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Cultivation method of Cordyceps militaris (pupa)

A cultivation method and technology of Cordyceps silkworm, applied in the field of cultivation of Cordyceps silkworm, can solve the problems of inappropriate cultivation conditions, cocooning and pupation, low sloppy production rate, etc., and achieve improved product added value, uniform size and shape, high sloppy production rate Effect

Inactive Publication Date: 2015-05-13
HESHAN ZHONGCHUN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been a lot of researches on the breeding methods of Cordyceps militaris, but these researches still have the following deficiencies: (1) Low infection rate of inoculation: Since silkworms have strong anti-infection ability to Cordyceps militaris, if the inoculation method is improper, it is easy to As a result, the parasites are not infected, and subsequent cultivation cannot be carried out; (2) Low ossification rate: during the cultivation process, cocooning and pupation occur, resulting in waste of resources; (3) Low yield rate: due to inappropriate cultivation conditions, (4) The appearance of silkworm caterpillar fungus is not beautiful: due to the lack of standardized treatment in the cultivation process, the individual size of the product is different, and the growth position of the fruiting body is different. The shape is too different from the natural Cordyceps, which affects the high-end packaging of Cordyceps, which is not conducive to increasing the added value of the product

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Selection of live silkworms

[0023] Select silkworms of 3 to 5 years old, the bigger the better.

[0024] (2) Preparation of Cordyceps militaris spore suspension

[0025] The Cordyceps militaris strain covered with mycelium was eluted with sterile water to prepare a spore suspension with a concentration of 200 mg / mL, which was stored at low temperature for future use.

[0026] (3) Low temperature anesthesia before inoculation

[0027] The live silkworms were placed in a constant temperature incubator and placed at 4-6°C for 1 day without feeding, which made the silkworms enter a semi-dormant state due to low temperature and starvation, and their life activities were reduced.

[0028] (4) Vaccination

[0029] Use a sterilized syringe to extract the spore suspension, carefully pierce it with the head of the silkworm, and slowly inject about 2 mL. And spray a small amount of spore suspension on the silkworm body surface.

[0030] The experimental results showed t...

Embodiment 2

[0036] (1) Selection of live silkworms

[0037] Select silkworms of 3 to 5 years old, the bigger the better.

[0038] (2) Preparation of Cordyceps militaris spore suspension

[0039] The Cordyceps militaris strain covered with mycelium was eluted with sterile water to prepare a spore suspension with a concentration of 300 mg / mL, which was stored at low temperature for future use.

[0040] (3) Low temperature anesthesia before inoculation

[0041] The live silkworms were placed in a constant temperature incubator and placed at 4-6°C for 2 days without feeding during the period, so that the silkworms entered a semi-dormant state due to low temperature and starvation, and their life activities were reduced.

[0042] (4) Vaccination

[0043] Use a sterilized syringe to extract the spore suspension, carefully pierce it with the head of the silkworm, and slowly inject about 3 mL. And spray a small amount of spore suspension on the silkworm body surface.

[0044] The experimenta...

Embodiment 3

[0050] (1) Selection of live silkworms

[0051] Select silkworms of 3 to 5 years old, the bigger the better.

[0052] (2) Preparation of Cordyceps militaris spore suspension

[0053] The Cordyceps militaris strain covered with mycelium was eluted with sterile water to prepare a spore suspension with a concentration of 400 mg / mL, which was stored at low temperature for future use.

[0054] (3) Low temperature anesthesia before inoculation

[0055] The live silkworms were placed in a constant temperature incubator and placed at 4-6°C for 3 days without feeding during the period, which caused the silkworms to enter a semi-dormant state due to low temperature and starvation, and their life activities were reduced.

[0056] (4) Vaccination

[0057] Use a sterilized syringe to extract the spore suspension, carefully pierce it with the head of the silkworm, and slowly inject about 4 mL. And spray a small amount of spore suspension on the silkworm body surface.

[0058] The exper...

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PUM

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Abstract

The invention discloses a cultivation method of Cordyceps militaris (pupa). The method includes the steps of 1, subjecting properly-aged live silkworms to crymo-anesthesia; 2, pouring pore suspension of Cordyceps militaris into the heads of the silkworms; 3, cultivating the treated and survived silkworms at 4-6 DEG C under the humidity of 65-75% until death and stiffing; 4, embedding the dead, stiff silkworms in sandy soil to cultivate for 6-8 days; 5, cultivating at 17-19 DEG C under the humidity higher than 75%, decreasing the humidity to 60-70% when obvious mycelia grow from the heads and primordium of fruiting bodies differentiates, continuing to cultivate until the length of the fruiting bodies on the heads of the silkworms grow to more than 2cm long, and taking out Cordyceps militaris (pupa) before drying. The method has the advantages that inoculation infection rate, stiffing rate and yield are high; nutrients of the obtained product are approximate to those of natural Cordyceps; the shape of the obtained product is approximate to that of wild Cordyceps; the fruiting bodies are full and golden yellow with uniform size, so that standard packaging is facilitated and added value of products is higher.

Description

technical field [0001] The invention belongs to the technical field of cordyceps cultivation, and in particular relates to a cultivation method of silkworm caterpillar fungus. Background technique [0002] Cordyceps militaris, also known as Cordyceps militaris or Cordyceps militaris, is an edible and medicinal fungus. Studies have shown that Cordyceps militaris is the same or similar to Cordyceps militaris in terms of nutritional value, active ingredients and health care efficacy, but it does not require a special habitat and specific host like Cordyceps militaris, so the development and application of Cordyceps militaris has a huge market. At present, the research and development of Cordyceps militaris mainly includes three aspects: fermented mycelium, rice fruiting body and silkworm fungus. The fermented mycelium and rice fruiting bodies have no sclerotium because they only have sporozoites, which affects the health care efficacy and commodity value to a certain extent. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G1/04
Inventor 项坤
Owner HESHAN ZHONGCHUN BIOTECH
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