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Fluorescence contrast agent and preparation method thereof

A technology of fluorescent contrast agents and reagents, applied in the field of molecular biology, which can solve the problems of hidden tumor tissue removal and difficulty in directly observing tumors

Active Publication Date: 2015-01-07
SHENZHEN INST OF ADVANCED TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although near-infrared fluorescence imaging has a good effect, it is difficult to directly observe tumors, because the fluorescent contrast agent used in near-infrared fluorescence imaging needs to be excited with light in a dark room or under dark light to distinguish tumor cells
Because near-infrared fluorescence imaging is difficult to directly observe the tumor, it brings great inconvenience to the operator. The operator usually needs to distinguish tumor tissue from normal tissue under dark light, and there are great hidden dangers when removing tumor tissue.

Method used

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  • Fluorescence contrast agent and preparation method thereof
  • Fluorescence contrast agent and preparation method thereof
  • Fluorescence contrast agent and preparation method thereof

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preparation example Construction

[0036] This embodiment also provides a method for preparing a fluorescent contrast agent for tumor pathological diagnosis, such as figure 1 shown, including the following steps:

[0037] Step S110, mixing the chloroauric acid solution with a concentration of 1-100 mmol / L and the bovine serum albumin or human serum albumin solution with a concentration of 1-500 mg / mL evenly, and then adding a NaOH solution with a concentration of 0.1-10 mol / L , and incubated in a shaker at 0-100° C. for 3-100 hours to obtain gold nanoclusters, wherein the molar ratio of NaOH to chloroauric acid is 1:1-1:100.

[0038] In this embodiment, bovine serum albumin is preferred to prepare gold nanoclusters, because bovine serum albumin has the advantages of non-toxicity, good biocompatibility, and relatively cheap price.

[0039] Step S120 , mixing the gold nanoclusters with the azide reagent pretreated by NHS at a molar ratio of 1:1 to 1:100 to obtain gold nanoclusters with azide groups modified on t...

Embodiment 1

[0051] 1. Preparation of fluorescent contrast agent

[0052] 1. Preparation of gold nanoclusters: at 37°C, add 0.5mL of chloroauric acid solution with a concentration of 50mmol / L to 0.5mL of BSA solution with a concentration of 200mg / mL and mix well; then add 0.2mL with a concentration of 1mol / L of NaOH solution to obtain a mixed solution; the mixed solution was placed in a shaker and incubated at 37°C for 10 hours to obtain gold nanoclusters.

[0053] 2. Azido group modification of gold nanoclusters: the above gold nanoclusters and azide reagents are mixed according to the molar ratio of 1 to obtain gold nanoclusters with azide groups on the surface, wherein the azide reagents are pre-treated NHS dealt with.

[0054] 3. Alkynyl modification of anti-HER2 protein antibody: Add DBCO-sulfo-NHS Ester alkynylation reagent to anti-HER2 protein antibody and react for 30 minutes, wherein, DBCO-sulfo-NHS Ester alkynylation reagent and anti-HER2 protein antibody The molar ratio is 10...

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Abstract

The invention provides a fluorescence contrast agent. The fluorescence contrast agent comprises gold nanoclusters, antibodies of tumor specificity markers and coomassie brilliant blue, wherein the gold nanoclusters and the antibodies of the tumor specificity markers are connected in a click-chemistry mode; and the coomassie brilliant blue is combined with the gold nanoclusters connected with the antibodies of the tumor specificity markers. The fluorescence contrast agent provided by the invention has the characteristic of naked-eye visualization. In addition, the invention also provides a preparation method of the fluorescence contrast agent; and the method is simple in step and is suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a fluorescent contrast agent and a preparation method thereof. Background technique [0002] In the past two decades, the mortality rate of cancer has risen sharply. Among middle-aged and middle-aged people aged 35 to 59, tumors have ranked first among various causes of death. Statistics show that the incidence of cancer in my country is about 2 million per 100,000 people, with more than 2.2 million new cases each year and more than 6 million patients being treated. Tumor has become a major cause of the loss of the best labor force and the rise of medical expenses in our country. Although some chemical drugs to prevent tumors have been developed, there is still no primary preventive measure to effectively control tumors. Therefore, early diagnosis is still a basic strategy for tumor prevention and treatment at present and for a long time to come. [0003] Tumor diag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00
Inventor 蔡林涛胡德红盛宗海方胜涛王亚楠高笃阳龚萍张鹏飞
Owner SHENZHEN INST OF ADVANCED TECH
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