Extraction method of land slug and anti-lung cancer application of land slug

An extraction method and slug technology are applied in the field of anti-lung cancer applications and slug extraction, can solve problems such as lack, and achieve the effects of good stability, high tumor inhibition rate and high purity

Active Publication Date: 2014-03-05
GUANGZHOU HANFANG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the lack of basic research on slug materials, the method of cutting molecular weight segments is used to separate and enrich the effective substances

Method used

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  • Extraction method of land slug and anti-lung cancer application of land slug
  • Extraction method of land slug and anti-lung cancer application of land slug
  • Extraction method of land slug and anti-lung cancer application of land slug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The slug was vacuum freeze-dried, added purified water and stirred in a water bath at 50°C for 4 hours, then added 85% ethanol, and stirred evenly until the ethanol concentration was 60-70%. After standing at 30°C for 24 hours, the solid was separated by centrifugation. The centrifugate was separated and retained by a 50nm inorganic ceramic membrane to obtain the total slug clear liquid (the separation frequency of the inorganic ceramic membrane system is 33.35Hz, the membrane inlet pressure is 1.5bar, and the membrane outlet pressure is 1bar). The total slug liquid is concentrated by reverse osmosis membrane (the frequency of reverse osmosis membrane filtration is 50Hz, the membrane inlet pressure is 28bar, and the membrane outlet pressure is 26bar). The slug powder mixture is calculated by fresh slug weight, and the yield is 0.3%.

[0028] Put the dried slug powder directly into the capsule shell in an amount of 0.3g to obtain the finished slug powder sample HKY.

Embodiment 2

[0030] The slug was vacuum freeze-dried, added purified water and stirred in a water bath at 50°C for 4 hours, then added 95% ethanol, and stirred evenly until the ethanol concentration was 60-70%. After standing at 30°C for 24 hours, the solid was separated by centrifugation. The centrifugate was separated and retained by a 500nm inorganic ceramic membrane to obtain the total slug supernatant (the separation frequency of the inorganic ceramic membrane system was 38.05Hz, the membrane inlet pressure was 1.9bar, and the membrane outlet pressure was 1.25bar). The total slug liquid is concentrated by reverse osmosis membrane (the frequency of reverse osmosis membrane filtration is 50Hz, the membrane inlet pressure is 22bar, and the membrane outlet pressure is 22bar). The slug powder mixture is calculated by fresh slug weight, and the yield is 0.1%.

[0031] Put the dried slug powder directly into the capsule shell in an amount of 0.3g to obtain the finished product.

Embodiment 3 Embodiment 1

[0032] The active experiment of embodiment 3 embodiment 1

[0033] The effect of the slug powder sample HKY (Example 1) in treating lung cancer will be described below through pharmacodynamic tests.

[0034] (1) Experimental materials and groups

[0035] Experimental animals: source, species, strain: SPF grade BALB / c nude mice.

[0036] Age in days: 28-35 days.

[0037] Gender: half male and half male.

[0038] Human cancer cell line: lung adenocarcinoma A549, provided by the Cell Bank of the Experimental Animal Center of Sun Yat-sen University.

[0039] Transplanted tumor: Human-derived lung adenocarcinoma A549 cell line was established by inoculating under the axillary skin. The amount of cell seeding is 6 tubes, and the number of each tube is about 1×10 7 . After inoculation to form xenograft tumors, it was used after 3 passages in nude mice.

[0040] Experimental drugs: HKY, intragastric administration once a day, provided by Guangzhou Baiyunshan Hanfang Modern Phar...

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Abstract

The invention discloses an extraction method of a land slug and an anti-lung cancer application of the land slug. The extraction method comprises the following steps: (1) carrying out vacuum freeze-drying on the land slug, adding purified water and then carrying out water bath agitation, adding a high-concentration ethanol solution, evenly agitating until the ethanol concentration is 60-70%, standing and then carrying out solid-liquid separation; (2) clarifying and intercepting the liquid obtained in the step (1) by adopting an inorganic ceramic membrane system, so as to obtain total clear liquid of the land slug; (3) concentrating the total clear liquid of the land slug obtained in the step (2) by a reverse osmosis membrane; and (4) concentrating and drying ultra-filtrate obtained in the step (3), so as to obtain water-soluble land slug powder. The molecular weight of the land slug powder obtained by the method is concentrated between 20,000 and 30,000, and has a significant antitumor effect; the tumor control rate is higher than that of cinobufagin and close to that of cyclophosphamide. The water-soluble land slug powder disclosed by the invention is used for treating a lung cancer, and is efficient and low-toxicity.

Description

technical field [0001] The technical field of slugs of the present invention, in particular, relates to a method for extracting slugs and its anti-lung cancer application. Background technique [0002] For nearly half a century, the morbidity and mortality of lung cancer have been increasing year by year. By the end of the 20th century, lung cancer had accounted for the first place in the death of malignant tumors. According to the data regularly released by the World Health Organization (WHO), the incidence and mortality of lung cancer are on the rise all over the world, especially in industrially developed countries. All kinds of pollution, such as vehicle exhaust and smoking are also the causes of lung cancer. Lung cancer is the most common malignant tumor in many developed countries. It ranks first among the common malignant tumors in men and ranks second in the common malignant tumors in women. According to survey statistics released by 28 developed countries, lung ca...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/56A61P35/00A61P11/00A61K35/618
Inventor 蒋兆健谢金魁刘洁瑜罗爱勤张聪琪李菁葛发欢
Owner GUANGZHOU HANFANG PHARMA
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