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Method for preparation of fibrin glue from cottonseed meal

A protein glue, cotton meal technology, used in the preparation of protein adhesives, animal glue or gelatin, adhesives, etc.

Active Publication Date: 2013-04-03
周至县天一固本堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]In the traditional process, the cotton meal is directly denatured by protein alkali, so that the cellulose, fat and other substances in the cotton meal will affect the viscosity of the protein adhesive. The process usually adopts the method of crushing cotton meal through a 200-mesh sieve to reduce the influence of cellulose and fat on the viscosity of protein glue, but mechanical crushing consumes a lot of energy and is low in efficiency

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] Crush the cotton meal through a 200-mesh sieve, add water, the mass ratio of cotton meal to water is 1:4, add 0.06% cellulase by dry weight of cotton meal, and lipase by 0.03% by dry weight of cotton meal for enzymatic hydrolysis. After inactivating the enzyme, add caustic soda with 6% dry weight of cottonseed meal, heat in a constant temperature water bath at 50°C for 30 minutes, stir and mix evenly to make protein glue.

Embodiment 2

[0012] Crush the cotton meal through a 200-mesh sieve, add water, the mass ratio of cotton meal to water is 1:4, add 0.07% cellulase by dry weight of cotton meal, and lipase by 0.04% by dry weight of cotton meal for enzymatic hydrolysis. After inactivating the enzyme, add caustic soda with 5% dry weight of cottonseed meal, heat in a constant temperature water bath at 50°C for 30 minutes, stir and mix evenly to make protein glue.

Embodiment 3

[0014] Crush the cotton meal through a 200-mesh sieve, add water, the mass ratio of cotton meal to water is 1:4, add 0.08% cellulase and 0.05% lipase by dry weight of cotton meal for enzymatic hydrolysis. After inactivating the enzyme, add caustic soda with 4% dry weight of cottonseed meal, heat in a constant temperature water bath at 50°C for 30 minutes, stir and mix evenly to make protein glue.

[0015] The viscosities of the protein glues prepared in the above three examples were measured respectively. At the same time, the above three kinds of protein glues were used to prepare plywood, and the tensile strength of the board was measured. The measured results are shown in Table 1.

[0016] Table 1. The viscosity of the protein glue and the tensile strength of the plate made

[0017] Tensile strength (MPa) Viscosity (PaS) Example 1 2.548 78.23 Example 2 2.632 78.65 Example 3 2.671 78.99

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PUM

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Abstract

The invention relates to a method for preparation of a fibrin glue from cottonseed meal. The method includes: crushing the cottonseed meal and sieving it through a 200-mesh sieve, adding water, controlling the cottonseed meal and water in a mass ratio of 1:4, adding cellulose and lipase to perform enzymolysis, after enzyme deactivation, adding caustic soda, carrying out constant temperature waterbath heating for 30min under 50DEG C, and stirring and mixing the mixture evenly, thus obtaining the fibrin glue. The fibrin glue prepared by the method is more delicate and has higher viscosity.

Description

technical field [0001] The invention relates to a method for preparing protein glue from cotton meal, which belongs to the field of plant extraction. technical background [0002] Cotton meal is obtained by pressing cottonseed and separating residual oil. Cotton meal contains more than 40% crude protein and about 20% crude fat and crude fiber. Cotton meal is usually used for feed, but the composition of crude protein in cotton meal is not balanced, and usually contains free gossypol, which is highly toxic to animals, so feed is not the most ideal use of cotton meal. The invention processes the protein in the cottonseed meal into protein glue, which can be used as an adhesive for artificial boards. [0003] In the traditional process, the cotton meal is directly denatured by protein alkali, so that the cellulose, fat and other substances in the cotton meal will affect the viscosity of the protein adhesive. The traditional process usually uses the method of crushing the cott...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09J189/00
Inventor 苏建丽
Owner 周至县天一固本堂生物科技有限公司