Application of cGP and derivatives in preparation of medicine for inhibiting tumor growth and regeneration
A tumor and drug technology, applied in the field of anti-tumor drugs
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Embodiment 1c
[0047] Example 1 cGP can inhibit the proliferation of vascular endothelial cells mediated by IGF-1
[0048] Experimental method: In order to illustrate the role of cGP in the proliferation of vascular endothelial cells mediated by IGF-1, human microvascular endothelial cells HMEC-1 were cultured with 100 nM IGF-1, IGF-1+cGP and irrelevant control respectively, respectively, at 4 , 6, 8 and 10 days for the total cell count, Control (control). Each set of data was repeated 3 times. In order to illustrate the effect of cGP on different doses of IGF-1, HMEC-1 cells were plated in a six-well plate at a density of 30,000 / well, and after 24 hours, they were treated with 0 (A), 10 (B) or 50 (C) nM IGF-1 and cGP were treated together, and the cells were counted after 48h, Control (control).
[0049] Experimental equipment: cell counter, cell incubator, etc.
[0050] Experimental conclusion Compared with the control group, IGF-1 can stimulate the proliferation of endothelial cells, s...
Embodiment 2c
[0052] Example 2 cGP can inhibit the formation of tube-like structures by vascular endothelial cells
[0053] Experimental method: Human microvascular endothelial cells HMEC-1 were treated with IGF-1, IGF-1+cGP and irrelevant control for 6 days, and then the cells were transferred to Matrigel for analysis. Each set of data was repeated 15 times.
[0054] Experimental equipment: cell incubator, inverted microscope, etc.
[0055] Experimental results: image 3 shows that in the figure ** p*** p<0.001, the total length of the tube-like structure formed by the cells in the IGF-1+cGP group was shorter than that in the IGF-1-only treatment group. Thus it was determined that cGP could significantly inhibit the formation of tube-like structures in vascular endothelial cells induced by IGF-1.
Embodiment 3
[0056] Example 3 cGP can inhibit the occurrence and further development of tumors in mice
[0057] Experimental method: In order to verify that cGP can prevent tumor growth, we obtained 12 mice (8 weeks old, male C57BL / c) from the University of Auckland. We randomly divided the mice into two groups, six in each group, called the control group and the cGP group, respectively. We anesthetized mice by intraperitoneal injection of ketamine or xylazine. Next, an incision of approximately 5-10 mm was made on the back of each mouse near the middle of the shoulder blade, thus forming a subcutaneous pocket. Next, we embedded a mini-implantable capsule osmotic pump filled with 100 μl of cGP peptide solution into this pocket, and closed the incision with wound clips. An osmotic pump delivers cGP drug at a dose of 25 mg / kg / day. However, since the mice were under anesthesia, EL-4 thymic lymphoma cells (2*10 5 cells). We performed cGP treatment directly after tumor implantation ( Fig...
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