Unlock instant, AI-driven research and patent intelligence for your innovation.

Detection kit and detection method for CFHR1 genotypes and CFHR1 on high-density lipoprotein and oxidation phosphorylcholine

A high-density lipoprotein and genotype technology, applied in the field of CFHR1 genotype, CFHR1 and oxidized phosphorylcholine on high-density lipoprotein assay kits and the field of determination, can solve problems such as the unknown mechanism of AMD risk impact

Active Publication Date: 2013-03-20
张康
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the mechanism by which it affects AMD risk is largely unknown

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection kit and detection method for CFHR1 genotypes and CFHR1 on high-density lipoprotein and oxidation phosphorylcholine
  • Detection kit and detection method for CFHR1 genotypes and CFHR1 on high-density lipoprotein and oxidation phosphorylcholine
  • Detection kit and detection method for CFHR1 genotypes and CFHR1 on high-density lipoprotein and oxidation phosphorylcholine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0133] Composition and use of embodiment one kit of the present invention

[0134] 1. Use of genotyping kit

[0135] a. DNA template extraction from human tissue samples

[0136] b. Preparation of peripheral blood genomic DNA as a template for determination;

[0137] The method for the preparation of the assay template is:

[0138] 10XNEB Buffer 1ul

[0139] dNTP(10uM) 0.25ul

[0140] Primer-F(10uM) 0.25ul

[0141] Primer-R (10uM) 0.25ul

[0142] NEB Taq (5U / ul) 0.05ul

[0143]DNA (25-50ng / ul)1-1.5ul

[0144] H2O supplemented to 10ul

[0145] PCR: 95 degrees for 5 minutes

[0146] 95 degrees 30s

[0147] 58 degrees 30s,

[0148] 72 degrees 30s 30-32cycle

[0149] 72 degrees 5min

[0150] Electrophoresis to verify the success of PCR.

[0151] The primer prepared by DNA template among the present invention is:

[0152] rs6677604-tagged CFHR1

[0153] Upstream primer: 5'-GATGGAAGACACCAGAGCAGAT-3' (SEQ ID No 1)

[0154] Downstream primer: 5'-GAATCCATCAGCTTCTACCATT...

Embodiment 2

[0212] Embodiment 2 Pathological experiments and results related to human AMD

[0213] 1. Immunohistochemistry of Human AMD Eyes

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention aims at solving the technical problem to provide a diagnostic reagent for occurrence and development of age-related macular degeneration (AMD) diseases, and relates to a kit and a detection method thereof for identifying CFHR1 genotypes, and detecting content of CFHR1 in plasma samples, combined quantity of the CFHR1 on high-density lipoprotein particles and combination of the CFHR1 with oxidation specific antigen. The kit and the detection method thereof provide reliable reference indicators for diagnosis of the occurrence and the development of the AMD diseases, and creatively verify different genotype CFHR1. The kit and the detection method thereof develop a new strategy for preventing and treating aged yellow spot degeneration through restraining of CFHR1 activity, or inflammation genes formed on participated choroids of the CFHR1, and restraining of IL-1 activity, expect to prevent and treat the aged yellow spot degeneration and have a wide development and application prospect.

Description

technical field [0001] The present invention relates to the genotype identification of complement factor H-related protein 1 (CFHR1), the determination method of CFHR1 in plasma, the separation and purification of high-density lipoprotein-like particles containing CFHR1, the binding of CFHR1 to oxidation-specific antigens, and high-density lipoprotein Detection of oxidized phospholipids on protein (HDL), and through the data obtained from the above detection, early warning diagnosis and treatment guidance for age-related macular degeneration. It specifically relates to a relevant quantitative detection kit and a detection method thereof. Background technique [0002] The pathogenesis of age-related macular degeneration (AMD) involves the interaction of individual genetic variation and environmental risk factors such as oxidative stress. Severe loss of vision in AMD patients is mostly due to choroidal neovascularization (CNV) hemorrhage and exudation, or wet AMD. AMD is the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/11C12Q1/68G01N33/68G01N33/53G01N33/543
Inventor 张康彼得.肖
Owner 张康