Manufacture method and detection method for raw milk total bacterial count test paper

Abstract

The invention relates to a manufacture method for raw milk total bacterial count test paper, and the manufacture method comprises the following steps of: drying filter paper; soaking the dried filter paper for the first time, wherein the soak solution adopted in the soaking process is a water solution of a surfactant and buffer salt, the PThe invention relates to a manufacture method for raw milk total bacterial count test paper, and the manufacture method comprises the following steps of: drying filter paper; soaking the dried filter paper for the first time, wherein the soak solution adopted in the soaking process is a water solution of a surfactant and buffer salt, the PH value of the water solution is 6.8-8.0, and the filter paper is soaked in the soak solution for 1-10 minutes; drying the filter paper soaked for the first time; soaking the dried filter paper for ten minutes for the second time, wherein the soak solution adopted in the soaking process is reaction solution mixed by resazurin solution and calcium sulfate solution, the mass percent of the resazurin solution is 0.1%-1%, the mass percent of the calcium sulfate solution is 0.8%-3.8%, and the volume ratio of the resazurin solution and calcium sulfate solution is (1:1)-(10:1); and drying the filter paper soaked for the second time again.H value of the water solution is 6.8-8.0, and the filter paper is soaked in the soak solution for 1-10 minutes; drying the filter paper soaked for the first time; soaking the dried filter paper for ten minutes for the second time, wherein the soak solution adopted in the soaking process is reaction solution mixed by resazurin solution and calcium sulfate solution, the mass percent of the resazurin solution is 0.1%-1%, the mass percent of the calcium sulfate solution is 0.8%-3.8%, and the volume ratio of the resazurin solution and calcium sulfate solution is (1:1)-(10:1); and drying the filter paper soaked for the second time again.

Description

Technical field [0001] The invention relates to the field of dairy product detection, in particular to a manufacturing method and a detection method of a test paper for detecting the total number of bacteria in raw milk. Background technique [0002] High-quality raw milk is the foundation for the development of the dairy industry. Raw milk is rich in nutrients and is a natural fine culture medium. Microorganisms can grow rapidly under suitable conditions, making raw milk deteriorate and cannot be consumed or processed. The determination of the total number of bacteria is an important indicator to measure the quality of raw milk, and has important guiding significance for the production and quality control of dairy products. At present, the methods for measuring the total number of bacteria are widely used in the industry, including the standard plate counting method (SPC method) and flow cytometry. These two methods have certain defects in practical applications. [0003] The ma...

AI Technical Summary

Problems solved by technology

[0003] The main defects of the SPC method include: ① SPC method has high requirements on the field conditions of milk collection, most of the domestic milk collection field conditions are simple, and the SPC method cannot be operated; Limiting the popularization and application of SPC method; ③The detection result of SPC method has a strong hysteresis, and cannot quickly reflect the total number of bacteria indicators in raw milk in the first time

[0004] The main disadvantages of flow cytometry include: high detection cost, the detection cost of each sample is about 5RMB, a large number of samples will have the problem of cost inversion

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