Detection method for CVA10 in environmental water body

A detection method and virus technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, fluorescence/phosphorescence, etc., can solve the problem that the cell culture method is difficult to distinguish and identify, the CVA10 cannot be accurately qualitatively or quantitatively detected, and the factors affecting the detection are complex. And other issues

Inactive Publication Date: 2013-09-18
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because there are a variety of pathogenic microorganisms in the environmental water, especially other enteroviruses, these microorganisms interfere with the detection of CVA10 by cell culture, and it is difficult to distinguish and identify them by cell culture; PCR method is widely used in the detection of CVA10, but now Some PCR detection tech

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Detection method for CVA10 in environmental water body
  • Detection method for CVA10 in environmental water body
  • Detection method for CVA10 in environmental water body

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0075] The equipment and reagents used in this embodiment are as follows:

[0076] (1) High-speed refrigerated centrifuge with a maximum speed of 14000r / min;

[0077] (2) 4℃, -80℃ refrigerator

[0078] (3) Centrifuge tubes, 50mL, 1.5mL, 0.5mL, 0.2mL;

[0079] (4) Trace ultraviolet spectrophotometer;

[0080] (5) Constant temperature metal heater

[0081] (6) Bacterial shaker, bacterial incubator

[0082] (7) Real-time fluorescent quantitative PCR instrument;

[0083] (8) Gradient qualitative PCR instrument;

[0084] (9) Electrophoresis apparatus;

[0085] (10) Gel Imager

[0086] (11) pMD19-T vector;

[0087] (12) Competent Escherichia coli DH5α:

[0088] (13) Hydrochloric acid, NaCl solution, MgCl 2 solution, etc.;

[0089] (14) Polyethylene glycol (PEG-6000) with a molecular weight of 6000, glucose, etc.;

[0090] (15) 5mol / L NaCl solution;

[0091] (16) IPTG / X-gal / Amp plate;

[0092] (17) LB liquid medium;

[0093] (20) Total RNA extraction kit;

[0094] (18...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a detection method for CVA10 in an environmental water body, wherein a semi-nested RT-PCR (Reverse Transcription-Polymerase Chain Reaction) and real-time fluorescence quantification RT-PCR reaction system is established to detect CVA10 virus in the environmental water body by using a recombinant plasmid as a reference product, and the recombinant plasmid is prepared by using a primer and a probe aiming at CVA10 virus designed according to a genetic conservative fragment of enterovirus belonging to a VP1 serotype area. The method has good specificity and high sensitivity and precision, and can qualitatively and quantitatively detect CVA10 virus in the environmental water body accurately.

Description

technical field [0001] The invention belongs to the technical field of detection of environmental water quality, and in particular relates to a detection method of CVA10 virus in environmental water. technical background [0002] Coxsackievirus A10 (CVA10) is one of the main pathogens causing hand, foot and mouth disease, belonging to the Enterovirus genus. CVA10 is a water-borne virus that can be released into environmental waters from the secretions of CVA10-infected patients and can survive in environmental waters for a long time. The water body polluted by it becomes an important pollution source of CVA10, which can cause the outbreak of hand, foot and mouth disease. [0003] Existing methods for detecting CVA10 mainly include cell culture method and polymerase chain reaction (Polymerase Chain Reaction, PCR). Because there are a variety of pathogenic microorganisms in the environmental water, especially other enteroviruses, these microorganisms interfere with the detec...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/70C12Q1/68G01N21/64
Inventor 周进宏王晓昌张崇淼船水尚行佐野大辅
Owner XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap