collected and added with 1.5 kg of activated carbon to reflux for decolorization for 1 hour, the activated carbon was filtered off, concentrated under reduced pressure to 500 ml, left to crystallize, filtered and dried to obtain 222 g, with a content of 8...

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Abstract

The invention belongs to the field of natural medicinal chemistry, and discloses a method for extracting gymnemic acid from gymnema sylvestre. The method comprises the steps that: a gymnema sylvestre raw material is crushed; 6-10 times of 30-90% ethanol is added for soak extraction 2-3 times; an extraction liquid is filtered and is subjected to ultrafiltration in an ultrafiltration membrane system; ethanol in a permeate is recovered, and the filtrate is subjected to absorption in anion exchange resin; the material is washed by using deionized water, until the material is colorless; the material is eluted by using 3-6BV 50-80% ethanol solution; active carbon is added fro reflux decolorization; an elution liquid is subjected to reduced-pressure standing crystallization; the material is filtered and dried, such that gymnemic acid is obtained. With the gymnemic acid production method provided by the invention, the process operation is simple, energy consumption is low, product purity is high, and the method is suitable for industrialized productions.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, and in particular relates to a method for extracting gymnemic acid from Gymnema syringae. Background technique [0002] Gymnema is a plant of the genus Gymnema in the family Astragalus, also known as Gymnema vine, King Kong vine, snake vine, and rice dipper. The roots and whole plants are used as medicine to clear away heat, detoxify, dispel wind and relieve pain. It is often used for rheumatic joint pain, carbuncle furuncle, poisonous swelling, and poisonous snake bites. Gymnema has been used in the folk for a long time, and it is used in Indian folk for anti-tumor, antimalarial, diuretic and hypoglycemic. [0003] Gymnemic acid (Gymnemic Acid, GA) is a series of triterpene saponins. Its structural general formula is: [0004] [0005] Scholars at home and abroad have found that gymnemic acid has the effects of lowering blood sugar, anti-caries and inhibiting sweetness response. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J63/00
Inventor 李法庆刘东锋
Owner 苏州宝泽堂医药科技有限公司
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