Preparation method of HMG (Human Menopausal Gonadotropin) coarse product

A technology for crude product and urine is applied in the field of preparation of HMG crude product to achieve the effects of cost saving, simplified steps and simple steps

Active Publication Date: 2013-06-19
山东滨渤生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The kaolin used in the above method is expensive, so the production cost of HMG is higher. In the above method, pearl salt is sometimes used as a filter aid, and its cost is also higher; in addition, its disadvantages of the above method are: , the cost of the filter press is higher

Method used

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  • Preparation method of HMG (Human Menopausal Gonadotropin) coarse product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation method of HMG crude product comprises the following steps:

[0031] a. Collect the urine of menopausal women. In the urine of menopausal women, HIV, hepatitis B virus HBs-Ab, HBc-Ab, and Hbe-Ab were not detected, and filtered through a 40-mesh filter with 50% acetic acid Adjust the pH value to 3.8, and remove the precipitate after standing for 4 hours;

[0032] b. Add silica gel to the urine after removing precipitation in step a. The amount of silica gel added is 1% of the weight of the urine. Stir for 2 hours and let it stand for 1 hour. Wash the used silica gel with pure water for the above silica gel. to pH 7, then soak in 6mol / L hydrochloric acid for 2 hours, and finally wash the silica gel with pure water until the silica gel has a pH value of 5, and acidify the silica gel. When this pH value is reached, the silica gel has the strongest adsorption capacity;

[0033] c. Take the supernatant and dry the precipitate;

[0034] d. Place the precipitat...

Embodiment 2

[0045] The preparation method of HMG crude product comprises the following steps:

[0046] a. Collect the urine of menopausal women. In the urine of menopausal women, HIV, hepatitis B virus HBs-Ab, HBc-Ab, and Hbe-Ab were not detected, and filtered through a 40-mesh filter with 50% acetic acid Adjust the pH value to 3.9, and remove the precipitate after standing for 4 hours;

[0047] b. add silica gel to the urine after removing precipitation in step a, the addition of described silica gel is 1% of urine weight, stir for 2 hours, let stand for 1 hour, above silica gel is treated silica gel, its The treatment steps are: wash the silica gel with pure water to pH 7, then soak it in 6mol / L hydrochloric acid for 2 hours, and finally wash the silica gel with pure water to pH 4;

[0048] c. Take the supernatant and dry the precipitate;

[0049] d. Place the precipitate in the elution column and elute with 50% acetone and 1 / 15 mol of anhydrous sodium acetate. The conditions for elut...

Embodiment 3

[0053] The preparation method of HMG crude product comprises the following steps:

[0054] a. Collect the urine of menopausal women. In the urine of menopausal women, HIV, hepatitis B virus HBs-Ab, HBc-Ab, and Hbe-Ab were not detected, and filtered through a 40-mesh filter with 50% acetic acid Adjust the pH value to 4.0, and remove the precipitate after standing for 4 hours;

[0055] b. add silica gel to the urine after removing precipitation in step a, the addition of described silica gel is 1% of urine weight, stir for 2 hours, let stand for 1 hour, above silica gel is treated silica gel, its The treatment steps are: wash the silica gel with pure water to pH 7, then soak it in 6mol / L hydrochloric acid for 2 hours, and finally wash the silica gel with pure water to pH 6;

[0056] c. Take the supernatant and dry the precipitate;

[0057] d. Place the precipitate in the elution column and elute with 50% acetone and 1 / 15 mol of anhydrous sodium acetate. The conditions for elut...

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Abstract

The invention belongs to the technical field of biomedicines, and in particular relates to a preparation method of an HMG (Human Menopausal Gonadotropin) coarse product. The preparation method comprises the following steps of: a, collecting urine of climacteric women, filtering, adjusting pH value by acetic acid, and standing and removing precipitate; b, adding silicon gel to the urine to stir and standing; c, taking supernate and drying the precipitate; d, placing the precipitate in an elution column and eluting by acetone and absolute sodium acetate; e, adding acetone to the elution liquid and standing for 8 hours; and f, taking out the supernate, re-recovering, washing the precipitate twice by absolute ethyl alcohol and vacuum drying to obtain the HMG coarse product. With the adoption of the method for preparing the HMG coarse product, compared with conventional preparation method of the HMGcoarse product, the cost is low. Kaolin and halite are not used, and a press is not adopted. And the cost is saved. The method is simple in step without the ultrafiltration step, so that the steps are simplified.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for preparing crude HMG. Background technique [0002] Human menopausal gonadotropin (human menopausal gonadotropin, HMG) is a substance extracted from the urine of menopausal women, containing FSH and LH, and its role in stimulating follicle development is mainly FSH. Human chorionic gonadotropin (human chorionic, gonadotropin, HGG) is a substance extracted from the urine of pregnant women, which can make mature follicles ovulate, or be used for luteal phase support therapy after HMG therapy. [0003] The existing preparation method of HMG is: take the urine of menopausal women, add kaolin into the urine, then use a filter press to press the cake, add ammonia water at the same time, extract the active ingredient liquid, and then enter the ultrafiltration machine for ultrafiltration. to 15% of the original volume, then add acetone solution to precipitat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/575
Inventor 刘爱红陈方山
Owner 山东滨渤生物制品有限公司
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