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Preparation method of microsphere reagent

A technology of microspheres and reagents, applied in the field of medical testing, can solve the problems of cumbersome operation, high production cost, waste of manpower and material resources, etc., and achieve the effect of simple operation, large single-time production, and improved production efficiency

Pending Publication Date: 2020-03-27
AILEX TECH GRP CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this preparation method must undergo repeated centrifugation operations, which is not only cumbersome to operate, but also greatly wastes manpower and material resources, and the production cost is high.

Method used

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  • Preparation method of microsphere reagent
  • Preparation method of microsphere reagent

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Experimental program
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Effect test

Embodiment 1

[0092] This embodiment provides a microsphere reagent and its preparation method.

[0093] Preparation:

[0094] 1) Dissolving styrene microspheres in the first auxiliary agent to obtain a microsphere solution, wherein, in the microsphere solution, the mass concentration of the microspheres is 0.3%;

[0095] 2) Dissolving the serum amyloid A antibody in the first auxiliary agent to obtain a serum amyloid A antibody solution, in which the serum amyloid A antibody has a mass concentration of 0.3%;

[0096] 3) dissolving EDC and NHS in the second auxiliary agent to obtain a coupling agent solution, wherein, in the coupling agent solution, the total mass concentration of EDC and NHS is 5 mg / L;

[0097] 4) Mix the microsphere solution, the serum amyloid A antibody solution and the coupling agent solution for 12 hours, the rotation speed is 35rpm, the ultrasonic dispersion is performed, the ultrasonic power is 35%, the ultrasonic on time is 2s, the ultrasonic off time is 5s, and th...

Embodiment 2

[0101] This embodiment provides a microsphere reagent and its preparation method.

[0102] Preparation:

[0103] 1) Dissolving styrene microspheres in the first auxiliary agent to obtain a microsphere solution, wherein, in the microsphere solution, the mass concentration of the microspheres is 0.5%;

[0104] 2) dissolving the serum amyloid A antibody in the first auxiliary agent to obtain a serum amyloid A antibody solution, in which the serum amyloid A antibody has a mass concentration of 0.4%;

[0105] 3) dissolving EDC in the second auxiliary agent to obtain a coupling agent solution, wherein, in the coupling agent solution, the mass concentration of EDC is 10 mg / L;

[0106] 4) Mix the microsphere solution, the serum amyloid A antibody solution and the coupling agent solution for 24 hours, the rotation speed is 20 rpm, ultrasonic dispersion, ultrasonic power 50%, the ultrasonic on time is 2 s, the ultrasonic off time is 6 s, and the total ultrasonic time is 30min, wherein...

Embodiment 3

[0110] This embodiment provides a microsphere reagent and its preparation method.

[0111] Preparation:

[0112] 1) Dissolving styrene microspheres in the first auxiliary agent to obtain a microsphere solution, wherein, in the microsphere solution, the mass concentration of the microspheres is 0.1%;

[0113] 2) dissolving the serum amyloid A antibody in the first auxiliary agent to obtain a serum amyloid A antibody solution, and in the serum amyloid A antibody solution, the mass concentration of the serum amyloid A antibody is 0.5%;

[0114] 3) dissolving EDC in the second auxiliary agent to obtain a coupling agent solution, wherein, in the coupling agent solution, the mass concentration of EDC is 3 mg / L;

[0115] 4) Mix the microsphere solution, the serum amyloid A antibody solution and the coupling agent solution for 20 hours, the rotation speed is 40rpm, ultrasonic dispersion, ultrasonic power 30%, the ultrasonic on time is 3s, the ultrasonic off time is 3s, the total ultr...

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Abstract

The invention provides a preparation method of a microsphere reagent, which comprises the following steps: 1) mixing a microsphere solution, a serum amyloid protein A antibody solution and a couplingagent solution, and carrying out ultrasonic dispersion; 2) adding a third aid into the solution dispersed in the step 1), mixing, and ultrasonically dispersing; and 3) adding a fourth auxiliary agentinto the solution dispersed in the step 2), and mixing to obtain the microsphere reagent. The microsphere reagent is prepared by adopting a one-step method, a microsphere solution, a serum amyloid protein A antibody solution and a coupling agent solution only need to be mixed according to a specific proportion, and then ultrasonic dispersion is utilized. Meanwhile, the conditions of all the stepsand all factors are matched for synergistic interaction, and a microsphere reagent system which is uniform and stable in dispersion is obtained. The method is simple in operation, is less limited by equipment, is large in single production capacity, effectively improves the production efficiency, and reduces the production cost.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a method for preparing a microsphere reagent. Background technique [0002] Serum amyloid A protein (SAA) is an acute phase protein secreted by the liver and can bind to plasma high-density lipoprotein (HDL). Clinical studies are now focusing on the SAA type during the acute response to inflammatory disease. [0003] SAA is a sensitive parameter. It begins to increase about 8 hours after the inflammatory reaction, and it exceeds the upper limit of the reference range earlier than C-reactive protein (CRP). However, the difference between the median value of CRP in normal people and the upper limit of the reference range, There are about 10x compared to only 5x in SAA. Thus, elevated SAA is more common than CRP in mild infections, eg, many viral infections. In infectious diseases, the absolute rise of SAA is higher than that of CRP, so SAA measurement, especially for "normal" an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/543G01N33/68
CPCG01N33/531G01N33/54313G01N33/68G01N2333/4709
Inventor 李子樵王森
Owner AILEX TECH GRP CO LTD
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