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New bacterial strain-proteus penneri CA8 for generating coenzyme Q10 and applications thereof

A proteus and coenzyme technology, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of large extraction solvent loss, cumbersome steps, time-consuming and laborious, etc., and achieve industrial production, high output, and simple extraction methods Effect

Active Publication Date: 2014-09-03
浙江中科宏寿医药科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The method of extracting coenzyme Q10 from fermented bacteria is mostly an extraction method after saponification of alcohol and alkali, the steps are cumbersome, time-consuming and laborious, and the loss of extraction solvent is large

Method used

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  • New bacterial strain-proteus penneri CA8 for generating coenzyme Q10 and applications thereof
  • New bacterial strain-proteus penneri CA8 for generating coenzyme Q10 and applications thereof
  • New bacterial strain-proteus penneri CA8 for generating coenzyme Q10 and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] Example: Isolation, Identification and Fermentation of Proteus penneri CA8 to Produce Coenzyme Q 10 feature

[0027] 1. Isolation of strains:

[0028] (1) Collect samples

[0029] After collecting soil samples from Ebolowa, Cameroon, take 10 g and add 90 ml of sterile water to make a soil suspension. Draw the supernatant and dilute with sterile water to make 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 、10 -6 different dilutions.

[0030] (2) Strain isolation

[0031] Take 1 ml of the bacterial suspension, and streak it on the selective plate. The selective medium is prepared according to the following composition: 0.5 ml of isoprene, (NH 4 ) 2 SO 4 1.0 g, KH 2 PO 4 4.5 g, Na 2 HPO 4 12H 2 O 21.6 g, agar 15-20 g, water 1000 ml, pH 7.0.

[0032] According to the conventional method for the isolation of pure microorganisms, the above isolation culture was placed at 28 °C for 2-3 days. Pick multiple single colonies, inoculate them on the slant medium, and save th...

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Abstract

The invention provides a new bacterial strain-proteus penneri CA8 for generating coenzyme Q10 and applications of the proteus penneri CA8 in preparation of the coenzyme Q10 by microbial fermentation. The bacterial strain is preserved in China Center for Type Culture Collection, the address is 430072, Wuhan University, Wuhan, China, the preservation number is CCTCC No: M2012208, and the preservation date is June 10, 2012. The new bacterial strain-proteus penneri CA8 for generating the coenzyme Q10 has the following main beneficial effects: the new coenzyme Q10 producing strain is provided, and the coenzyme Q10 is prepared by utilizing the microbial fermentation of the strain, so that yield is high, the subsequent extraction method is simple, and the industrial production is facilitated.

Description

(1) Technical field [0001] The invention relates to a strain producing coenzyme Q 10 A new strain—Proteus pennzii CA8 and its production of coenzyme Q by microbial fermentation 10 The application in the invention belongs to the field of microbial pharmacy. (2) Background technology [0002] coenzyme Q 10 (CoenzymeQ 10 , CoQ 10 ) widely exists in animals, plants and microorganisms, and is an important hydrogen transporter in the respiratory chain of biological cells, as well as a good biochemical drug. It has the functions of anti-oxidation, eliminating free radicals, and improving the body's immunity. It has been widely used in the treatment of various heart diseases, diabetes, cancer, acute and chronic hepatitis, Parkinson's disease and other diseases. Preparation of Coenzyme Q 10 The methods mainly include animal cell extraction method, tobacco leaf solanesol semi-synthesis method, complete synthesis method, microbial fermentation method or plant cell culture method....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/66C12R1/01
Inventor 钟卫鸿孔卓怡柳华贵钟莉邱乐泉吴石金
Owner 浙江中科宏寿医药科技有限公司
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