Micro-fluid cytometer and manufacture method thereof

A microfluidic and cytometer technology, applied in the field of medical detection, can solve the problems of cumbersome sample pretreatment process, bulky volume, and high mutual contamination rate, and achieve the effects of accurate and reliable detection results, low cost, and convenient portability and use.

Inactive Publication Date: 2013-03-27
ZHONGBEI UNIV
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Problems solved by technology

[0006] The present invention provides a micro-flow cytometer and a manufacturing method to solve the technical problems that most of the trad

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  • Micro-fluid cytometer and manufacture method thereof
  • Micro-fluid cytometer and manufacture method thereof
  • Micro-fluid cytometer and manufacture method thereof

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Embodiment Construction

[0059]A microfluidic cytometer, comprising a microfluidic chip, the microfluidic chip includes a bottom plate 12 made of polydimethylsiloxane and a cover made of polydimethylsiloxane matched with the bottom plate 12 sheet 13; the surface of the base plate 12 is provided with a microfluidic channel; the microfluidic channel includes a longitudinally arranged sample channel 2, and the two sides of the sample channel 2 are respectively provided with a sheath with the longitudinal centerline of the sample channel 2 as the axis of symmetry A flow reservoir 1; two sides of the sample channel 2 are respectively provided with a sheath flow channel 4 with the longitudinal centerline of the sample channel 2 as the axis of symmetry; each of the two sheath flow channels 4 passes through one end and the sheath on the same side The flow reservoir 1 is connected; the other ends of the two sheath flow channels 4 and the output end of the sample channel 2 are jointly connected with a focus dete...

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Abstract

The invention relate to the field of medical detection, particularly relates to a micro-fluid cytometer and a manufacture method thereof, and solves the technical problems that the traditional cytometer at present is huge in size, high in price and particularly high in mutual infection rate. The micro-fluid cytometer mainly comprises a micro-fluid chip and a specially-made external detection component, wherein a sample channel, a focusing detection channel and a sheath flow liquid storage tank are arranged on the micro-fluid chip; a sample preprocessing region and a buffer region for carrying out concentrating processing on to-be-detected cell sap are integrated in the sample channel; and a micro-column array is arranged in the sample preprocessing region. The manufacture method of the micro-fluid cytometer comprises the following steps of: manufacturing micro-fluid channels, and manufacturing and bonding a cover plate of the micro-fluid chip. According to the micro-fluid cytometer and the manufacture method thereof, the products are integrally microminiaturized, the cost is low, the concentrating processing on the to-be-detected cell sap in the chip can be realized, the micro-fluid cytometer is disposable, each sample is measured at one time, and the problem of interaction effects among the samples is completely avoided.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a microfluidic cytometer and a manufacturing method. Background technique [0002] Flow cytometry is a single cell quantitative analysis and sorting technique using flow cytometry. Flow cytometry (Flow Cytometry, FCM) is a high-tech technology developed in the 1970s. It integrates computer technology, laser technology, fluid mechanics, cytochemistry, and cellular immunology. It also has the ability to analyze and sort cells Features. It can not only measure cell size and internal particle properties, but also detect cell surface and cytoplasmic antigens, intracellular DNA, RNA content, etc. It is widely used in hematology, immunology, oncology, pharmacology, molecular biology and other disciplines . The structure of the traditional flow cytometer mainly includes a flow chamber, and a pair of sheath fluid tubes are symmetrically arranged on both sides of the flow chamber; one e...

Claims

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Application Information

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IPC IPC(8): G01N15/14
Inventor 王万军杨潞霞段俊萍张斌珍王春水郝晓剑张勇范新磊李向红邵国成
Owner ZHONGBEI UNIV
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