Cultivation method of Cordyceps militaris (pupa)
A cultivation method and technology of Cordyceps silkworm, applied in the field of cultivation of Cordyceps silkworm, can solve the problems of inappropriate cultivation conditions, cocooning and pupation, low sloppy production rate, etc., and achieve improved product added value, uniform size and shape, high sloppy production rate Effect
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Embodiment 1
[0022] (1) Selection of live silkworms
[0023] Select silkworms of 3 to 5 years old, the bigger the better.
[0024] (2) Preparation of Cordyceps militaris spore suspension
[0025] The Cordyceps militaris strain covered with mycelium was eluted with sterile water to prepare a spore suspension with a concentration of 200 mg / mL, which was stored at low temperature for future use.
[0026] (3) Low temperature anesthesia before inoculation
[0027] The live silkworms were placed in a constant temperature incubator and placed at 4-6°C for 1 day without feeding, which made the silkworms enter a semi-dormant state due to low temperature and starvation, and their life activities were reduced.
[0028] (4) Vaccination
[0029] Use a sterilized syringe to extract the spore suspension, carefully pierce it with the head of the silkworm, and slowly inject about 2 mL. And spray a small amount of spore suspension on the silkworm body surface.
[0030] The experimental results showed t...
Embodiment 2
[0036] (1) Selection of live silkworms
[0037] Select silkworms of 3 to 5 years old, the bigger the better.
[0038] (2) Preparation of Cordyceps militaris spore suspension
[0039] The Cordyceps militaris strain covered with mycelium was eluted with sterile water to prepare a spore suspension with a concentration of 300 mg / mL, which was stored at low temperature for future use.
[0040] (3) Low temperature anesthesia before inoculation
[0041] The live silkworms were placed in a constant temperature incubator and placed at 4-6°C for 2 days without feeding during the period, so that the silkworms entered a semi-dormant state due to low temperature and starvation, and their life activities were reduced.
[0042] (4) Vaccination
[0043] Use a sterilized syringe to extract the spore suspension, carefully pierce it with the head of the silkworm, and slowly inject about 3 mL. And spray a small amount of spore suspension on the silkworm body surface.
[0044] The experimenta...
Embodiment 3
[0050] (1) Selection of live silkworms
[0051] Select silkworms of 3 to 5 years old, the bigger the better.
[0052] (2) Preparation of Cordyceps militaris spore suspension
[0053] The Cordyceps militaris strain covered with mycelium was eluted with sterile water to prepare a spore suspension with a concentration of 400 mg / mL, which was stored at low temperature for future use.
[0054] (3) Low temperature anesthesia before inoculation
[0055] The live silkworms were placed in a constant temperature incubator and placed at 4-6°C for 3 days without feeding during the period, which caused the silkworms to enter a semi-dormant state due to low temperature and starvation, and their life activities were reduced.
[0056] (4) Vaccination
[0057] Use a sterilized syringe to extract the spore suspension, carefully pierce it with the head of the silkworm, and slowly inject about 4 mL. And spray a small amount of spore suspension on the silkworm body surface.
[0058] The exper...
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