Molecular fingerprint identification method of ferula ferulaeoides and congeneric sibling species

A multi-umbrella ferulae and identification method technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., to achieve the effect of saving time and cost

Inactive Publication Date: 2013-08-28
XINJIANG MEDICAL UNIV
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  • Abstract
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Problems solved by technology

[0007] The technical problem to be solved in the present invention is to overcome the defects of the prior art and provide a method for identifying

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  • Molecular fingerprint identification method of ferula ferulaeoides and congeneric sibling species
  • Molecular fingerprint identification method of ferula ferulaeoides and congeneric sibling species
  • Molecular fingerprint identification method of ferula ferulaeoides and congeneric sibling species

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Embodiment Construction

[0027] figure 1 It is a flow chart of an embodiment of the molecular fingerprint identification method of a kind of ferulicum ferulae and similar species of the same genus of the present invention, as figure 1 As shown, the method for identifying the molecular fingerprints of ferulae ferulae and similar species of the same genus in the present embodiment comprises the following steps:

[0028] 1. Sample collection:

[0029] The young leaves of Ferulicum ferulae and other samples of the same genus were collected from different places in Xinjiang, and the leaves were quickly dried in silica gel and stored at -80°C for future use.

[0030] 2. Three different total DNA extraction methods:

[0031] The young leaves of Ferulicum ferulae and the three kinds of medicinal plants of the same genus were taken, and the total DNA of the above four kinds of medicinal plants was extracted by kit method, improved CTAB method and SDS method; nucleic acid protein detector and agarose gel were...

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Abstract

The invention discloses a molecular fingerprint identification method of ferula ferulaeoides and congeneric sibling species, comprising the steps of creating a ferula ferulaeoides ISSR molecular marker optimization system, creating an ISSR (Inter Simple Sequence Repeat) fingerprint and identifying. The molecular fingerprint identification method specifically comprises the following steps: 1. collecting ferula ferulaeoides and other congeneric variety samples; 2. comparing genome DNA extraction methods of the samples; 3. optimizing ISSR-PCR (polymerase chain reaction) amplified reaction conditions and performing electrophoresis detection; 4. creating an ISSR molecular marker fingerprint; and 5. verifying the ferula ferulaeoides and the congeneric sibling species through the created DNA fingerprint. The molecular fingerprint identification method disclosed by the invention is beneficial to quickly and accurately identify ferula ferulaeoides varieties with external plant forms difficult to distinguish, saves time and cost and has important significance for accurately identifying medicinal material sources of ferula ferulaeoides.

Description

technical field [0001] The invention relates to a method for identifying molecular fingerprints of feruli multiforme and similar species of the same genus, including a method for constructing a ferulula multiforme molecular marker fingerprint, establishment of an ISSR molecular marker optimization system for feruli multiforme, and construction of an ISSR fingerprint. It also relates to a method for molecular identification of ferulicum ferulae and medicinal materials of the same genus and similar varieties, belonging to the field of identification of medicinal materials. Background technique [0002] There are more than 150 species of plants of the genus Ferula (Ferula L.) in the Umbelliferae family, mainly distributed in the Mediterranean region in southern Europe and northern Africa, as well as Iran, Afghanistan, Central Asia and Siberia of the Soviet Union, India, Pakistan, etc. land. There are 26 species and 1 variant in China, and 21 species are produced in Xinjiang. ...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 盛萍苗莉娟王飒安露莎
Owner XINJIANG MEDICAL UNIV
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