Method for comprehensively extracting effective ingredients in tea

A technology for comprehensive extraction of active ingredients, which is applied in the field of comprehensive extraction of active ingredients in tea and efficient preparation of active ingredients in tea, can solve problems such as extraction of tea protein, improve utilization rate and production efficiency, and make the production process efficient and environmentally friendly. The effect of reducing production costs

Active Publication Date: 2013-08-14
TIANJIN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

None of the above-mentioned patents considered the extraction of the same valuable tea protein in the tea dregs

Method used

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  • Method for comprehensively extracting effective ingredients in tea
  • Method for comprehensively extracting effective ingredients in tea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] (1) Weak acidic aqueous solution extraction of active ingredients of tea

[0033] Dedust and pulverize the dried tea leaves, and pass through a 60-80 mesh sieve. Take 10g of 60-80 mesh tea powder, add 150mL of phosphate buffer solution with a pH of 5, and then place the system in a constant temperature water bath with a temperature of 50°C to shake and extract. After extracting for 30 minutes, filter, collect tea dregs and dry them. Serum for later use.

[0034] (2) Extraction of tea protein

[0035]Take 5g of the tea residue collected in step (1), add 100mL of alkaline NaOH aqueous solution with a concentration of 0.06mol / L at a solid-to-liquid ratio of 1:20, and place the system in a constant temperature water bath at 70°C Shake and extract for 60 minutes, filter to obtain the alkaline water extract of tea protein; add 0.05mol / L hydrochloric acid to the alkaline water extract of tea protein, adjust the pH to 4.5 for precipitation, centrifuge to remove the supernatan...

Embodiment 2

[0044] (1) Weak acidic aqueous solution extraction of active ingredients of tea

[0045] Dedust and pulverize the dried tea leaves, and pass through a 60-80 mesh sieve. Take 10g of 60-80 mesh tea powder, add 250mL of phosphate buffer solution with a pH of 5-6, and then place the system in a constant temperature water bath with a temperature of 70°C to shake and extract. After extracting for 40 minutes, filter the tea dregs and add them according to the above steps 250mL of phosphate buffer solution with a pH of 5.5 was extracted once more, the tea dregs were collected by filtration and dried, and the supernatants of the two extractions were combined for later use.

[0046] (2) Extraction of tea protein

[0047] Take 5g of the tea residue collected in step (1), add 150mL of alkaline NaOH aqueous solution with a concentration of 0.08mol / L at a solid-to-liquid ratio of 1:30, and place the system in a constant temperature water bath at 80°C Shake and extract for 90 minutes, filt...

Embodiment 3

[0055] (1) Weak acidic aqueous solution extraction of active ingredients of tea

[0056] Dedust and pulverize the dried tea leaves, and pass through a 60-80 mesh sieve. Take 10g of 60-80 mesh tea powder, add 300mL of phosphate buffer solution with a pH of 5-6, and then place the system in a constant temperature water bath at 80°C to extract by shaking. The 6 phosphate buffer solution was repeatedly extracted twice, filtered, the tea residue was collected and dried, and the supernatants extracted three times were combined for later use.

[0057] (2) Extraction of tea protein

[0058] Take 5g of the tea dregs collected in step (1), add it into 200mL of alkaline NaOH aqueous solution with a concentration of 0.10mol / L at a solid-to-liquid ratio of 1:40, and place the system in a constant temperature water bath with a temperature of 90°C Shake and extract for 120 minutes, filter to obtain the alkaline water extract of tea protein; add 0.10mol / L hydrochloric acid to the alkaline w...

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Abstract

The invention relates to a method for comprehensively extracting effective ingredients in tea. The method comprises the following steps of: with a faintly acidic water solution as a main extraction solvent and a certain amount of organic solvent as an auxiliary extraction solvent, carrying out step-by-step extraction on water extracts to obtain four effective ingredients, namely tea polyphenol, caffeine, tea polysaccharide and theanine; and meanwhile, extracting tea protein from tea residues by using an alkali dissolution and acid deposition method. According to the method, as water is used as the main solvent, the cost is low, the reactions are mild and active ingredients are favorably preserved; as a certain amount of organic solvent is used as the auxiliary extraction solvent, the problems of overhigh cost and product pollution caused by use of a large amount of organic solvents are solved; meanwhile, five effective ingredients, namely the tea polyphenol, the tea polysaccharide, the caffeine, the theanine and the tea protein are extracted, and therefore high extraction rate, high purity and high utilization rate of tea are achieved; the solvents are recycled in the extraction process, so that the preparation cost is reduced; and no alkali and acid waste liquor is discharged in the overall process, and therefore the method is environment-friendly. The overall preparation flow is high in efficiency and low in cost, and therefore the method has a favorable application value.

Description

technical field [0001] The invention belongs to the technical field of tea deep processing, and relates to a method for comprehensively extracting active ingredients in tea, in particular to a method for efficiently preparing effective ingredients in tea suitable for industrial production. Background technique [0002] As a traditional health drink of the Chinese nation, tea has been loved and valued by people since ancient times. Not only that, in today's world, tea has become the first of the three major beverages (tea, coffee, cocoa). my country is a country rich in tea resources. However, as tea consumption becomes increasingly high-end, a large amount of middle and low-grade tea is unsalable. At the same time, a large number of by-products will be produced in the process of tea production and processing, and the utilization rate is extremely low. Therefore, using middle and low-grade tea and leftovers of tea processing to extract its active ingredients, vigorously deve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D473/12C07G99/00C07C237/06C07C231/24C07K1/14C08B37/00
Inventor 齐崴何志敏苏荣欣鹿洋王梦凡黄仁亮
Owner TIANJIN UNIV
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