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Application of mannitol in preparation of anti-stomach cancer drug

A mannitol and drug technology, which is applied in the application field of mannitol in the preparation of anti-gastric cancer drugs, can solve the problems that there is no clinical application precedent for mannitol anti-tumor therapy and the like.

Inactive Publication Date: 2014-04-09
广西壮族自治区肿瘤防治研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on whether mannitol can induce apoptosis of cancer cells at home and abroad, and there is no precedent of clinical application of mannitol for anti-tumor therapy

Method used

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  • Application of mannitol in preparation of anti-stomach cancer drug
  • Application of mannitol in preparation of anti-stomach cancer drug
  • Application of mannitol in preparation of anti-stomach cancer drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Basic experimental research on mannitol's anti-tumor effect (lung cancer)

[0036] 1 Experimental materials

[0037] 1.1 Materials

[0038] 1.1.1 Cell line: SPC-A-1 human lung cancer cell line was obtained from Shanghai Cancer Institute.

[0039] 1.1.2 Reagents: RPMI1640 and trypsin were purchased from Gibcol BRL; newborn bovine serum was purchased from Hangzhou Sijiqing Bioengineering Materials Co., Ltd.; sodium bicarbonate (analytical pure) was produced by Hebei Cizhou Pharmaceutical Factory; penicillin sodium and streptomycin sulfate It is produced by Harbin Pharmaceutical Group Pharmaceutical General Factory; Thiazolium blue (MTT) and dimethyl sulfoxide (DMSO) are products of Sigma; ATP kits (ATP extract, luciferin-luciferase, diluent, etc.) are produced by German DCS company Products; sodium citrate was produced by Guangzhou Chemical Reagent Factory; propidium iodide (PI) and RNaseA were products of Sigma; mannitol injection was purchased from Guangxi N...

Embodiment 2

[0058] Example 2 Study on the Apoptosis of Lung Adenocarcinoma Cells Induced by a Specific Concentration of Mannitol and Its Mechanism (Lung Adenocarcinoma)

[0059] 1 Materials and methods

[0060] 1.1 Main reagents and instruments: DMEM (high glucose) culture medium (subpackaged by American hyclone company Thermo Fisher Biochemical Beijing Co., Ltd.), trypsin, newborn calf serum (FBS purchased from Hangzhou Sijiqing Biological Co., Ltd. company), 20% mannitol (purchased from Anshuanghe Pharmaceutical Co., Ltd.), Giemsa staining kit (provided by the Testing Center of the First Affiliated Hospital of Guangxi Medical University), cell apoptosis hoechst33342 kit (purchased from Shanghai Biyuntian Bio Technology Co., Ltd.) Apoptosis Dapi staining kit, flow cytometry kit (both purchased from Nanjing Kaiji Biotechnology Development Co., Ltd.), Bcl-2 detection kit (purchased from Fuzhou Maixin Biotechnology Development Co., Ltd.) , Pentanediol, and transmission electron microscopy ...

Embodiment 3

[0103] Embodiment 3 breast cancer

[0104] Referring to Example 1, the MTT method was used to determine the cell inhibitory rate of a specific concentration of mannitol on breast cancer cells. The results showed that within 48 hours, mannitol could inhibit the proliferation of breast cancer cells in the concentration range of 1-150 μg / μl, and its inhibitory effect was enhanced with the increase of the concentration. Within 5 days, the inhibition rate decreased with the increase of concentration and time. Also increasing.

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Abstract

The invention discloses an application of mannitol in preparation of an anti-stomach cancer drug, and in particular relates to a use of a mannitol injection with a specific concentration of 10%-19% for treating stomach cancer. Traditionally, mannitol is only used as a diuretic, a high-permeability hypotensor and the like, while the inventor discovers through scientific experiments that mannitol has anti-tumor activity, and through several cell apoptosis detection technologies and by detecting apoptosis-related genes bcl-2, and bax, proves that the specific-concentration mannitol can induce various human cancer cell strains to generate obvious cancer cell apoptosis, grasps the effect relation with concentration and time, and first illustrates the possible mechanism; furthermore, the inventor has used the research result for tumor patient treatment and has achieved obvious curative effect, which provides theoretical basis and practical cases for the application of mannitol in the preparation of the anti-tumor drug and the clinical generalization thereof.

Description

technical field [0001] The invention relates to a new application of mannitol, especially the application of mannitol in the preparation of anti-gastric cancer drugs. Background technique [0002] At present, in anti-tumor research, inducing the apoptosis of cancer cells is an important way. Promoting tumor cell apoptosis can achieve the purpose of tumor shrinkage and cancer regression. For example, traditional anti-tumor drugs cisplatin and melphalan can all Stimulates the mitochondrial apoptotic pathway in cells, triggering apoptosis. This method has obvious advantages over the treatment method of killing tumor cells, because if anti-tumor drugs can induce the apoptosis of tumor cells in large quantities and activate their own programmed death process, it will avoid the release of tumor cells due to a large number of necrotic cells. Various side effects caused by intracellular substances can reduce the damage of chemotherapy to normal cells and prolong the survival of pat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/047A61P35/00
Inventor 黄鼎铭刘德森黎乐群黄亚铭张力图曹骥黄其春吴军岳惠芬李黄羿潘琪黎斌杨立黄亚芬茅乃权左传田谢彤潘泓黄耀元欧超梁新强崔英
Owner 广西壮族自治区肿瘤防治研究所
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