Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of HMG (Human Menopausal Gonadotropin) coarse product

一种粗品、尿液的技术,应用在HMG粗品的制备领域,能够解决压滤机成本高、生产成本高、价格昂贵等问题,达到成本低、步骤简单、节约成本的效果

Active Publication Date: 2015-04-22
SHANDONG BINBO BIOLOGICAL PROD CO LTD
View PDF1 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The kaolin used in the above method is expensive, so the production cost of HMG is higher. In the above method, pearl salt is sometimes used as a filter aid, and its cost is also higher; in addition, its disadvantages of the above method are: , the cost of the filter press is higher

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of HMG (Human Menopausal Gonadotropin) coarse product

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The preparation method of HMG crude product comprises the following steps:

[0031] a. Collect the urine of menopausal women. In the urine of menopausal women, HIV, hepatitis B virus HBs-Ab, HBc-Ab, and Hbe-Ab were not detected, and filtered through a 40-mesh filter with 50% acetic acid Adjust the pH value to 3.8, and remove the precipitate after standing for 4 hours;

[0032] b. Add silica gel to the urine after removing precipitation in step a. The amount of silica gel added is 1% of the weight of the urine. Stir for 2 hours and let it stand for 1 hour. Wash the used silica gel with pure water for the above silica gel. to pH 7, then soak in 6mol / L hydrochloric acid for 2 hours, and finally wash the silica gel with pure water until the silica gel has a pH value of 5, and acidify the silica gel. When this pH value is reached, the silica gel has the strongest adsorption capacity;

[0033] c. Take the supernatant and dry the precipitate;

[0034] d. Place the precipitat...

Embodiment 2

[0044] The preparation method of HMG crude product comprises the following steps:

[0045] a. Collect the urine of menopausal women. In the urine of menopausal women, HIV, hepatitis B virus HBs-Ab, HBc-Ab, and Hbe-Ab were not detected, and filtered through a 40-mesh filter with 50% acetic acid Adjust the pH value to 3.9, and remove the precipitate after standing for 4 hours;

[0046] b. add silica gel to the urine after removing precipitation in step a, the addition of described silica gel is 1% of urine weight, stir for 2 hours, let stand for 1 hour, above silica gel is treated silica gel, its The treatment steps are: wash the silica gel with pure water to pH 7, then soak it in 6mol / L hydrochloric acid for 2 hours, and finally wash the silica gel with pure water to pH 4;

[0047] c. Take the supernatant and dry the precipitate;

[0048] d. Place the precipitate in the elution column and elute with 50% acetone and 1 / 15 mol of anhydrous sodium acetate. The conditions for elut...

Embodiment 3

[0052] The preparation method of HMG crude product comprises the following steps:

[0053] a. Collect the urine of menopausal women. In the urine of menopausal women, HIV, hepatitis B virus HBs-Ab, HBc-Ab, and Hbe-Ab were not detected, and filtered through a 40-mesh filter with 50% acetic acid Adjust the pH value to 4.0, and remove the precipitate after standing for 4 hours;

[0054] b. add silica gel to the urine after removing precipitation in step a, the addition of described silica gel is 1% of urine weight, stir for 2 hours, let stand for 1 hour, above silica gel is treated silica gel, its The treatment steps are: wash the silica gel with pure water to pH 7, then soak it in 6mol / L hydrochloric acid for 2 hours, and finally wash the silica gel with pure water to pH 6;

[0055] c. Take the supernatant and dry the precipitate;

[0056] d. Place the precipitate in the elution column and elute with 50% acetone and 1 / 15 mol of anhydrous sodium acetate. The conditions for elutio...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biomedicines, and in particular relates to a preparation method of an HMG (Human Menopausal Gonadotropin) coarse product. The preparation method comprises the following steps of: a, collecting urine of climacteric women, filtering, adjusting pH value by acetic acid, and standing and removing precipitate; b, adding silicon gel to the urine to stir and standing; c, taking supernate and drying the precipitate; d, placing the precipitate in an elution column and eluting by acetone and absolute sodium acetate; e, adding acetone to the elution liquid and standing for 8 hours; and f, taking out the supernate, re-recovering, washing the precipitate twice by absolute ethyl alcohol and vacuum drying to obtain the HMG coarse product. With the adoption of the method for preparing the HMG coarse product, compared with conventional preparation method of the HMGcoarse product, the cost is low. Kaolin and halite are not used, and a press is not adopted. And the cost is saved. The method is simple in step without the ultrafiltration step, so that the steps are simplified.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for preparing crude HMG. Background technique [0002] Human menopausal gonadotropin (human menopausal gonadotropin, HMG) is a substance extracted from the urine of menopausal women, containing FSH and LH, and its role in stimulating follicle development is mainly FSH. Human chorionic gonadotropin (human chorionic, gonadotropin, HGG) is a substance extracted from the urine of pregnant women, which can make mature follicles ovulate, or be used for luteal phase support therapy after HMG therapy. [0003] The existing preparation method of HMG is: take the urine of menopausal women, add kaolin into the urine, then use a filter press to press the cake, add ammonia water at the same time, extract the active ingredient liquid, and then enter the ultrafiltration machine for ultrafiltration. to 15% of the original volume, then add acetone solution to precipitat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/575
Inventor 刘爱红陈方山
Owner SHANDONG BINBO BIOLOGICAL PROD CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products