Determination method for 2,4-dinitrophenol in dry food packaging paper

A technology of dinitrophenol and food wrapping paper, which can be applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as 2,4-dinitrophenol research, and achieve the effect of high sensitivity

Active Publication Date: 2013-08-07
CHINA TOBACCO JIANGSU INDAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the existing literature reports focus on the reports on wastewater, environmental samples, etc., and there is no research on the residual 2,4-dinitrophenol in paper.

Method used

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  • Determination method for 2,4-dinitrophenol in dry food packaging paper
  • Determination method for 2,4-dinitrophenol in dry food packaging paper
  • Determination method for 2,4-dinitrophenol in dry food packaging paper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for the determination of 2,4-dinitrophenol in dry food packaging paper, the specific process is as follows:

[0026] (1) Preparation of standard working solution

[0027] Weigh 0.2 g of standard 2,4-dinitrophenol, first disperse and dissolve it with 10 mL of ethanol, and then use methanol as solvent to dilute it into a 100 mL volumetric flask to obtain a standard stock solution with a concentration of 2000 mg / L. Then accurately pipette 5μL, 25μL, 100μL, 250μL, 500μL and 1000μL standard stock solutions, and dilute to a 100mL volumetric flask with methanol solvent. The concentrations of the series of standard working solutions are 0.1mg / L, 0.5mg / L, 2.0mg / L, 5.0mg / L, 10mg / L and 20mg / L respectively. The standard working solution needs to be prepared immediately.

[0028] (2) Preparation of sample solution

[0029] Weigh 0.5g of paper sample (accurate to 0.1 mg), cut it into pieces below 5mm×5mm, put it in a 50mL Erlenmeyer flask with a stopper, accurately pipett...

Embodiment 2

[0040] Embodiment 2 Qualitative confirmation of the target object 2,4-dinitrophenol

[0041] The qualitative confirmation method for the target 2,4-dinitrophenol must meet the following two conditions: ①Compared with the retention time of the standard sample chromatogram, the retention time of the sample chromatogram is within the allowable error of ±2.5%; ②Compared with the standard sample chromatogram. Spectral comparison of samples ( image 3 Its spectrogram, it can be seen from the figure that there is a strong ultraviolet absorption at a wavelength of 258nm, and the analysis wavelength is 258nm, and the spectrogram of the sample must be consistent.

Embodiment 3

[0042] Example 3 Mobile phase ratio optimization

[0043] This embodiment optimizes the proportion of the mobile phase used in the present invention, and the specific operation steps refer to Example 1:

[0044] The present invention optimizes the composition and ratio of the mobile phase, and the specific results are shown in Table 1. For different dry food wrapping papers, the matrix changes are quite different, and the peak of the target substance is too early to be interfered by the impurity peak brought by the sample matrix. Therefore, the time of the peak of the target substance should be extended as much as possible. For this reason, considering the peak eluting time (retention time) of the target substance, as well as the response of the peak area and peak height, methanol / 1% acetic acid (volume ratio V / V is 45 / 55) as the mobile phase is the better detection condition.

[0045] Table 1 The composition and proportion optimization test results of mobile phase

[0046] ...

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Abstract

The present invention discloses a determination method for 2,4-dinitrophenol in dry food packaging paper. The determination method comprises: standard working solution preparation, sample solution preparation, high-performance liquid chromatography analysis, standard curve drawing and result calculation. According to the determination method, extraction is performed on dry food packaging paper to obtain 2,4-dinitrophenol, a DAD detector is adopted to carry out qualitative confirmation and quantitative detection on the 2,4-dinitrophenol, operation is simple and accurate, the target object and other impurity chromatographic peaks brought by the sample matrix can be well separated with the used chromatographic conditions, good linear correlation is provided, a detection limit is 0.44 mg / kg, an average relative standard deviation is 1.6%, a standard addition recovery rate is 93.20-116.62%, and characteristics of rapidness, accuracy, high sensitivity, good repeatability, high recovery rate and the like are provided. The determination method is especially suitable for determination of 2,4-dinitrophenol content in dry food packaging paper.

Description

technical field [0001] The invention belongs to the technical field of detection of physical and chemical indicators of packaging materials, and in particular relates to a method for determining 2,4-dinitrophenol in dry food packaging paper (mainly including instant food packaging paper, candy paper, pasta paper, etc.). Background technique [0002] 2,4-Dinitrophenol (2,4-dinitrophenol, referred to as DNP), slightly soluble in water, soluble in organic solvents such as ethanol, ether, benzene and chloroform. 2,4-Dinitrophenol belongs to protoplasmic poison and is highly toxic. The lowest oral lethal dose (LDLo) for humans is 36 mg / kg, and the half-lethal dose (LD50) for rats is 30 mg / kg. The main mechanism of toxicity is direct Act on energy metabolism, stimulate oxidation process, inhibit phosphorylation process. Mainly used in industries such as dyes, developers, drugs, indicators, pesticides and wood preservation. [0003] Dry food packaging paper is an indispensable an...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 廖惠云朱龙杰庄亚东张映熊晓敏曹毅韩开冬张媛
Owner CHINA TOBACCO JIANGSU INDAL
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