High dissolved oxygen bioreactor for high-density culture of genetically engineered bacteria and culture control method

A high-density culture and genetically engineered bacteria technology, applied in the field of high dissolved oxygen bioreactors, can solve the problems of unfavorable shear-sensitive microbial growth, unfavorable microbial growth, weak macro-mixing strength, etc. Good liquid dispersion performance and the effect of overcoming the lack of dissolved oxygen

CN103525689AInactive Publication Date: 2014-01-22NANJING UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2014-01-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a high dissolved oxygen bioreactor for the high-density culture of genetically engineered bacteria and a culture control method. The reactor comprises a tank body, wherein the top of the tank body is provided with a stirring driving motor, and an output shaft of the stirring driving motor is used for driving a stirring paddle and is connected with the stirring paddle; the bottom of the tank body is provided with a rotor driving motor, an output shaft of the rotor driving motor is used for driving a gas-liquid dispersion rotor and is connected with the gas-liquid dispersion rotor, the gas-liquid dispersion rotor is provided with an inner ventilation cavity which is provided with an air inlet at the top and an air outlet at the lower part, and the air inlet is connected with an under-pressure air source through a pipeline; the inner side at the bottom of the tank body is also fixedly connected with a gas-liquid dispersion stator provided with a diversion trench; the gas-liquid dispersion stator is located at the circumference of the gas-liquid dispersion rotor and is coaxial with the gas-liquid dispersion rotor to form a rotating pair. The method comprises a gas-liquid dispersion rotor ventilation control process, a gas-liquid dispersion rotor rotating speed control process and a stirring paddle stirring rotating speed control process. The high dissolved oxygen bioreactor is high in dissolved oxygen level and suitable for the high-density culture of the genetically engineered bacteria.
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Description

technical field

[0001] The invention relates to a high-dissolved oxygen bioreactor for high-density cultivation of genetically engineered bacteria and a cultivation control method using the reactor, belonging to the technical field of biological fermentation engineering. Background technique

[0002] With the development of genetic engineering technology, more and more biological products can be produced through genetic engineering process. Among them, Escherichia coli and Pichia pastoris have been widely used in the construction of genetically engineered bacteria to obtain exogenous gene products that are useful to people.

[0003] The use of genetically engineered bacteria for fermentation aims to obtain large quantities of high-quality exogenous gene products, and at the same time reduce the contamination of the host cell's own components as much as possible, so its fermentation process is different from that of traditional microorganisms.

[0004] The traditional batch ...

Examples

Embodiment

[0054] Such as Figure 1 to Figure 6 As shown, the high-dissolved oxygen bioreactor for the high-density cultivation of genetically engineered bacteria of the present embodiment comprises a tank body with feeding holes 4 in the upper part and a discharge port 15 in the lower part. The output shaft of the drive motor 6 is connected to the stirring paddle extending into the tank; the rotor drive motor 16 is arranged outside the bottom of the tank, and the output shaft of the rotor drive motor 16 is connected to the gas-liquid dispersion rotor 18 inside the tank to achieve gas-liquid dispersion. The rotor 18 has a ventilated inner chamber with an air inlet on the top and an air outlet on the bottom, and the air inlet is connected to a pressurized air source through a pipeline; The diversion groove of the water inlet and the water outlet is established; the gas-liquid dispersion stator 19 is located in the circumferential direction of the gas-liquid dispersion rotor 18, and the ga...

Embodiment example 1

[0096] The size of the reactor is: the volume of the tank is 5m 3 , the height-to-diameter ratio of the tank is 2.2:1, the diameter of the gas-liquid dispersion rotor is 215mm, the gas-liquid dispersion stator is designed with 16 diversion grooves, the outer diameter is 405mm, and the width of the four baffles is 140mm. There are two layers of stirring paddles with a diameter of 500 mm.

[0097] In this implementation case, under the condition of 2.8 tons of liquid, the critical air flow rate of the gas-liquid dispersion rotor at a speed of 2100r / min, the critical speed of the gas-liquid dispersion rotor at this air flow rate, and the maximum stirring speed were measured.

[0098] The measured critical ventilation volume is 134.4m 3 / h, according to 85% of the critical ventilation rate is 114.2m 3 / h air intake, the measured critical speed of the gas-liquid dispersion rotor is 1950r / min. The speed of the gas-liquid dispersion rotor is set to 120% of the critical speed, that ...

Embodiment example 2

[0103] The size of the reactor is: the volume of the tank is 5m 3 , the height-to-diameter ratio of the tank is 2.2:1, the diameter of the gas-liquid dispersion rotor is 215mm, the gas-liquid dispersion stator is designed with 16 diversion grooves, the outer diameter is 405mm, and the width of the four baffles is 140mm. There are two layers of stirring paddles with a diameter of 500 mm.

[0104] In this implementation case, under the condition of 2.8 tons of liquid, the critical air flow rate of the gas-liquid dispersion rotor at a speed of 2100r / min, the critical speed of the gas-liquid dispersion rotor at this air flow rate, and the maximum stirring speed were measured.

[0105] The measured critical ventilation volume is 134.4m 3 / h, according to 70% of the critical ventilation rate is 94m 3 / h air intake, the measured critical speed of the gas-liquid dispersion rotor is 1780r / min. The speed of the gas-liquid dispersion rotor is set to 120% of the critical speed, that is...