Trichodermalongibrachiatum GF-10 and application thereof

A technology of Trichoderma longum and strains, applied in the directions of application, fungicides, fungi, etc., can solve the problem of no relevant reports on biological control, and achieve the effects of easy cultivation and preservation, strong tolerance, and reasonable technical route.

Inactive Publication Date: 2014-09-03
BIJIE COMPANY OF GUIZHOU TOBACCO +1
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  • Abstract
  • Description
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Problems solved by technology

And there is no relevant report on the biological control of the above two diseases by Trichoderma longibrachiata

Method used

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Experimental program
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Embodiment Construction

[0028] (1) Tested strain: black shank of tobacco - Phytophthora nicotiana ( Phytophthora nicotianae Gzufp-25), Tobacco Alternaria Alternaria ( Aiternaria alternate Gzufc-18) was isolated in Bijie City and preserved in the Institute of Fungal Resources, College of Life Sciences, Guizhou University.

[0029](2) Isolation and purification of antagonistic bacteria: Accurately weigh 10.0g of tobacco plant root soil sample collected from Qianxi County, Bijie City, Guizhou Province, and use aseptic technique to add the soil sample to 90mL sterile water with glass Shake it for 10 minutes to make the soil sample evenly disperse into a dilution, then pipette 1mL of the soil dilution into 9mL of sterile water and mix well to form a soil suspension. Use the mixed bacteria method to inoculate, draw 1mL of the suspension into a sterilized petri dish with a diameter of 9cm, then pour the double-antibody Martin’s medium that has been melted and cooled to 45°C, mix well, and invert it afte...

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Abstract

The invention discloses a Trichoderma longibrachiatum strain (Trichodermallongibrachiatum GF-10) and its application. Its morphological characteristics: it grows rapidly on PDA, its back is colorless to yellow-green, and it can produce conidia when cultured at 28°C for 72 hours. Secondary branches of sporozoites are few, often protruding at right angles; phialides protrude directly from secondary branches, solitary, opposite, rarely whorled, gourd-shaped or flask-shaped, 5.3-11.6 μm long, wide 2.0-3.2 μm, conical shape of ultimate bottle stem; spores are gray-green or green, oval or oval, 3.4-6.6 μm long and 2.3-3.5 μm wide. The invention has strong tolerance to adverse environment, resistance to mutation, easy cultivation and preservation, and good effect of inhibiting tobacco black shank bacteria and tobacco red spot bacteria.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Trichoderma longibrachiata strain, and also relates to the use of the Trichoderma longibranchium strain in inhibiting tobacco black shank and tobacco red spot fungus. Background technique [0002] Tobacco black shank is the most important rhizome disease in tobacco leaf production, while tobacco red spot is an important leaf disease in tobacco leaf production. The control of both is particularly important for tobacco leaf production. [0003] At present, there are many control methods for tobacco black shank and tobacco red spot, including cultivating disease-resistant varieties, chemical control, crop rotation and biological control. Planting disease-resistant varieties is the most economical and effective way to prevent and control the two diseases. However, when using disease-resistant varieties to prevent and control the two diseases, not only the resistance level and stability...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 葛永怡李祝张传平庄培林黄化刚黄青明申燕孙希文刘阳
Owner BIJIE COMPANY OF GUIZHOU TOBACCO
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