Preparation method of HMG (Human Menopausal Gonadotropin) coarse product

Abstract

The invention belongs to the technical field of biomedicines, and in particular relates to a preparation method of an HMG (Human Menopausal Gonadotropin) coarse product. The preparation method comprises the following steps of: a, collecting urine of climacteric women, filtering, adjusting pH value by acetic acid, and standing and removing precipitate; b, adding silicon gel to the urine to stir and standing; c, taking supernate and drying the precipitate; d, placing the precipitate in an elution column and eluting by acetone and absolute sodium acetate; e, adding acetone to the elution liquid and standing for 8 hours; and f, taking out the supernate, re-recovering, washing the precipitate twice by absolute ethyl alcohol and vacuum drying to obtain the HMG coarse product. With the adoption of the method for preparing the HMG coarse product, compared with conventional preparation method of the HMGcoarse product, the cost is low. Kaolin and halite are not used, and a press is not adopted. And the cost is saved. The method is simple in step without the ultrafiltration step, so that the steps are simplified.

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for preparing crude HMG. Background technique

[0002] Human menopausal gonadotropin (human menopausal gonadotropin, HMG) is a substance extracted from the urine of menopausal women, containing FSH and LH, and its role in stimulating follicle development is mainly FSH. Human chorionic gonadotropin (human chorionic, gonadotropin, HGG) is a substance extracted from the urine of pregnant women, which can make mature follicles ovulate, or be used for luteal phase support therapy after HMG therapy.

[0003] The existing preparation method of HMG is: take the urine of menopausal women, add kaolin into the urine, then use a filter press to press the cake, add ammonia water at the same time, extract the active ingredient liquid, and then enter the ultrafiltration machine for ultrafiltration. to 15% of the original volume, then add acetone solution to precipitat...

Images