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Application of Oroxylin A

A technology of melaleuca paper and liver regeneration, which is applied in the field of biomedicine and can solve problems such as unclear effects of liver cells

Active Publication Date: 2013-07-24
HOSPITAL AFFILIATED TO GUANDONG MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the effect of Oroxylin A on liver cells is not yet clear

Method used

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  • Application of Oroxylin A
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  • Application of Oroxylin A

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Effect of Oroxylin A on mouse serum AST, ALT and Albumin content

[0030] 1. Establishment and administration of acute liver injury mouse model

[0031] The experimental animals used in this experiment were 8-week-old C57BL / 6 male mice (purchased from Shanghai Slack Experimental Animal Center), and CCl was injected intraperitoneally at 1 mL / Kg body weight 4 (Sigma) solution (CCl 4 1:3 miscibility with corn oil) to establish a mouse model of acute liver injury.

[0032] Oroxylin A (Sigma) was dissolved in 0.5% carboxymethylcellulose sodium (CMC-Na, Sigma) aqueous solution to a final concentration of 200 mg / mL, and injected with CCl 4 After 1h, according to the dose of 60mg / Kg body weight orally administered, control group mice were intragastrically administered the same volume of CMC-Na at the same time. Daily injection of CCl 4 Solution and oral administration of Oroxylin A solution 1 time, continuous treatment for 4 days.

[0033] 2. Serological tests

...

Embodiment 2

[0036] Example 2 The effect of Oroxylin A on the morphology of liver cells in mice with liver injury

[0037] 1. Establishment and administration of acute liver injury mouse model

[0038] The experimental animals used in this experiment were 8-week-old C57BL / 6 male mice (purchased from Shanghai Slack Experimental Animal Center), and CCl was injected intraperitoneally at 1 mL / Kg body weight 4 (Sigma) solution (CCl 4 1:3 miscibility with corn oil) to establish a mouse model of acute liver injury.

[0039] Oroxylin A (Sigma) was dissolved in 0.5% carboxymethylcellulose sodium (CMC-Na, Sigma) aqueous solution to a final concentration of 200 mg / mL, and injected with CCl 4 After 1 hour, the dose of 60mg / Kg body weight was administered by intragastric administration, and the mice in the control group were intragastrically administered the same volume of CMC-Na at the same time. Daily injection of CCl 4 Solution and oral administration of Oroxylin A solution 1 time, continuous tr...

Embodiment 3

[0043] Example 3 Effect of Oroxylin A on Hepatocyte Proliferation

[0044] Proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase δ. Because it only exists in the nucleus of proliferating cells, it is a good indicator to reflect the status of cell proliferation. This test passed the PCNA + Cell staining was used to test the effect of Oroxylin A on the proliferation ability of hepatocytes in mice with liver injury.

[0045] 1. Establishment and administration of acute liver injury mouse model

[0046] The experimental animals used in this experiment were 8-week-old C57BL / 6 male mice, which were intraperitoneally injected with 1 mL / Kg body weight of CCl 4 Solution (CCl 4 1:3 miscibility with corn oil) to establish a mouse model of acute liver injury.

[0047] Oroxylin A was dissolved in 0.5% carboxymethylcellulose sodium (CMC-Na) aqueous solution to a final concentration of 200 mg / mL, and injected with CCl 4 After 1 hour, the dose of 60mg / Kg bod...

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Abstract

The invention discloses application of Oroxylin A in preparation of medicines for promoting liver regeneration. Experiments show that Oroxylin A can effectively protect liver, improve liver regeneration by means of improving expression level of IL-1Ra and TNF-alpha of liver cells, and promote the reshaping of the liver structure and is beneficial to restore of liver function. Experiments show that the survival rate of mice with liver injury in experimental group can be remarkably improved by Oroxylin A.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to the application of melaleucain A, in particular to the application of melaleucain A in the preparation of medicines for promoting liver regeneration. Background technique [0002] The liver is an important organ that regulates the body's metabolism. Liver injury refers to liver cell death, inflammatory response, and tissue necrosis in varying degrees under the action of external physical or chemical factors. It is a common clinical serious hazard to human beings. Healthy disease. [0003] Liver injury is a pathological state shared by many liver diseases, and its cure rate is low. Its long-term existence is often an important cause of liver fibrosis, liver cirrhosis and liver cancer. It is of great clinical significance to develop drugs that can effectively promote liver regeneration and treat liver injury. [0004] Scutellaria baicalensis is a traditional Chinese medicine with antipyre...

Claims

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Application Information

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IPC IPC(8): A61K31/352A61P1/16
Inventor 朱润芝李明意刘洁曾国芳刘斌张庆余夏娟
Owner HOSPITAL AFFILIATED TO GUANDONG MEDICAL COLLEGE
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