Preparation method of HMG (Human Menopausal Gonadotropin) coarse product
一种粗品、尿液的技术,应用在HMG粗品的制备领域,能够解决压滤机成本高、生产成本高、价格昂贵等问题,达到成本低、步骤简单、节约成本的效果
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Embodiment 1
[0030] The preparation method of HMG crude product comprises the following steps:
[0031] a. Collect the urine of menopausal women. In the urine of menopausal women, HIV, hepatitis B virus HBs-Ab, HBc-Ab, and Hbe-Ab were not detected, and filtered through a 40-mesh filter with 50% acetic acid Adjust the pH value to 3.8, and remove the precipitate after standing for 4 hours;
[0032] b. Add silica gel to the urine after removing precipitation in step a. The amount of silica gel added is 1% of the weight of the urine. Stir for 2 hours and let it stand for 1 hour. Wash the used silica gel with pure water for the above silica gel. to pH 7, then soak in 6mol / L hydrochloric acid for 2 hours, and finally wash the silica gel with pure water until the silica gel has a pH value of 5, and acidify the silica gel. When this pH value is reached, the silica gel has the strongest adsorption capacity;
[0033] c. Take the supernatant and dry the precipitate;
[0034] d. Place the precipitat...
Embodiment 2
[0044] The preparation method of HMG crude product comprises the following steps:
[0045] a. Collect the urine of menopausal women. In the urine of menopausal women, HIV, hepatitis B virus HBs-Ab, HBc-Ab, and Hbe-Ab were not detected, and filtered through a 40-mesh filter with 50% acetic acid Adjust the pH value to 3.9, and remove the precipitate after standing for 4 hours;
[0046] b. add silica gel to the urine after removing precipitation in step a, the addition of described silica gel is 1% of urine weight, stir for 2 hours, let stand for 1 hour, above silica gel is treated silica gel, its The treatment steps are: wash the silica gel with pure water to pH 7, then soak it in 6mol / L hydrochloric acid for 2 hours, and finally wash the silica gel with pure water to pH 4;
[0047] c. Take the supernatant and dry the precipitate;
[0048] d. Place the precipitate in the elution column and elute with 50% acetone and 1 / 15 mol of anhydrous sodium acetate. The conditions for elut...
Embodiment 3
[0052] The preparation method of HMG crude product comprises the following steps:
[0053] a. Collect the urine of menopausal women. In the urine of menopausal women, HIV, hepatitis B virus HBs-Ab, HBc-Ab, and Hbe-Ab were not detected, and filtered through a 40-mesh filter with 50% acetic acid Adjust the pH value to 4.0, and remove the precipitate after standing for 4 hours;
[0054] b. add silica gel to the urine after removing precipitation in step a, the addition of described silica gel is 1% of urine weight, stir for 2 hours, let stand for 1 hour, above silica gel is treated silica gel, its The treatment steps are: wash the silica gel with pure water to pH 7, then soak it in 6mol / L hydrochloric acid for 2 hours, and finally wash the silica gel with pure water to pH 6;
[0055] c. Take the supernatant and dry the precipitate;
[0056] d. Place the precipitate in the elution column and elute with 50% acetone and 1 / 15 mol of anhydrous sodium acetate. The conditions for elutio...
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