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Walnut protein isolate, preparation method thereof, phosphorylated walnut protein isolate, preparation method of phosphorylated walnut protein isolate, and food

A protein separation and phosphorylation technology, applied in the food field, can solve the problems of high technical and equipment requirements and high cost, and achieve the effects of simplifying the process, avoiding loss and damage, and reducing costs

Active Publication Date: 2020-11-27
XIHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the reverse micellar method is currently relatively expensive and requires high technology and equipment

Method used

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  • Walnut protein isolate, preparation method thereof, phosphorylated walnut protein isolate, preparation method of phosphorylated walnut protein isolate, and food
  • Walnut protein isolate, preparation method thereof, phosphorylated walnut protein isolate, preparation method of phosphorylated walnut protein isolate, and food
  • Walnut protein isolate, preparation method thereof, phosphorylated walnut protein isolate, preparation method of phosphorylated walnut protein isolate, and food

Examples

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preparation example Construction

[0078] The present application proposes a preparation method of walnut protein isolate, which may include the following steps: leaching defatted walnuts with a salt solution.

[0079] Wherein, the protein content of the defatted walnut can be 50-60wt%.

[0080] In an optional embodiment, before leaching, the defatted walnuts can also be pulverized to obtain defatted walnut powder, so as to improve the leaching effect. Further, before soaking, the pulverized defatted walnuts can also be sieved, such as through a 60-100 mesh sieve.

[0081] In an optional embodiment, during leaching, the amount of salt solution and defatted walnuts can be 10-20mL: 1g, such as 10mL: 1g, 12mL: 1g, 15mL: 1g, 18mL: 1g or 20mL: 1g, etc., also Other solid-liquid ratios within the range of 10-20mL: 1g can be used.

[0082] In alternative embodiments, the saline solution may be an aqueous or buffered saline solution.

[0083] For reference, when the salt solution is an aqueous salt solution, the conc...

Embodiment 1

[0122] The method for preparing walnut protein isolate powder by leaching with different salt concentrations comprises the following steps:

[0123] After the walnuts are shelled, the walnut kernels are peeled and washed.

[0124] The walnut oil is extracted by cold pressing method or supercritical extraction technology, and the walnut pulp after deoiling is further extracted by n-hexane or petroleum ether and then dried to obtain defatted walnuts.

[0125] The defatted walnuts are pulverized with a pulverizer and passed through a 100-mesh sieve to obtain defatted walnut powder. Mix defatted walnut powder with NaCl concentration of 0.1, 0.3, 0.5, 0.75, 1, 1.5, 2, 2.5, 3, 3.5 and 4mol / L aqueous solution or pure water according to the mass-volume ratio of material to liquid in the ratio of 1g:20mL Uniformly, placed at 25 ° C for 1 hour shaking extraction.

[0126] Solid-liquid separation: Centrifuge the extract at 10,000 g for 30 minutes at room temperature to remove insoluble...

Embodiment 2

[0132] The method for preparing walnut protein isolate powder by extracting different types of salts comprises the following steps:

[0133] After the walnuts are shelled, the walnut kernels are peeled and washed.

[0134] Walnut oil is extracted by cold pressing or supercritical extraction technology, and the deoiled walnut meal is further extracted with n-hexane or petroleum ether to degrease and dry to obtain defatted walnuts.

[0135] The defatted walnuts are pulverized by a pulverizer and passed through a 100-mesh sieve to obtain defatted walnut powder. Mix defatted walnut powder with 1mol / L NaCl, KCl, MgCl 2 , CaCl 2 , NaAc, NaHCO 3 、Na 2 CO 3 、Na 2 SO 4 and K 2 HPO 4 The aqueous solution was mixed evenly at a ratio of 1g:20mL according to the mass-to-volume ratio of the material to liquid, and placed at 25°C for oscillating extraction for 1 hour.

[0136] Solid-liquid separation: Centrifuge the extract at 10,000 g for 30 minutes at room temperature to remove i...

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Abstract

The invention discloses a walnut protein isolate, a preparation method thereof, a phosphorylated walnut protein isolate, a preparation method of the phosphorylated walnut protein isolate, and a food,and belongs to the technical field of foods. The preparation method of the walnut protein isolate comprises the step of extracting degreased walnuts with a salt solution. The method has the advantagesof low cost, simple process and mild conditions, is beneficial to maintaining the natural structure of the protein, prevents the influence of acid-base and other extreme conditions on the protein conformation, and avoids the loss and damage of the protein. The obtained walnut protein isolate is high in protein content. The phosphorylated walnut protein isolate is prepared by carrying out phosphorylation treatment on the walnut protein isolate, or directly carrying out simultaneous extraction and phosphorylation treatment on degreased walnuts by using a phosphorylated reagent. The obtained phosphorylated walnut protein isolate has relatively high solubility and emulsifying activity. The food, prepared from the raw materials of the walnut protein isolate or the phosphorylated walnut proteinisolate, has a certain antioxidant or antihypertensive effect.

Description

technical field [0001] The invention relates to the technical field of food, in particular to a walnut protein isolate and a preparation method thereof, a phosphorylated walnut protein isolate and a preparation method thereof, and a food. Background technique [0002] Walnut is an important economic nut crop, and China is the largest producer of walnut in the world. Fresh walnuts contain 15-20% protein, among which the proportion of eight essential amino acids required by the human body is reasonable, and it is an important source of plant protein. [0003] Although walnut production ranks first in the world, the deep processing of walnut products in China is relatively lacking, and walnut oil is mainly produced by pressing. Depending on the way walnut oil is extracted, the protein content in the remaining walnut meal is as high as 50-60%. Walnut meal as a high-protein nutrient substance can often only be used as a raw material for livestock feed. At the same time, commer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/14C07K1/107C07K1/34C07K1/36A23L33/185
CPCC07K1/145C07K1/1077C07K1/34C07K1/16A23L33/185A23V2002/00A23V2200/30A23V2200/326A23V2250/548
Inventor 周拯严春俊
Owner XIHUA UNIV
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