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Preparation method of multi-component nanometer medicine with collaborative treatment effect and high drug loading

A nano-drug, high drug-loading technology, applied in the field of multi-component nano-drug preparation, can solve the problems of low drug loading, in vivo degradation of carrier toxicity and side effects, complex preparation process of nano-carriers, etc. Effects of drug resistance, avoidance of biotoxicity and metabolic problems in vivo

Inactive Publication Date: 2013-08-14
SUZHOU UNIV
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  • Claims
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Problems solved by technology

This method improves the chemotherapeutic effect of the drug to a certain extent, but there are also some limitations, such as low drug loading, complex preparation process of nanocarriers, and potential toxic side effects and in vivo degradation of the carrier.

Method used

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  • Preparation method of multi-component nanometer medicine with collaborative treatment effect and high drug loading
  • Preparation method of multi-component nanometer medicine with collaborative treatment effect and high drug loading
  • Preparation method of multi-component nanometer medicine with collaborative treatment effect and high drug loading

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Embodiment Construction

[0023] The present invention will be described in detail below with reference to the accompanying drawings and in combination with embodiments.

[0024] refer to figure 1 Shown, there is the preparation method of the multi-component nanomedicine of synergistic therapeutic effect and high drug load, comprises the following steps:

[0025] Step 1) Dissolve three hydrophobic anticancer drugs, methotrexate, 10-hydroxycamptothecin and paclitaxel, at a mass ratio of 1:1:1 in dimethyl sulfoxide, and sonicate for 5 minutes to form a concentration of 3 mg / mL mixed solution;

[0026] Step 2) Take the mixed solution with a volume of 400 μL and add it dropwise into ultrapure water, and stir magnetically to obtain a multi-component nanomedicine suspension;

[0027] Step 3) Add polymaleic anhydride 18 carbene-polyethylene glycol with a concentration of 1 mg / mL and a volume of 300 μL to the multi-component nano drug suspension, ultrasonically disperse for 5 minutes, and let stand for 3 h...

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Abstract

The invention discloses a preparation method of a multi-component nanometer medicine with a collaborative treatment effect and high drug loading. The preparation method comprises the following steps of: 1) dissolving three hydrophobic cancer-fighting medicines, namely, methotrexate, 10-hydroxycamptothecine and taxol which are in a mass ratio of 1: 1: 1, into dimethylsulfoxide; ultrasonically treating for 5 minutes to produce mixed solution with concentration of 3mg / mL; 2) dropwise adding 400 micro-millimeters of the mixed solution into ultrapure water; and magnetically stirring to obtain suspension liquid of the multi-component nanometer medicine; and 3) adding 300 micro-millimetres of polymaleic anhydride 18 carbene-polyethylene glycol with concentration of 1mg / mL to the suspension liquid of the multi-component nanometer medicine; ultrasonically dispersing for 5 minutes; and standing for 3 minutes. The preparation method is simple, efficient, high in repeatability, excellent in controllability and high in universality, and provides experimental bases for preparing the nanometer medicine applied to multi-medicine collaborative treatment in the cancer field.

Description

technical field [0001] The invention relates to the field of biopharmaceuticals, in particular to a preparation method of a multi-component nano drug with synergistic therapeutic effect and high drug loading. Background technique [0002] In recent years, chemotherapy, as an effective tumor treatment method, has been clinically important, and multi-drug combination chemotherapy has shown great promise in the medical field due to its enhanced drug efficacy and inhibition of cell drug resistance. application potential. In 2010, Y. Q. Shen's group replaced fatty acids in phospholiposomes with a hydrophobic chemotherapy drug, camptothecin, to form a phospholiposome-like carrier structure, while loading another chemotherapy drug, doxorubicin hydrochloride. The resulting multi-drug nanocapsules exhibited better efficacy than the two single drugs, reflecting a synergistic therapeutic effect (Shen, Y. Q.; Jin, E. L.; Zhang. B.; Murphy, C. J.; Sui, M. H.; Zhao, J.; Wang, J. Q.; Tan...

Claims

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Application Information

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IPC IPC(8): A61K31/519A61K31/4745A61K31/337A61P35/00
Inventor 周梦娇
Owner SUZHOU UNIV
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