Extractive electrospray ionization mass spectrometry method for inside of tissue sample

Abstract

Disclosed is an extractive electrospray ionization mass spectrometry method for the inside of a tissue sample. An extractive electrospray device comprises a sample bracket, a micro-syringe and a quartz capillary tube, one end of the quartz capillary tube is connected with a sampling needle of the micro-syringe, the other end of the quartz capillary tube is directly inserted into the sample to enable the tail end of the quartz capillary tube to be horizontal, but the other end of the quartz capillary tube isn't inserted through the sample, and the tail end of the quartz capillary tube is kept coaxial to the front end of the sample and an inlet of a mass spectrometer, an extracting agent is contained in the micro-syringe, a sampling pump controls flow velocity to push the extracting agent into the sample, direct current voltage is added to the sampling needle of the micro-syringe to perform extraction, desorption and ionization to the sample and form spraying at the front end of the sample, and then the sample enters the mass spectrometer to be analyzed. The extractive electrospray ionization mass spectrometry method for the inside of the tissue sample is wide in application range and capable of directly and rapidly obtaining information of molecules in the sample, and according to experiments, the method is simple in device, low in manufacturing cost, high in ionization efficiency and small in damage to the sample.

Description

technical field [0001] The invention relates to a method for electrospray extraction ionization mass spectrometry inside tissue samples, in particular to a method for rapid and direct mass spectrometry analysis of unpretreated solid complex samples based on simple ionization device to realize ionization of samples. Background technique [0002] Mass spectrometry is a chemical analysis method with high sensitivity, high specificity and high throughput in some forms. It is an effective tool for analyzing complex mixtures and can obtain important molecular information such as the chemical structure of analytes. [0003] The mass spectrometry analysis of complex samples generally requires a cumbersome sample pretreatment process, so it is impossible to achieve rapid and direct analysis of samples. Traditional mass spectrometry techniques are generally used in conjunction with gas chromatography or liquid chromatography for the analysis of complex samples, such as GC-MS and LC-MS...

AI Technical Summary

Problems solved by technology

However, the analysis techniques listed above also have their own shortcomings. For example, MALDI-MS or ELDI-MS still has a certain degree of damage to the sample, and the high-energy lasers that these techniques rely on may decompose some easily decomposable substances in the sample and cannot detected

Images