A kind of method utilizing dendrobium officinale dregs to ferment Ganoderma lucidum in solid state

A technology of solid fermentation and dendrobium residue, which is applied in the field of microbial fermentation, can solve the problems of no research reports and achieve the effect of comprehensive utilization

Active Publication Date: 2014-10-08
SHAOXING RULIN BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] During the deep processing of Dendrobium candidum products, some fibrous residues will inevitably be produced. How to realize the efficient utilization of these processing wastes is an important component of the deep comprehensive utilization of Dendrobium candidum, but there is no research on this aspect at present to report

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Slant culture: inoculate the ganoderma lucidum strains on the slant medium (i.e. PDA medium), and cultivate it at 30°C for about 7 days until the mycelium covers the slant;

[0023] (2) Liquid seed culture, the slant bacteria were inoculated into a 250-milliliter Erlenmeyer flask equipped with 80 mL of seed medium, the composition of the seed medium (unit gram / liter): glucose 20, corn flour 20, bran 10, K H 2 PO 4 1. MgSO 4 ·7H 2 O2, natural pH, sterilized at 121°C for 20 minutes, cultured at 30°C for 7 days on a shaker with a rotation speed of 150 rpm;

[0024] (3) For solid fermentation culture, 10 mL of cultured seeds were inoculated into a 500 mL Erlenmeyer flask containing 100 g (dry material weight) of fermentation medium. Composition of fermentation medium: dry material 100g (Dendrobium slag 98g, bran 1.9g, KH 2 PO 4 0.1g), feed-to-water weight ratio 1:1, sterilized at 121°C for 30 minutes; cultured at 22°C for 10 days, the polysaccharide content was ...

Embodiment 2

[0032] Fermentation medium material water weight ratio 1: 1.2, sterilized at 121°C for 30 minutes; cultivated at 25°C for 10 days, other conditions were the same as Example 1, the polysaccharide content measured at the end of fermentation was 1.85%, and the ganoderma acid content was 0.472%.

Embodiment 3

[0034] Fermentation medium material water weight ratio 1: 1.2, sterilized at 121°C for 30 minutes; cultivated at 28°C for 15 days, other conditions were the same as in example 1, the content of mycelia polysaccharide measured at the end of fermentation was 2.75%, and the content of ganoderma acid was 0.518 %.

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Abstract

The invention belongs to the field of microbial fermentation, and discloses a method for fermenting ganoderma lucidum by using dendrobium officinale residue as a solid. The method of the present invention takes dendrobium slag as substrate, carries out the solid fermentation of Ganoderma lucidum, mainly realizes through the following steps: (1) slant cultivation, (2) seed cultivation, (3) solid fermentation cultivation, the cultivated seed is inoculated by 10% The dry material in the fermentation medium is composed of: Dendrobium slag 98%, bran 1.9%, KH2PO 40.1%, dry material and water weight ratio 1:1-2.3, pH natural, 22 Culture at -32°C for 7-20 days. The Dendrobium slag of the present invention is fermented and biotransformed by Ganoderma lucidum, the cellulose in it is decomposed and utilized by Ganoderma lucidum, and high-efficiency bioactive substances such as Ganoderma polysaccharides and Ganoderma acid are produced; the wastes of deep processing of Dendrobium products are converted into high value-added products , realizing the all-round comprehensive utilization of dendrobium raw materials.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for solidly fermenting ganoderma lucidum by using Dendrobium officinale residue. Background technique [0002] Dendrobium, also known as Dendrobium orchid, is mainly distributed in tropical and subtropical Asia, Australia and Pacific Islands, and there are more than 1,000 species in the world. Among the 76 species of Dendrobium in China, nearly 40 are used for medicinal purposes. Among them, Dendrobium candidum is the best among Dendrobium, and is listed as "the first of the nine Chinese immortal grasses". The tender stems of Dendrobium candidum are processed, twisted into spiral or spring shape, and dried in the sun, which is called Tiepi Fengdou. [0003] In recent years, with the rise of green consumption, especially people's attention to the safety of artificially synthesized chemicals, natural products have been paid more and more attention in the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04C05F5/00C05G1/00
CPCY02A40/20
Inventor 陈乃伟丁重阳
Owner SHAOXING RULIN BIOTECH
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