Phosphorus-dissolving stain PAN 4 and application of phosphorus-dissolving stain PAN 4 in promoting growth of walnuts

A technology for bacterial strains and phosphorus removal, applied in the field of microorganisms, can solve the problem of large demand for phosphorus elements, and achieve the effects of efficient growth promotion, plant growth promotion, growth promotion and optimization of root micro-ecological environment.

Inactive Publication Date: 2015-02-18
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the growth of walnut plants requires a large amount of phosphorus, and phosphorus is an important factor restricting its growth. At present, there is no report on the application of Pseudomonas vancouverensis Mohn et al as a phosphorus-dissolving bacteria agent on walnuts.

Method used

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  • Phosphorus-dissolving stain PAN 4 and application of phosphorus-dissolving stain PAN 4 in promoting growth of walnuts
  • Phosphorus-dissolving stain PAN 4 and application of phosphorus-dissolving stain PAN 4 in promoting growth of walnuts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0007] Example 1 Fermentation of Pseudomonas vancouverensis Mohn et al PAN 4 (CGMCC NO.6546) liquid preparation:

[0008] ① First-class slant seeds: make beef extract peptone (peptone 10 g, beef extract 3 g, NaCl 5 g, agar 15 g, distilled water 1000 mL, pH 7.0) slant medium by conventional methods, and inoculate PAN4 at 26-28°C Cultivate for 24 hours to make first-class seeds.

[0009] ②Secondary liquid seeds: phosphate-solubilizing bacteria medium [glucose 10 g, (NH 4 ) 2 SO 4 0.5 g, NaCl 0.3 g, KCl 0.3 g, FeSO 4 ·7H 2 O 0.03 g, MnSO 4 ·H 2 O 0.03 g, MgSO 4 ·7H 2 O 0.3 g, KH 2 PO 4 2.0 g, distilled water 1000 mL]) after autoclaving, bottle according to the proportion of 75mL liquid in a 300mL triangular flask (oxygen control), inoculate PAN4 slant seeds in a sterile state, inoculate 1 slant seed per bottle, temperature 26 -28°C, initial pH value 7.5, 120r / min shaking culture for 36h, to make PAN4 liquid seeds.

[0010] ③ Liquid fermentation: glucose 20 g / L, (NH ...

Embodiment 2

[0015] The experiment was carried out in the forest germplasm nursery of Sichuan Agricultural University from March 2009. During the growth period of walnut seedlings, routine management was carried out, and the location of flower pots was regularly rotated. The test soil was collected from the forest tree breeding base of Daxing Town, Ya'an City, Sichuan Province, and the sampling depth was 0-20 cm, sandy loam. The physical and chemical properties of the soil were measured as follows: soil pH 7.1, organic matter content 10.43 g / kg, total nitrogen content 0.80 g / kg, total phosphorus content 0.34 g / kg, available phosphorus content 4.50 mg / kg. The sample soil was air-dried and passed through a 1 mm sieve, and mixed with river sand passed through a 1 mm sieve at a ratio of 3:1 (V:V) for pot experiments. After the walnut seedlings were transplanted and survived, inject 50 mL of the liquid bacterial agent in the above-mentioned embodiment 1 with a sterile syringe around the walnut ...

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Abstract

The invention discloses a phosphorus-dissolving stain PAN 4, which is preserved in China General Microbiological Culture Collection Center of Institute of Microbiology of Chinese Academy of Sciences (CGMCC) in 3#, 1# Institute, West Beichen Road, Chaoyang District, Beijing City on 11, September, 2012, wherein the preservation number is CGMCC NO.6546; the classification name is Pseudomonas vancouverensis. The Pseudomonas vancouverensis Mohn et al PAN 4 stain in the invention has strong phosphorus-dissolving ability, effectively promotes growth of walnuts, improves rhizospheric microorganism environment, promotes growth of plants, improves photosynthetic physiological characteristic and phosphorus nutrient of the walnut seedlings, and has double functions of promoting and optimizing rhizospheric microorganism environment.

Description

technical field [0001] The present invention relates to the field of microbes, in particular to Pseudomonas vancouverensis Mohn et al phosphate solubilizing strain PAN 4 and its application in walnut growth promotion. Background technique [0002] Phosphorus is an essential nutrient element for plant growth and development. Phosphorus deficiency can lead to a significant decrease in crop yields. Although the total phosphorus content in the soil is high, it mostly exists in the soil in an insoluble state, which is difficult to be absorbed and utilized by plants. Applying phosphate fertilizer to solve the problem of soil phosphorus deficiency in production will not only deplete the limited phosphate rock resources, but also may cause environmental pollution and destroy the ecological balance. Phosphate solubilizing bacteria are beneficial microorganisms that can convert phosphorus that is difficult for plants to absorb in the soil into available phosphorus. The absorption of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01G7/06C12R1/38
Inventor 朱天辉李姝江余旋张丽娜谯天敏朴春根崔晓亮
Owner SICHUAN AGRI UNIV
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