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ACE inhibitory peptide and preparation method thereof

A technology for inhibiting peptides and inhibiting activity is applied in the field of producing milk powder with anti-hypertensive effect, which can solve the problems of great influence on renal function, and achieve the effects of strong practicability and simple preparation method.

Inactive Publication Date: 2015-06-10
TIANJIN UNIV OF COMMERCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] During the onset of hypertension, patients need to take some auxiliary antihypertensive drugs frequently, but these antihypertensive drugs will produce many side effects, especially have a great impact on renal function

Method used

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  • ACE inhibitory peptide and preparation method thereof
  • ACE inhibitory peptide and preparation method thereof
  • ACE inhibitory peptide and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] 1. In the casein aqueous solution with a substrate concentration of 7%, add pepsin according to the ratio of enzyme to substrate of 1:100, the hydrolysis conditions of pepsin are: temperature is 37°C, pH value is 3.0, enzyme After 3 h of solution, the reaction was terminated by heating in a boiling water bath for 10 min, and cooled to room temperature.

[0066] 2. Adjust the pH value of the hydrolyzate obtained in step 1 to 7.7, and then continue the hydrolysis with trypsin. The conditions for the hydrolysis of trypsin are: the temperature is 48°C, the pH value is 7.7, and the ratio of trypsin to substrate is 1:500 ; After hydrolysis for 3 hours, the hydrolyzate was boiled in a boiling water bath for 10 minutes to inactivate the enzyme, and then cooled to 50°C. Adjust the pH of the hydrolyzate to 6.8, add flavor protease (Novo, Denmark) for debittering, the conditions are: the ratio of enzyme to substrate is 3200U / g, the hydrolysis temperature is 50°C, the pH is 6.8, an...

Embodiment 2

[0071] (1) In the casein aqueous solution with a substrate concentration of 7%, add pepsin according to the ratio of enzyme to substrate of 1:100, the hydrolysis conditions of pepsin are: temperature 37°C, pH value 3.0, After 3 hours of enzymatic hydrolysis, the reaction was terminated by heating in a boiling water bath for 10 minutes. Then, the hydrolyzate was cooled to 48°C. Adjust the pH value to 7.7, and then continue the hydrolysis with trypsin. The conditions for hydrolysis of trypsin are: temperature 48°C, pH value 7.7, ratio of trypsin to substrate 1:500; after 3 hours of hydrolysis, the hydrolyzate was boiled in a boiling water bath for 10 minutes to inactivate the enzyme, and then cooled to room temperature. Adjust the pH value of the hydrolyzate back to 7.0, centrifuge at 4000r / min, and take the supernatant. The supernatant was treated with an ultrafiltration membrane with a molecular weight cut-off of 6Ku, and the ultrafiltration permeate was collected. The ultr...

Embodiment 3

[0085] 1. The amino acid sequence obtained in Example 2 is that the ACE inhibitory peptide of YS and the ACE inhibitory peptide with the amino acid sequence of YQKFPQYLQY are mixed according to a 1:1 ratio, and then mixed with the milk powder matrix, and 0.6 grams are added in every 100 grams of milk powder matrix ACE inhibitory peptide, fully mixed evenly, to obtain milk powder with antihypertensive effect.

[0086] 2. The milk powder obtained in step 1 was given to essential hypertensive rats (SHR) orally, and its effect on the blood pressure of SHR was detected. Twenty-four 12-week-old male SHR rats with a body mass of (260±15) g were selected, fed and drank ad libitum, kept at an ambient temperature of (23±1)°C, and a relative humidity of 60%±5%, and were pre-raised for 1 week. Rats were randomly divided into 3 groups, 8 in each group, which were blank group, milk powder base group and anti-hypertensive milk powder group, and normal saline (10mL / kgm b ) and 0.35g / kg m b ...

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Abstract

The invention discloses an ACE inhibitory peptide and a preparation method thereof, and provides a casein hydrolysate which has an anti-hypertension effect and is rich in the ACE inhibitory peptide, the ACE inhibitory peptide with an anti-hypertension effect, and a preparation method of the ACE inhibitory peptide. The casein hydrolysate rich in the ACE inhibitory peptide contains two peptide fragments with ACE inhibitory activities; and the amino acid sequences of the two peptide fragments with ACE inhibitory activities are respectively YS and YQKFPQYLQY. The amino acid sequence of the ACE inhibitory peptide is YS or YQKFPQYLQY. The ACE inhibitory peptide or the casein hydrolysate rich in the ACE inhibitory peptide is sufficiently mixed with milk powder matrixes to prepare anti-hypertension milk powder; or the ACE inhibitory peptide or the casein hydrolysate rich in the ACE inhibitory peptide is mixed with fresh milk to obtain a mixture, and lactic acid bacteria is inoculated in the mixture to obtain yoghourt with an anti-hypertension effect. The dairy product has a remarkable anti-hypertension effect, has no influence on the normal blood pressure, is safe to eat, and is suitable for being ate by the hypertension groups.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method of hydrolyzing bovine milk casein with pepsin and trypsin to prepare a peptide with ACE inhibitory activity, and adding it to the milk powder matrix to produce a peptide with antihypertensive effect milk powder; or added to sterilized fresh milk, yogurt with antihypertensive effect obtained through lactic acid fermentation. Background technique [0002] With the development of the world and the progress of society, people's living standards continue to improve; people's lifestyles and dietary structures have undergone tremendous changes, and modern civilization diseases have also followed. [0003] Diseases of modern civilization mainly include obesity, diabetes, high blood pressure, and depression. Among these common diseases, high blood pressure is one of the chronic diseases that seriously endanger human health, and it is also a common disease worldwide. Controlling ris...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K5/00C07K7/06C12P21/06A23C9/152A23C9/13
Inventor 胡志和杨铭王佳佳薛璐吴光磊赵勇
Owner TIANJIN UNIV OF COMMERCE
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