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Application of cyclocarya paliurus extract in preparation of medicament for preventing and treating leukemia

A technology for extract and leukemia, which is applied in the application field of the polysaccharide extract of pennywort in the preparation of drugs for preventing and treating leukemia, and achieves the effects of improving utilization rate and preventing and treating leukemia.

Active Publication Date: 2014-01-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Human chronic myelogenous leukemia cell K562 is often used in the study of lymphocyte killing activity, and the effect of Cyclocarya paliurus on lymphocyte killing of human leukemia cells has not yet been reported in literature and patents

Method used

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  • Application of cyclocarya paliurus extract in preparation of medicament for preventing and treating leukemia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The preparation of embodiment 1 Cyclocarya paliurus extract

[0027] (1) Extraction: Fresh Cyclocarya paliurus fallen leaves, sorted to remove impurities, cleaned with tap water, dried in a constant temperature oven at 70°C until the moisture content is below 13%, crushed through a 100-mesh sieve; added 10 times the quality of distilled water, extracted 3 times at 100°C for 1 hour each time, filtered, combined the filtrates, and concentrated with a rotary evaporator at a temperature of 45°C to a concentration of Cyclocarya paliurus containing 2 grams of raw dry material per ml. Extract I was obtained after freeze-drying;

[0028] (2) Degreasing and alcohol precipitation: the above-mentioned Cyclocarya paliurus extract Ⅰ, soaked in 10 times the amount of ethanol and refluxed for 3 times, filtered, evaporated the organic solvent, dissolved in an appropriate amount of water, added ethanol until the ethanol content was 80%, and placed in an ice bath overnight , filtered th...

Embodiment 2

[0032] The preparation of embodiment 2 Cyclocarya paliurus polysaccharide extract

[0033] (1) Extraction: fresh old leaves of Cyclocarya paliurus, sorted to remove impurities, cleaned with tap water, dried in a constant temperature oven at 70°C until the moisture content is less than 9%, crushed through a 100-mesh sieve; 10 times the mass of distilled water, extracted twice at 100°C for 1 hour each time, filtered, added 10 times the mass of water to the filter residue, extracted twice at 60°C, combined the filtrates, and concentrated to the equivalent per ml with a rotary evaporator at 45°C The extract Ⅰ was obtained after freeze-drying the concentrated solution of Cyclocarya paliurus containing 2 grams of raw material;

[0034] (2) Degreasing and alcohol precipitation: the above-mentioned Cyclocarya paliurus extract I was soaked and refluxed with 10 times the amount of 80% ethanol for 3 times, filtered, evaporated the organic solvent, dissolved in an appropriate amount of wate...

Embodiment 3

[0038] The preparation of embodiment 3 Cyclocarya paliurus polysaccharide extract

[0039] (1) Extraction: Take 2 kg of tender leaves of Cyclocarya paliurus after drying, crushing and sieving, add 8 times the quality of distilled water, pressurize at 120°C to extract twice, 20 minutes each time, filter, add 8 times the quality of water to the filter residue, 60 Extract twice at °C, combine the filtrates, concentrate to 1000 ml with a rotary evaporator at a temperature of 50 °C, that is, each ml is equivalent to the concentrated solution of Cyclocarya paliurus containing 2 g of raw dry material, and freeze-dry to obtain extract I;

[0040] (2) Degreasing and alcohol precipitation: the above-mentioned Cyclocarya paliurus extract I was soaked with 10 times the amount of 80% ethanol and refluxed for 6 hours, filtered, evaporated the organic solvent, dissolved in an appropriate amount of water, added ethanol until the ethanol content was 80%, and placed in an ice bath Stand for 24 ...

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Abstract

The invention discloses the application of cyclocarya paliurus extract in the preparation of a medicament for preventing and treating leukemia. High-content cyclocarya paliurus extract is obtained by performing the purification treatment combined of water extraction, ethanol extraction, membrane dialysis, and gel chromatographic column on cyclocarya paliurus leaves. The cyclocarya paliurus extract contains 90 to 98 percent of polysaccharide; killing activity detection experiments show that the cyclocarya paliurus extract can be used for improving the activity of the splenocytes for killing human leukemia cells K652 in vitro; the killing multiples can reach 2.47 to the maximum. After granules, tablets, buccal tablets, capsules, pills, powder injection or oral liquid prepared by adding certain additives into the cyclocarya paliurus extract disclosed by the invention are orally taken, the effects of preventing and treating the leukemia can be achieved.

Description

technical field [0001] The invention relates to the technical field of application of traditional Chinese medicines, in particular to the application of a polysaccharide extract of Cyclocarya paliurus in the preparation of medicines for preventing and treating leukemia. Background technique [0002] Cyclocarya paliurus (Bata1.) Iljinskaja (CPs), also known as green money plum, mountain willow, money tree, sweet tea tree, mountain tree, a string of money, etc., is a unique single-species plant of the genus Cyclocarya in the family Juglandaceae. Tall broad-leaved trees, unique to my country, belong to three types of endangered tree species, which are used for timber, greening, health care and medicine. Because the shape of the tree is like a willow, the fruit is round like a copper coin, and the color is green and drooping, so it is called "Qingqian Liu". [0003] Cyclocarya paliurus is produced in mountainous areas with an altitude of 420-2500m from the south of the Yangtze R...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/52A61K31/715A61P35/02
Inventor 陈云龙徐程
Owner ZHEJIANG UNIV
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