Preparation and application of human apoptosis factor quantitative antibody chip

Abstract

The invention relates to a human apoptosis factor quantitative antibody chip and a kit. A buffer liquid capable of being mixed with various standard proteins is prepared; a protein extraction reagent capable of effectively extracting proteins and ensuring that the proteins keep excellent activity is prepared; and whether a color indicator is added to the antibody so as to prevent errors of missing addition or less addition in detection is detected. The method for preparing the human apoptosis factor quantitative antibody chip specifically comprises design of the antibody chip, processing of a glass slide, chemical modification of the glass slide, quality examination of the antibody, determination of optimal concentration of capture antibodies and detection antibodies, micro-array spotting of the antibody chip, sealing of the antibody chip, mounting of a multi-sample fence, quality control of the antibody chip, and packaging and putting in storage. The application of the human apoptosis factor quantitative antibody chip kit is oriented to detect the expression condition of the human apoptosis factor in diseases. The human apoptosis factor quantitative antibody chip provided by the invention is higher in detection flux, better in flexibility, smaller in detection background, higher in specificity, more scientific in operation and more convenient for detection.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to the preparation and application of a human cell apoptosis factor quantitative antibody chip. Background technique [0002] Apoptosis is a genetically programmed cellular event that plays an important role in normal tissue development and homeostasis. Defects in the apoptotic program are thought to lead to a variety of human diseases ranging from neurodegenerative and autoimmune diseases to tumors. disease. As a genetic process, apoptosis, like any other developmental process, can be disrupted by mutation, and alterations in the apoptotic pathway are believed to play a critical role in many disease processes, including cancer. For example, the disorder of apoptosis can lead to autoimmune diseases, viral infections, AIDS, cardiovascular diseases, osteoporosis, tumors, neurodegenerative diseases (Parkinson's disease), etc. Research into cancer initiation and progression has tradition...

AI Technical Summary

Problems solved by technology

The existing antibody chip fails to solve the key links such as spotting conditions, carrier treatment and modification, antibody immobilization, and sample processing in the production process of the antibody chip. Therefore, the throughput of product detection is small, the product is unstable, and the specificity is not high. And the reliability is not strong, and because of the small amount of coverage, it is easy to produce false positives or false negatives

The protein extraction solution provided in the antibody chip kit cannot maintain the activity of the protein well, and the extraction rate is low

The detection methods and reagents provided in the antibody kit also have problems such as poor specificity and high background of the experimental results

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