Specific primer used for analysis of diversity of pathogenic fungi causing root rot of soybean

A technology for diversity analysis and soybean root rot, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of difficult detection and prevention of pathogenic fungi of soybean root rot, and achieve intuitive detection results. Diversity Analysis for Accurate Effects

Active Publication Date: 2013-11-27
黑龙江禾家福农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no specific primers for the diversity analysis of pathogenic fungi of soybean ro

Method used

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  • Specific primer used for analysis of diversity of pathogenic fungi causing root rot of soybean
  • Specific primer used for analysis of diversity of pathogenic fungi causing root rot of soybean
  • Specific primer used for analysis of diversity of pathogenic fungi causing root rot of soybean

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Experimental program
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Effect test

specific Embodiment approach 1

[0013] Embodiment 1: The nucleotide sequence of the specific primer used in the diversity analysis of soybean root rot pathogenic fungi in this embodiment is 5'-CGTTGGGTTATTTCCCCGGC-3'.

[0014] The specific primer of this embodiment is named as F.rr.

[0015] The nucleotide sequence of the unmodified base obtained from the 359bp to 377bp of S. cerevisiae18s rRNA is 5'-CGTTGGGTTATTTCCCCGTT-3'; it was named YUAN1.

specific Embodiment approach 2

[0016] Embodiment 2: In this embodiment, the specific primer F.rr and the reference primer YUAN1 of Embodiment 1 are used as reverse primers, and NS1 (5′-GTAGTCATATGCTTGTCTC-3′) in the universal primer pair is used as the forward primer respectively. Use paired primers to test.

[0017] Verification test: Fusarium oxysporum (F.Oxysporum), Fusarium solani (F.Solani), Fusarium graminearum (F.Graminearum), Fusarium oats (F.Avenaceum), Fusarium seminaked F.Semiectum, F.verticillioides, F.solani, F.coeruleum, F.equiseti ), Phytophthora spp. and Rhizoctonia solani (Phytophthora spp.) and Rhizoctonia solani (Rhizoctonia solani) 11 kinds of soybean root rot pathogenic fungi DNA samples and the mixed samples of the above 11 soybean root rot pathogenic fungal DNA samples were used as templates for verification experiments.

[0018] Interference test: Alternaria is a common disease fungus in Northeast China. Although it is not the pathogenic fungus of soybean root rot, it is very close ...

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Abstract

The invention discloses a specific primer used for analysis of diversity of pathogenic fungi causing root rot of soybean, which relates to a specific primer used for analysis of diversity of pathogenic fungi. The nucleotide sequence of the specific primer is 5'-CGTTGGGTTATTCCCCGGC-3'. The specific primer provided by the invention has the advantages of accurate analysis of diversity, visual, rapid and reliable detection results, etc. and can be applied in the field of microbiological detection.

Description

technical field [0001] The invention relates to a specific primer for analyzing the diversity of pathogenic fungi. Background technique [0002] Soybean root rot is a worldwide disease with wide distribution, serious damage and difficult control. It mainly infects the soybean cortex in the early stage, and eventually damages the lateral roots, seriously affecting the crop's absorption of water and nutrients. The composition of soybean root rot pathogens is complex. In Northeast my country, Fusarium Oxysporum, Fusarium Solani, Fusarium Graminearum and Fusarium Avenaceum are the main pathogens. and Fusarium Semiectum. Although molecular marker technology can be used to identify each soybean root rot pathogen separately, soybean soil often contains a variety of root rot pathogens. If control is to be carried out, diversity analysis must be carried out first, so as to know the soybean soil. Contains several different pathogenic fungi of soybean root rot, which are the pathogen...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11C12R1/77C12R1/645
Inventor 蔡柏岩韩洁接伟光
Owner 黑龙江禾家福农业科技有限公司
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