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Chitosan nano particle, biological pesticide preparation for controlling cabbage moths and preparation method thereof

A technology of chitosan nanoparticles and biopesticides, applied in botany equipment and methods, biocides, animal repellents, etc., can solve the problems of small molecular weight, limitations, and unrealized standardization level, so as to improve stability, The effect of standardized production steps and high pest control effect

Active Publication Date: 2013-05-22
GUANGZHOU RIBOBIO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above reports have successfully prepared pesticide chitosan nanoparticle preparations, their research and development are still in the exploratory stage, and the physical and chemical indicators such as the size, particle size distribution, and drug loading rate of the prepared nanoparticles are unstable, and the standardization level has not been achieved. It is cumbersome and difficult to achieve large-scale production, so it has been limited to laboratory research so far, and has not been transformed into a product preparation circulation market
In addition, the active ingredients of the pesticide chitosan preparations successfully reported so far are mostly existing chemical pesticides with small molecular weight and relatively simple technical operation.

Method used

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  • Chitosan nano particle, biological pesticide preparation for controlling cabbage moths and preparation method thereof
  • Chitosan nano particle, biological pesticide preparation for controlling cabbage moths and preparation method thereof
  • Chitosan nano particle, biological pesticide preparation for controlling cabbage moths and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] This example particularly provides the preparation and effect research of the chitosan nanoparticle biological preparation with the double-stranded ribonucleic acid sequence of the mitochondrial complex IIIFe-S subunit gene of Plutella xylostella xylostella as an active ingredient.

[0045] (1) Design and synthesis of the active ingredient of dsRNA for the mitochondrial complex IIIFe-S subunit gene of diamondback moth

[0046] GeneBank accession number: EU815629

[0047] Si-UQCR_001: GCAAG TCCGT CACCT TCAA

[0048] Sense strand (5'-3'): 5'GCAAG UCCGU CACCU UCAA dTdT 3' SEQ ID NO.1

[0049] Antisense strand (3’-5’): 3’dTdT CGUUC AGGCA GUGGA AGUU 5’ SEQ ID NO.2

[0050] Si-UQCR_002: CATCC AGTGT AGTGA GCAA

[0051] Sense strand (5'-3'): 5'CAUCC AGUGU AGUGA GCAA dTdT 3' SEQ ID NO.3

[0052] Antisense strand (3’-5’): 3’dTdT GUAGG UCACA UCACU CGUU 5’ SEQ ID NO.4

[0053] Si-UQCR_003: CAACA ACCTC TGAGA AGTT

[0054] Sense strand (5'-3'): 5'CAACA ACCUC UGAGA AGUU dTdT 3' ...

Embodiment 2

[0077] This example particularly provides the preparation and effect research of the chitosan nanoparticle biological preparation targeting the double-stranded ribonucleic acid sequence of the Plutella xylostella ecdysone receptor gene as the active ingredient.

[0078] (a design and synthesis of the original drug composition for double-stranded RNA of the diamondback moth ecdysone receptor gene

[0079] GeneBank: EF417852

[0080] Si-EcR_001: CTCAC TCAAG CTCAA GAAC

[0081] Sense strand (5'-3'): 5'CUCAC UCAAG CUCAA GAAC dTdT 3' SEQ ID NO.7

[0082] Antisense strand (3’-5’): 3’dTdT GAGUG AGUUC GAGUU CUUG 5’ SEQ ID NO.8

[0083] Si-EcR_002: AGGCA CAAAG GGAGA AGGA

[0084]Sense strand (5'-3'): 5'AGGCA CAAAG GGAGA AGGA dTdT 3' SEQ ID NO.9

[0085] Antisense strand (3’-5’): 3’dTdT UCCGU GUUUC CCUCU UCCU 5’ SEQ ID NO.10

[0086] Si-EcR_003: CTGCA TGTAC TCGCT GAAT

[0087] Sense strand (5'-3'): 5'CUGCA UGUAC UCGCU GAAU dTdT 3' SEQ ID NO.11

[0088] Antisense strand (3'-5'): 3...

Embodiment 3

[0108] This example particularly provides the preparation and effect research of the chitosan nanoparticle biological preparation targeting the double-stranded ribonucleic acid sequence of the Plutella xylostella γ-aminobutyric acid receptor gene as the active ingredient.

[0109] (1) Design and synthesis of double-stranded RNA technical drug against Plutella xylostella γ-aminobutyric acid receptor gene

[0110] Si-GABA_001: GGGTC TATTA CCAGA AGTA

[0111] Sense strand (5'-3'): 5'GGGUC UAUUA CCAGA AGUA dTdT 3' SEQ ID NO.13

[0112] Antisense strand (3’-5’): 3’dTdT CCCAG AUAAU GGUCU UCAU 5’ SEQ ID NO.14

[0113] Si-GABA_002: CCATG TATGT GCTCT CTAT

[0114] Sense strand (5'-3'): 5'CCAUG UAUGU GCUCU CUAU dTdT 3' SEQ ID NO.15

[0115] Antisense strand (3’-5’): 3’dTdT GGUAC AUACA CGAGA GAUA 5’ SEQ ID NO.16

[0116] Si-GABA_003: GTGGA GGAGA CGAGG ATAA

[0117] Sense strand (5'-3'): 5'GUGGA GGAGA CGAGG AUAA dTdT 3' SEQ ID NO.17

[0118] Antisense strand (3’-5’): 3’dTdT CACCU CC...

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Abstract

The invention discloses a chitosan nano particle which comprises the following raw materials in percentage by weight: 0.5-5% of chitosan, 1-10% of cosolvent, 0-5% of antifreezing agent, 0.1-1% of surfactant, 0.1-0.5% of emulsifier, 40-60% of buffer solution and the balance of water. The invention also provides a biological pesticide preparation for controlling cabbage moths and a preparation method thereof. The biological pesticide preparation mainly comprises the following components in percentage by weight: (A) 0.05-15% of active component: a siRNA (small interfering ribonucleic acid) is designed and synthesized in accordance with a cabbage moth high-efficiency killing target gene and is a 15-50nt double-chain RNA; (B) 19.95-55% of solvent; and (C) 30-80% of chitosan nano particle. The chitosan nano particle ensures that the stability and the cabbage moth control effect of the biological pesticide preparation are better.

Description

technical field [0001] The invention belongs to the technical field of agricultural insecticides, and specifically relates to a biological pesticide preparation and a preparation method thereof, in particular to a chitosan nanoparticle, a double-stranded ribonucleic acid-containing biological pesticide preparation for controlling diamondback moth and a preparation method thereof. technical background [0002] Diamondback moth (Plutella xylostella), also known as small caterpillar, two-headed point, hanging silkworm, belongs to Lepidoptera Plutella, is a worldwide migratory pest, and its larvae mainly harm Brassicaceae plants such as cabbage, Chinese cabbage, rapeseed, mustard greens, and radish , bringing great losses to the production of important economic vegetables. Plutella xylostella larvae are responsible for more than 90% of plant losses in some regions and cost an average of $1 billion per year globally to control them. All the vegetable production areas of my count...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N25/10A01N43/90A01P7/04
Inventor 张必良
Owner GUANGZHOU RIBOBIO
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