Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for producing recombinant human interferon-alpha-2b-BCG (bacillus calmette guerin)

A technology of recombinant human interferon and recombinant interferon, which is applied in the field of genetic engineering to achieve the effects of easy detection, controllable parameters of culture conditions, and avoiding pollution

Active Publication Date: 2014-01-29
韩瑞发 +2
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention is to solve the problem of industrialized mass production of recombinant human interferon-α-2b-BCG, and provides a production method of recombinant human interferon-α-2b-BCG

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for producing recombinant human interferon-alpha-2b-BCG (bacillus calmette guerin)
  • Method for producing recombinant human interferon-alpha-2b-BCG (bacillus calmette guerin)
  • Method for producing recombinant human interferon-alpha-2b-BCG (bacillus calmette guerin)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0026] one. production steps, see figure 1 .

[0027] 1. seed activation

[0028] Freeze or cryopreserve the liquid culture recovery of recombinant interferon-α-2b-BCG seed batches, and inoculate 50 mL of freshly prepared improved Sutong potato liquid medium containing 30 μl / ml kanamycin medium, constant temperature 35-38°C, rotation speed 100-200rpm, and saturated humidity for 7-14 days to obtain primary activated seed liquid.

[0029] 2. Secondary Seed Preparation

[0030] Inoculate the fresh activated seed solution into 50mL of the improved Sutong potato liquid medium at a ratio of 1-4%, containing 30 microliters / ml of kanamycin, shake it in a 2000mL flask, and store it at 35~39℃ and saturated humidity , 100~200rpm shaking culture for 7~9 days to obtain secondary seeds (concentration up to standard OD 600 A value = 2).

[0031] 3. Ferment...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for producing recombinant human interferon-alpha-2b-BCG (bacillus calmette guerin). The method is that a recombinant interferon-alpha-2b-BCG strain is inoculated into an improved sauton potato fluid medium which contains kanamycin of 30 microliters / milliliter, so that a first-level activated seed fluid is obtained, and the first-level activated seed fluid is then inoculated into the improved sauton potato fluid medium according to the ratio of 1-4%, so that a second-level seed fluid with the OD (optical density) 600 A value of 2 is obtained; and the improved sauton potato fluid medium in a fermentation tank is autoclaved, is added with the second-level seed fluid according to the ratio of 2-6% and is fermented for 7-10 days, fermentation liquor is collected and centrifuged, precipitate is washed by phosphate buffer solution (PBS), and the strain is obtained after secondary centrifugation. The single production quantity of the produced recombinant human interferon-alpha-2b-BCG is large, the recombinant human interferon-alpha-2b-BCG is large is easy to detect during the growing process, the parameters of culture conditions can be controlled, and the pH, oxygen and nutrient substance requirements during the culture process can be regulated according to the growing process, so that the enrichment interspersed strains are directly obtained, and the operations of grinding and the like which can easily cause pollution are avoided.

Description

technical field [0001] The invention relates to genetic engineering technology, in particular to a production method suitable for industrialized production of recombinant human interferon-α-2b-BCG. Background technique [0002] Bacillus Calmette-Guerin (BCG) is a biological immunomodulator formed by the highly virulent Mycobacterium bovis. It is widely used to prevent tuberculosis all over the world. It has been proved to have a high degree of safety and few serious complications. Intravesical infusion of BCG has achieved satisfactory clinical efficacy in the prevention of tumor recurrence, treatment of residual tumors and carcinoma in situ, but 30%-45% of patients still do not respond to intravesical BCG infusion. Long-term follow-up Even up to 50% of patients. In addition, intracavitary BCG perfusion can also cause local and systemic reactions of the bladder. Serious complications may occur in 5% of patients, and 0.5% may even be life-threatening. This also limits the us...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/21C12R1/32
Inventor 孙二琳韩瑞发
Owner 韩瑞发
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com