Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Epidermal cell enriching instrument and dynamic enriching method thereof

A technology of epidermal cells and enrichment instrument, applied in cell dissociation methods, epidermal cells/skin cells, animal cells, etc., can solve problems such as poor mixing, uneven digestion, long digestion time, etc., to achieve uniform digestion and promote Wound healing, fully rational use of the effect

Active Publication Date: 2014-03-05
ZHENDE MEDICAL CO LTD
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of static digestion are: poor mixing between digestive enzymes and substrate, long digestion time, and uneven digestion

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Epidermal cell enriching instrument and dynamic enriching method thereof
  • Epidermal cell enriching instrument and dynamic enriching method thereof
  • Epidermal cell enriching instrument and dynamic enriching method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Such as Figure 1-3 As shown, an epidermal cell enrichment instrument includes a base 1, and a control panel 2 arranged on the base 1, a digestion vessel 3, a filter vessel 4, a mixing vessel 5, a control unit 6, a heater 7, a vibrator 8, Sensor 9 and power supply 10 etc., wherein, control panel 2 comprises display screen 21 and button 22, in the present embodiment, above-mentioned display screen 21 adopts nixie tube display, is mainly used for displaying parameters such as temperature, vibration frequency, cycle time, and button 22 includes a power start button and a vibration start button. The vibrator 8 is installed at the bottom of the digestion vessel 3 to cause the digestion vessel 3 to vibrate at a certain frequency; in this embodiment, the vibrator 8 adopts a vibration motor, and the speed range of the vibration motor is 1100-2500rpm. The vibrator 8 can also be replaced by other similar parts, as long as the solution in the digestion vessel 3 can vibrate or sha...

Embodiment 2

[0054] The structure of an epidermal cell enrichment instrument is the same as in Example 1, the main difference is that the filter screen is 50 mesh.

[0055] A method for dynamically enriching epidermal cells, comprising pretreatment, digestion, filtration and liquid preparation, the specific steps are:

[0056] (1) Pretreatment: take the epidermis, and make several epidermal slices on the console 13 of the epidermal cell enrichment instrument; Medium-thick skin with a thickness of 0.5mm;

[0057] (2) Digestion: Start the power supply 10 of the epidermal cell enrichment instrument, take the epidermal sheet obtained in step (1) and place it in the digestion container 3, add digestive enzymes, control the vibrator 8 and the heater 7 through the control unit 6, and perform dynamic digestion. Obtain cell suspension; in this example, every 1.5 cm 2 Add 5ml of digestive enzymes to the sliced ​​skin, the digestive enzymes are formulated from trypsin and EDTA, wherein the mass co...

Embodiment 3

[0061] The structure of an epidermal cell enrichment instrument is the same as in Example 1, the main difference is that the filter screen is 20 mesh.

[0062] A method for dynamically enriching epidermal cells, including pretreatment, digestion, filtration and liquid preparation, the specific steps are:

[0063] (1) Pretreatment: take the epidermis, and make several epidermal slices on the console 13 of the epidermal cell enrichment instrument; the epidermis taken is a tomographic slice, and the particle size of each prepared epidermal slice is 5mm. A medium-thick skin with a thickness of 0.6mm;

[0064] (2) Digestion: Start the power supply of the epidermal cell enrichment instrument, take the epidermal sheet obtained in step (1) and place it in a digestion container, add digestive enzymes, control the vibrator 8 and heater 7 through the control unit 10, and perform dynamic digestion to obtain cells Suspension; in this example, every 2 cm 2 Add 6ml of digestive enzymes to...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Thicknessaaaaaaaaaa
Thicknessaaaaaaaaaa
Login to View More

Abstract

The invention discloses an epidermal cell enriching instrument and a dynamic enriching method thereof, belonging to the medical field. The epidermal cell enriching instrument comprises a base, and a control panel, a digestion container, a filter container, a mixing container, a control unit, a heater, vibrator, a sensor, a power supply, and the like which are arranged on the base. On one hand, a dynamic enriching digestion method can quickly disperse epidermis to obtain high-density epidermal cell suspension, on the other hand, the dynamic enriching digestion method can greatly lower using concentration and using amount of digestive enzyme; meanwhile, digestion time can be shortened, maintaining of cell activity is facilitated, cell loss is reduced; differentiated epidermal cells obtained by final liquor preparation are sprayed on a wound surface, epidermal stem cells are formed by the epidermal cells on the wound surface by virtue of retro-differentiation to promote the wound to heal, so that an epidermal source is sufficiently and reasonably utilized, and amplification multiple reaches 100-120 times.

Description

technical field [0001] The invention relates to a cell enrichment instrument and an enrichment method thereof, in particular to an epidermis cell enrichment instrument and a dynamic enrichment method thereof. Background technique [0002] In recent years, the incidence of burns in my country has been 2‰~10‰ of the total population. There are nearly ten million people with burns every year, and hundreds of thousands of people need to be hospitalized. After the burns are cured, a large number of scars are often left behind. Appearance, body shape, as well as functional and psychological aspects have a negative impact. [0003] At present, there are two main types of treatment for burn wounds with a large area using limited skin sources: [0004] 1. Particle skin technology: use physical methods to disperse limited autologous skin sources into as small particles as possible, and then replant them on the patient's wound surface. This technology can expand the skin graft by 10 to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12M1/38C12M1/36C12N5/07
CPCC12M47/04C12N5/0625C12N2509/00
Inventor 韩春茂胡信雷鲁建国韩阳
Owner ZHENDE MEDICAL CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com