Amplification composite for detecting microdeletion of Y-chromosome and detection kit
A chromosomal microdeletion and detection kit technology, applied in the field of biotechnology detection, can solve the problems of inconvenient sample detection, low sensitivity, inability to detect deletions and duplications, etc.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment Construction
[0095] The invention will be further described below in conjunction with specific embodiments and accompanying drawings. The present invention can be understood more clearly through the examples and drawings, but it is worth emphasizing that they do not limit the protection scope of the present invention.
[0096] Example 1 (8-position)
[0097] 1. Human Genomic DNA Extraction:
[0098] The DNA in 100 μl of anticoagulated whole blood was extracted using a DNA extraction kit, and the operation steps were in accordance with the instructions. After the DNA was extracted, it was quantified with an ultraviolet spectrophotometer and diluted to 2 ng / μl.
[0099] 2. Multiplex PCR amplification:
[0100] Dissolve a total of 8 pairs of primers for 8 sites and make a working solution with a concentration of 10 μM, and then prepare a primer mix (Primer mix) according to the volume ratio in Table 2: one amplification reaction completes all 8 sites point expansion. See the table below f...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com