Micro-fluid cytometer and manufacture method thereof

Abstract

The invention relate to the field of medical detection, particularly relates to a micro-fluid cytometer and a manufacture method thereof, and solves the technical problems that the traditional cytometer at present is huge in size, high in price and particularly high in mutual infection rate. The micro-fluid cytometer mainly comprises a micro-fluid chip and a specially-made external detection component, wherein a sample channel, a focusing detection channel and a sheath flow liquid storage tank are arranged on the micro-fluid chip; a sample preprocessing region and a buffer region for carrying out concentrating processing on to-be-detected cell sap are integrated in the sample channel; and a micro-column array is arranged in the sample preprocessing region. The manufacture method of the micro-fluid cytometer comprises the following steps of: manufacturing micro-fluid channels, and manufacturing and bonding a cover plate of the micro-fluid chip. According to the micro-fluid cytometer and the manufacture method thereof, the products are integrally microminiaturized, the cost is low, the concentrating processing on the to-be-detected cell sap in the chip can be realized, the micro-fluid cytometer is disposable, each sample is measured at one time, and the problem of interaction effects among the samples is completely avoided.

Description

technical field [0001] The invention relates to the field of medical detection, in particular to a microfluidic cytometer and a manufacturing method. Background technique [0002] Flow cytometry is a single cell quantitative analysis and sorting technique using flow cytometry. Flow cytometry (Flow Cytometry, FCM) is a high-tech technology developed in the 1970s. It integrates computer technology, laser technology, fluid mechanics, cytochemistry, and cellular immunology. It also has the ability to analyze and sort cells Features. It can not only measure cell size and internal particle properties, but also detect cell surface and cytoplasmic antigens, intracellular DNA, RNA content, etc. It is widely used in hematology, immunology, oncology, pharmacology, molecular biology and other disciplines . The structure of the traditional flow cytometer mainly includes a flow chamber, and a pair of sheath fluid tubes are symmetrically arranged on both sides of the flow chamber; one e...

AI Technical Summary

Problems solved by technology

[0006] The present invention provides a micro-flow cytometer and a manufacturing method to solve the technical problems that most of the traditional blood cytometers are bulky, expensive, sample pretreatment process is cumbersome, and especially the cross-contamination rate is high.

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