A method and reagent device for measuring anti-RA33 antibody IgG

Abstract

The present invention provides a method for determining anti-RA33 antibody by ELISA based on a special device. The device comprises a substrate, wherein 8 wells are formed on the substrate, and one end of the substrate is provided with a handle and the handle is adhered with a bar-shaped code with the information of detecting reagent. By using the method and device of present invention, detection reagents can be placed in a single analysis device, thus the operation is simple and it is not easy to go wrong so that the correctness of the detection result is ensured.

Description

technical field [0001] The application of the present invention relates to a method and a reagent device for measuring anti-RA33 antibody IgG, which belong to the technical field of biological detection.  Background technique [0002] Rheumatoid arthritis (RA) is the most common systemic autoimmune disease, with chronic, symmetrical, joint-erosive synovitis as the main manifestation, most of the disease is progressive, if not treated in time, about one-third One of the patients will be disabled. Therefore, early diagnosis and early treatment are the key to the treatment of rheumatoid arthritis. The diagnostic criteria revised by the American College of Rheumatology (ACR) in 1987 are based on clinical manifestations, and rheumatoid factor (RF) is the only diagnostic criterion, and its specificity is poor. Therefore, rheumatologists are committed to finding biological markers for the early diagnosis of RA, and have achieved certain results.  [0003] The RA33 antibody w...

AI Technical Summary

Problems solved by technology

[0008] (1) Only qualitative and semi-quantitative analysis can be carried out, and the specific amount of the substance under inspection cannot be obtained;

[0009] (2) The operation steps are cumbersome, the test takes a long time, and it is difficult to carry out this test at the grassroots level;

[0010] (3) The sensitivity and specificity of detection need to be improved;

[0011] (4) At the same time, due to the complex and time-consuming process of extracting antigens, the requirements for equipment are high, and the antigens are difficult to store for a long time, which is not conducive to clinical promotion and application

[0015] (2) There can be as many as 11 kinds of reagents used for quantitative determination, and each detection reagent must be contained in a reagent bottle, and each time a reagent is used, the suction nozzle needs to be replaced to fill the microwell plate respectively. In the well, not only are there many types of reagent bottles, but also the operation of adding reagents is extremely cumbersome. If you do not use a fully automatic enzyme immunoassay analyzer, all operations must be done manually, and the price of a fully automatic enzyme immunoassay analyzer is very expensive. , a large investment;

[0016] (3) There is no barcode of reagent information for each test or each test. The production batch number and expiration date information of the test reagent can only be known or known by checking the label on the outer packaging box of the test kit, and the known information is not available during the test process. Controlled, with great discretion;

[0017] (4) The detection reagents are open during the detection process, which may easily cause cross-contamination between various reagents and affect the detection results;

[0018] (5) When the automatic enzyme immunoassay analyzer is not used for detection, the detection process is manual operation, the addition of reagents or samples is not very precise, the operation process is extremely cumbersome and complicated, and operation errors are prone to occur. poor precision;

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