A method for improving silkworm cocoon output stability
A stable and high-yield technology, which is applied in the direction of pharmaceutical formulations, organic active ingredients, and medical preparations containing active ingredients, etc., can solve problems such as silkworm cocoon yield failure, beauveria, and polyhedrosis, and achieve low cost, Simple to implement and guaranteed stability
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Embodiment 1
[0019] Embodiment one: reduce nuclear polyhedrosis virus infection
[0020] (1) Dissolve 10 g of ribavirin, terbinafine hydrochloride and ammonium chloride mixture in 200 ml of water, spray 2.5 kg of mulberry leaves, and spray mulberry leaves with drug-free water at the same time, and feed them to 5th instar silkworms after drying (variety: Jingsong ╳ Haoyue) for 8 hours, the former is the treatment group and the latter is the control group;
[0021] (2) Mulberry leaves were impregnated with 10 per ml 8 and 10 7 The silkworm karyopolyhedron suspension, after drying slightly, fed the treatment group and control group silkworms of step (1) respectively for 8 hours, and then reared with common mulberry leaves;
[0022] (3) On the 3rd day of the 5th instar, the silkworms in the treatment group of step (2) and the control group were fed with the drug-sprayed mulberry leaves and water-sprayed mulberry leaves in step (1) for 8 hours, and then fed with common mulberry leaves;
[00...
Embodiment 2
[0027] Embodiment two: reduce plasmotype polyhedrosis virus infection
[0028] (1) Dissolve 7.5 g of ribavirin, terbinafine hydrochloride and ammonium chloride mixture in 250 ml of water, spray 2 kg of mulberry leaves, and spray mulberry leaves with drug-free water at the same time, and feed them to 4th instar silkworms after drying (species: Jingsong ╳ Haoyue) for 8 hours, the former is the treatment group and the latter is the control group;
[0029] (2) Mulberry leaves were impregnated with 10 per ml 7 and 10 6 The silkworm cytoplasmic polyhedron suspension, after drying slightly, fed the treatment group and control group silkworms of step (1) respectively for 8 hours, and then reared with common mulberry leaves;
[0030] (3) On the 3rd day of the 4th instar, the silkworms in the treatment group of step (2) and the control group were fed with the drug-sprayed mulberry leaves of step (1) and the water-sprayed mulberry leaves for 8 hours, and then fed with common mulberry l...
Embodiment 3
[0035] Embodiment three: reduce Beauveria bassiana infection
[0036] (1) 5th instar silkworm (species: Jingsong ╳ Haoyue) body spray contains 10 per ml 10 Suspension of conidia of Beauveria bassiana;
[0037] (2) Divide the silkworms in step (1) into a treatment group and a control group;
[0038] (3) Treatment group silkworm: 10 g of ribavirin, terbinafine hydrochloride and ammonium chloride mixture was dissolved in 250 ml of water, sprayed with 2 kg of mulberry leaves, dried and fed for 12 hours;
[0039] Control group silkworm: Spray 2kg mulberry leaves with 250ml of clear water, dry and feed for 12 hours; then feed with common mulberry leaves;
[0040] (4) On the 3rd and 5th days of the 5th instar, the silkworms in the treatment group and the control group in step (3) were fed with medicine-sprayed mulberry leaves and water-sprayed mulberry leaves for 8 hours, and were fed with ordinary mulberry leaves until the clusters were raised at other times. The incidence of Bea...
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